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Viral loads were determined by real\u2010time quantitative RT\u2010PCR in the liver, spleen, cervical lymph node, mandibular lymph node, retropharyngeal lymph node, soft palate, pharynx, tonsil, tongue and skin (coronary band area). Tissue samples were collected from both inoculated and contact\u2010infected pigs at several time points during infection, and blood samples were collected to assess viraemia and its relationship to tissue viral load. Virus first appeared in the lymph nodes, followed by viraemia and then clinical signs. The results suggested that FMDV accumulated in lymphoid tissue up to six hours after infection, in the tissues drained by the mandibular lymph node and tonsil and then disseminated throughout the body where epithelial cells were the favoured sites of replication.<\/jats:p>","DOI":"10.1136\/vr.b5583","type":"journal-article","created":{"date-parts":[[2010,10,5]],"date-time":"2010-10-05T17:52:18Z","timestamp":1286301138000},"page":"10-14","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":27,"title":["Foot\u2010and\u2010mouth disease viral loads in pigs in the early, acute stage of disease"],"prefix":"10.1002","volume":"166","author":[{"given":"C.","family":"Murphy","sequence":"first","affiliation":[{"name":"Institute for Animal Health Pirbright Laboratory Ash Road, Pirbright, Woking Surrey GU24 0NF"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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