{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T16:13:29Z","timestamp":1791562409222,"version":"4.3.4"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2010,7,17]],"date-time":"2010-07-17T00:00:00Z","timestamp":1279324800000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:p>\n                    The performance of a serum PCR assay was compared with that of a blood PCR assay for the diagnosis of canine brucellosis caused by\n                    <jats:italic>Brucella canis<\/jats:italic>\n                    in 72 dogs. The dogs were classified into three groups (infected, non\u2010infected and suspected brucellosis) according to the results of blood culture and serological tests. The sensitivities of blood PCR and serum PCR were, respectively, 97.14 per cent and 25.71 per cent. The specificities of both were 100 per cent. In the group of dogs with suspected brucellosis, three were positive by blood PCR and none was positive by serum PCR. Serum PCR showed little value for the direct diagnosis of canine brucellosis as the assay had low diagnostic sensitivity and fewer positive dogs were detected by this test than by blood culture, blood PCR, rapid slide agglutination test (RSAT) and RSAT with 2\u2010mercaptoethanol.\n                  <\/jats:p>","DOI":"10.1136\/vr.c3811","type":"journal-article","created":{"date-parts":[[2010,10,5]],"date-time":"2010-10-05T17:52:18Z","timestamp":1286301138000},"page":"96-99","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":16,"title":["Comparison of a PCR assay in whole blood and serum specimens for canine brucellosis diagnosis"],"prefix":"10.1002","volume":"167","author":[{"given":"L. B.","family":"Keid","sequence":"first","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade de S\u00e3o Paulo Rua Prof Dr Orlando Marques de Paiva, 87 05508\u2010900 S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. M.","family":"Soares","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade de S\u00e3o Paulo Rua Prof Dr Orlando Marques de Paiva, 87 05508\u2010900 S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. A.","family":"Vasconcellos","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade de S\u00e3o Paulo Rua Prof Dr Orlando Marques de Paiva, 87 05508\u2010900 S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V. R.","family":"Salgado","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade de S\u00e3o Paulo Rua Prof Dr Orlando Marques de Paiva, 87 05508\u2010900 S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Megid","sequence":"additional","affiliation":[{"name":"Departamento de Higiene Veterin\u00e1ria e Sa\u00fade P\u00fablica Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade Estadual Paulista Botucatu Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. J.","family":"Richtzenhain","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade de S\u00e3o Paulo Rua Prof Dr Orlando Marques de Paiva, 87 05508\u2010900 S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,7,17]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"175","volume-title":"Las tecnicas de laboratorios en la brucellosis","author":"Alton G. G.","year":"1976"},{"key":"e_1_2_6_3_1","first-page":"271","article-title":"Detection of Brucella melitensis and Brucella abortus by DNA amplification","volume":"95","author":"Baily G. G.","year":"1992","journal-title":"Journal of Tropical Medicine and Hygiene"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00228-6"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.diagmicrobio.2005.03.011","article-title":"Detection of Brucella DNA in sera from patients with brucellosis by polymerase chain reaction","volume":"53","author":"Elfaki M. G.","year":"2005","journal-title":"Diagnostic and Microbiology of Infectious Diseases"},{"key":"e_1_2_6_6_1","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/j.theriogenology.2006.04.011","article-title":"Canine brucellosis: outbreaks and compliance","volume":"66","author":"Holett R. B.","year":"2006","journal-title":"Theriogenology"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2007.01.003"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2007.08.021"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2008.05.012"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-006-0109-6"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.68.615"},{"key":"e_1_2_6_12_1","doi-asserted-by":"crossref","first-page":"3087","DOI":"10.1128\/jcm.33.12.3087-3090.1995","article-title":"Single\u2010step PCR for detection of Brucella spp from blood and milk of infected animals","volume":"33","author":"Leal \u2010Klevezas D. S.","year":"1995","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_6_13_1","doi-asserted-by":"crossref","first-page":"2443","DOI":"10.1128\/JCM.36.9.2443-2446.1998","article-title":"Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis","volume":"36","author":"Morata P.","year":"1998","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.4.1.115"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2002.tb00616.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00270-07"},{"key":"e_1_2_6_17_1","doi-asserted-by":"crossref","first-page":"248","DOI":"10.2144\/99272bm06","article-title":"Hydrogen peroxide improves the efficiency of a peripheral blood PCR assay for diagnosis of human brucellosis","volume":"27","author":"Queipo\u2010Ortu\u00f1o M. I.","year":"1999","journal-title":"Biotechniques"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-007-0409-y"},{"key":"e_1_2_6_19_1","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.anireprosci.2004.05.005","article-title":"Canine brucellosis","volume":"82","author":"Wanke M. M.","year":"2004","journal-title":"Animal Reproduction Science"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.11.3437-3442.1999"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.4.1661-1664.2001"}],"container-title":["Veterinary Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1136%2Fvr.c3811","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1136\/vr.c3811","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T11:24:17Z","timestamp":1761650657000},"score":1,"resource":{"primary":{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/10.1136\/vr.c3811"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,7]]},"references-count":20,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2010,7]]}},"alternative-id":["10.1136\/vr.c3811"],"URL":"https:\/\/doi.org\/10.1136\/vr.c3811","archive":["Portico"],"relation":{},"ISSN":["0042-4900","2042-7670"],"issn-type":[{"value":"0042-4900","type":"print"},{"value":"2042-7670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,7]]}}}