{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T10:43:17Z","timestamp":1761648197266,"version":"build-2065373602"},"reference-count":33,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2010,6,12]],"date-time":"2010-06-12T00:00:00Z","timestamp":1276300800000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:p>\n                    Thirty\u2010five serum samples and six hygroma fluid samples were collected from sexually mature cattle in one herd with clinical signs of brucellosis (abortion and hygromas) in the Western Region of the Gambia in order to isolate and characterise\n                    <jats:italic>Brucella<\/jats:italic>\n                    species\n                    <jats:italic>.<\/jats:italic>\n                    Information on the sex, age, number of calvings, number of abortions, presence of hygromas, and presence of orchitis was also collected for each animal sampled. Twenty\u2010six (74 per cent) of the serum samples were positive in the rose bengal test and 29 (83 per cent) were positive by indirect ELISA. Three isolates of\n                    <jats:italic>Brucella<\/jats:italic>\n                    , biotyped as\n                    <jats:italic>Brucella abortus<\/jats:italic>\n                    biovar 3, were cultured from six hygroma fluid samples. The multiple locus variable number tandem repeat analysis assay clustered the isolates as\n                    <jats:italic>B abortus<\/jats:italic>\n                    with the same profile for the three isolates, suggesting a common origin of contamination.\n                  <\/jats:p>","DOI":"10.1136\/vr.b4862","type":"journal-article","created":{"date-parts":[[2010,10,5]],"date-time":"2010-10-05T17:52:18Z","timestamp":1286301138000},"page":"753-756","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":13,"title":["Phenotypic and genotypic characterisation of\n                    <i>Brucella<\/i>\n                    strains isolated from cattle in the Gambia"],"prefix":"10.1002","volume":"166","author":[{"given":"A. A.","family":"Bankole","sequence":"first","affiliation":[{"name":"Department of Microbiology University Hospital of Antwerp Wilrijkstraat 10 Edegem Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Saegerman","sequence":"additional","affiliation":[{"name":"Department of Infectious and Parasitic Diseases Faculty of Veterinary Medicine Universit\u00e9 de Li\u00e8ge Boulevard de Colonster, 20, B42 Sart\u2010Tilman 4000 Li\u00e8ge Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Berkvens","sequence":"additional","affiliation":[{"name":"Department of Animal Health Institute of Tropical Medicine Nationalestraat 155 2000 Antwerp Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Fretin","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Immunology Veterinary and Agrochemical research Centre Groeselenberg 99 1190 Brussels Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Geerts","sequence":"additional","affiliation":[{"name":"Department of Animal Health Institute of Tropical Medicine Nationalestraat 155 2000 Antwerp Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Ieven","sequence":"additional","affiliation":[{"name":"Department of Microbiology University Hospital of Antwerp Wilrijkstraat 10 Edegem Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Walravens","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Immunology Veterinary and Agrochemical research Centre Groeselenberg 99 1190 Brussels Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,6,12]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"40","volume-title":"Zoonoses and Communicable Diseases Common to Man and Animals. Vol 1: Bacterioses and Mycoses","author":"Acha P. N.","year":"2003"},{"key":"e_1_2_6_3_1","first-page":"97","article-title":"Clinical signs of animal brucellosis in Eastern Sudan","volume":"50","author":"Agab H.","year":"1997","journal-title":"Revue d\u2019Elevage et de M\u00e9decine V\u00e9t\u00e9rinaire des Pays Tropicaux"},{"key":"e_1_2_6_4_1","first-page":"981","article-title":"Epidemiology of animal brucellosis in tropical Africa: clinical, serological, and bacteriological surveys","volume":"6","author":"Akakpo A. J.","year":"1987","journal-title":"Revue Scientifique et Technique \u2013 Office International des Epizooties"},{"key":"e_1_2_6_5_1","first-page":"847","article-title":"La brucellose bovine en Afique tropicale de l\u2019Ouest: \u00e9tats actuels des connaissances","volume":"29","author":"Akakpo A. J.","year":"1982","journal-title":"M\u00e9decine d\u2019Afrique Noire"},{"key":"e_1_2_6_6_1","first-page":"29","volume-title":"Techniques for the Brucellosis Laboratory","author":"Alton G. G.","year":"1988"},{"key":"e_1_2_6_7_1","first-page":"1052","volume-title":"Infectious Diseases of Livestock with Special Reference to Southern Africa","author":"Bishop G. C.","year":"1994"},{"key":"e_1_2_6_8_1","first-page":"829","article-title":"Brucellose animales: sondages s\u00e9rologiques dans quatre pays de l\u2019Afrique de l\u2019Ouest: B\u00e9nin, Cameroun, Haute\u2010Volta et