{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T23:40:10Z","timestamp":1787787610511,"version":"build-2784847793"},"reference-count":47,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,8,1]],"date-time":"2009-08-01T00:00:00Z","timestamp":1249084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ministry of Education and Science of Spain","award":["SAF2006-14207-C02"],"award-info":[{"award-number":["SAF2006-14207-C02"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:p>\n                    Fifty\u2010three faecal samples from yellow\u2010legged gulls (\n                    <jats:italic>Larus cachinnans<\/jats:italic>\n                    ) at the Berlengas nature reserve in Portugal were cultured on Levine agar plates not supplemented with antimicrobial agents, and one Escherichia coli colony was isolated and identified from each sample. The percentages of resistant isolates for each of the drugs were ampicillin (43\u00b74 per cent), tetracycline (39\u00b76 per cent), nalidixic acid (34\u00b70 per cent), streptomycin (32\u00b71 per cent), trimethoprimsulfamethoxazole (SXT) (26\u00b74 per cent), ciprofloxacin (18\u00b79 per cent), chloramphenicol (18\u00b79 per cent), gentamicin (7\u00b75 per cent), tobramycin (7\u00b75 per cent) amikacin (5\u00b77 per cent) and amoxicillin\u2010clavulanic acid (1\u00b79 per cent). All the isolates were susceptible to cefoxitin, ceftazidime, cefotaxime, aztreonam and imipenem. The following resistance genes were detected:\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>TEM<\/jats:sub>\n                    (17 of 23\u00a0ampicillin\u2010resistant isolates),\n                    <jats:italic>tet<\/jats:italic>\n                    (A) and\/or\n                    <jats:italic>tet<\/jats:italic>\n                    (B) (18 of 21 tetracycline\u2010resistant isolates), aadA (12 of 17 streptomycin\u2010resistant isolates), cmlA (all chloramphenicol\u2010resistant isolates), aac(3)\u2010II with or without aac(3)\u2010IV (all four gentamicinresistant isolates), and\n                    <jats:italic>sul1<\/jats:italic>\n                    and\/or\n                    <jats:italic>sul2<\/jats:italic>\n                    and\/or\n                    <jats:italic>sul3<\/jats:italic>\n                    (all 14 SXT\u2010resistant isolates). The\n                    <jats:italic>intI1<\/jats:italic>\n                    gene was detected in 10 of 14 SXT\u2010resistant isolates, and three of them also contained class 2\u00a0integrons; four different gene cassette arrangements were identified among class 1\u00a0integrons (\n                    <jats:italic>aadA<\/jats:italic>\n                    ,\n                    <jats:italic>dfrA1<\/jats:italic>\n                    +\n                    <jats:italic>aadA1<\/jats:italic>\n                    ,\n                    <jats:italic>dfrA12<\/jats:italic>\n                    +\n                    <jats:italic>orfF<\/jats:italic>\n                    +\n                    <jats:italic>aadA2<\/jats:italic>\n                    and\n                    <jats:italic>sat<\/jats:italic>\n                    +\n                    <jats:italic>psp<\/jats:italic>\n                    +\n                    <jats:italic>aadA2<\/jats:italic>\n                    ) and one among the class 2\u00a0integrons (\n                    <jats:italic>dfrA1<\/jats:italic>\n                    +\n                    <jats:italic>sat<\/jats:italic>\n                    +\n                    <jats:italic>aadA1<\/jats:italic>\n                    ). Ninety per cent of the isolates were included in the A or B1\u00a0phylogenetic groups.\n                  <\/jats:p>","DOI":"10.1136\/vr.165.5.138","type":"journal-article","created":{"date-parts":[[2010,10,5]],"date-time":"2010-10-05T17:44:04Z","timestamp":1286300644000},"page":"138-142","source":"Crossref","is-referenced-by-count":44,"title":["Antimicrobial resistance and phylogenetic groups in isolates of\n                    <i>Escherichia coli<\/i>\n                    from seagulls at the Berlengas nature reserve"],"prefix":"10.1002","volume":"165","author":[{"given":"H.","family":"Radhouani","sequence":"first","affiliation":[{"name":"Department of Veterinary Science"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Poeta","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Igrejas","sequence":"additional","affiliation":[{"name":"Department of 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