Niger","volume":"29","author":"Bornarel P.","year":"1982","journal-title":"M\u00e9decine d\u2019Afrique Noire"},{"key":"e_1_2_6_9_1","first-page":"262","article-title":"Incidence clinique de la brucellose bovine dans le nord de la C\u00f4ted\u2019Ivoire","volume":"33","author":"Camus E.","year":"1980","journal-title":"Revue d\u2019Elevage et de M\u00e9decine V\u00e9t\u00e9rinaire des Pays Tropicaux"},{"key":"e_1_2_6_10_1","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1111\/j.1751-0813.1985.tb14233.x","article-title":"Use of an enzyme\u2010linked immunoabsorbent assay in a bovine brucellosis eradication programme","volume":"62","author":"Cargil C.","year":"1985","journal-title":"Australian Veterinary Journal"},{"key":"e_1_2_6_11_1","first-page":"833","article-title":"L\u2019infection brucellique aux abattoirs de Dakar. 1. Nouveau sondage s\u00e9rologique sur le personnel.","volume":"131","author":"Chantal J.","year":"1980","journal-title":"Revue de M\u00e9decine V\u00e9t\u00e9rinaire"},{"key":"e_1_2_6_12_1","first-page":"377","volume-title":"Bergey\u2019s Manual of Systematic Bacteriology","author":"Corbel M. J.","year":"1984"},{"key":"e_1_2_6_13_1","first-page":"25","volume-title":"National Livestock Census 1993\u20101994","author":"Department of Livestock Services","year":"1994"},{"key":"e_1_2_6_14_1","first-page":"19","article-title":"La brucellose bovine en Afrique Centrale. VI. Identification et typage des souches isol\u00e9es au Tchad et au Cameroun","volume":"36","author":"Domenech J.","year":"1983","journal-title":"Revue d\u2019Elevage et de M\u00e9decine V\u00e9t\u00e9rinaire des Pays Tropicaux"},{"key":"e_1_2_6_15_1","first-page":"29","article-title":"Isolation of Brucellae from the Nigerian livestock and the typing of such isolates","volume":"26","author":"Eze E. N.","year":"1978","journal-title":"Bulletin of Animal Health and Production in Africa"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-7-315"},{"key":"e_1_2_6_17_1","first-page":"263","article-title":"Brucellose enzootique \u00e0 Brucella suis biovar 2 chez le sanglier (Sus scrofa) en Belgique","volume":"138","author":"Godfroid J.","year":"1994","journal-title":"Annales de M\u00e9decine V\u00e9t\u00e9rinaire"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3156.2004.01306.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-6-9"},{"key":"e_1_2_6_20_1","first-page":"565","article-title":"Le diagnostic s\u00e9rologique de la brucellose bovine par ELISA","volume":"132","author":"Limet J. N.","year":"1988","journal-title":"Annales de M\u00e9decine V\u00e9t\u00e9rinaire"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00249-3"},{"key":"e_1_2_6_22_1","unstructured":"MVLABank(2009)MLVAbank for Bacterial Genotyping:http:\/\/minisatellites.u\u2010psud.fr\/MLVAnet. Accessed September 22 2009"},{"key":"e_1_2_6_23_1","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/S0021-9975(08)80139-9","article-title":"Clinical manifestations of brucellosis in cattle of the southern Darfur Province, Western Sudan","volume":"103","author":"Musa M. T.","year":"1990","journal-title":"Journal of Comparative Pathology"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00229-8"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2004.06.012"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5877(84)90064-3"},{"key":"e_1_2_6_27_1","first-page":"971","volume-title":"Infectious and Parasitic Disease of Livestock","author":"Saegerman C.","year":"2009"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2004.02.003"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.145.8.214"},{"key":"e_1_2_6_30_1","first-page":"104","volume-title":"Animal Health Programme Report","author":"Unger F.","year":"2004"},{"key":"e_1_2_6_31_1","first-page":"16","volume-title":"Animal Health Working Paper 2","author":"Unger F.","year":"2003"},{"key":"e_1_2_6_32_1","first-page":"93","article-title":"La brucellose bovine \u00e0 Brucella melitensis en France","volume":"20","author":"Verger M.","year":"1989","journal-title":"Annales de Recherches V\u00e9t\u00e9rinaires"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02039-05"},{"volume-title":"Current Protocols in Molecular Biology","year":"1990","author":"Wilson K.","key":"e_1_2_6_34_1"}],"container-title":["Veterinary Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1136%2Fvr.b4862","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1136\/vr.b4862","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T10:36:14Z","timestamp":1761647774000},"score":1,"resource":{"primary":{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/10.1136\/vr.b4862"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,6]]},"references-count":33,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2010,6]]}},"alternative-id":["10.1136\/vr.b4862"],"URL":"https:\/\/doi.org\/10.1136\/vr.b4862","archive":["Portico"],"relation":{},"ISSN":["0042-4900","2042-7670"],"issn-type":[{"type":"print","value":"0042-4900"},{"type":"electronic","value":"2042-7670"}],"subject":[],"published":{"date-parts":[[2010,6]]}}}