{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T04:12:46Z","timestamp":1768450366595,"version":"3.49.0"},"reference-count":18,"publisher":"Wiley","issue":"25","license":[{"start":{"date-parts":[[2010,12,18]],"date-time":"2010-12-18T00:00:00Z","timestamp":1292630400000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:p>\n                    A real\u2010time PCR was developed to detect\n                    <jats:italic>Coxiella burnetii<\/jats:italic>\n                    (the cause of Q fever) in ruminant placentas and aborted fetuses. Primer and probe sets previously developed for human tissue studies were used to target the insertion sequence IS\n                    <jats:italic>1111<\/jats:italic>\n                    gene for\n                    <jats:italic>C burnetii<\/jats:italic>\n                    . The assay was highly sensitive, with a limit of detection of 10 copies of template, theoretically equating to a single bacterium, and did not cross\u2010react with a panel of other bacteria. To determine sensitivity on field samples submitted for the diagnosis of abortion, results using the IS\n                    <jats:italic>1111<\/jats:italic>\n                    PCR assay were compared with a\n                    <jats:italic>com<\/jats:italic>\n                    1\u00a0PCR assay. When applied to ruminant abortion material, including placental cotyledons and fetal samples, the IS\n                    <jats:italic>1111<\/jats:italic>\n                    and\n                    <jats:italic>com<\/jats:italic>\n                    1 assays yielded positive results in 23 (25 per cent) of 93 and 19 (20 per cent) of 93 samples, respectively. One infected goat herd was monitored for 31\u00a0months: 57 (92 per cent) of 62 placental cotyledon samples from aborting and non\u2010aborting goats, and 10 (30 per cent) of 33 fetal samples were positive by the IS\n                    <jats:italic>1111<\/jats:italic>\n                    PCR assay.\n                  <\/jats:p>","DOI":"10.1136\/vr.c4040","type":"journal-article","created":{"date-parts":[[2010,12,17]],"date-time":"2010-12-17T09:28:24Z","timestamp":1292578104000},"page":"965-967","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":23,"title":["Detection of\n                    <i>Coxiella burnetii<\/i>\n                    in placenta and abortion samples from British ruminants using real\u2010time PCR"],"prefix":"10.1002","volume":"167","author":[{"given":"R. M.","family":"Jones","sequence":"first","affiliation":[{"name":"Veterinary Laboratories Agency (VLA) \u2013 Weybridge Woodham Lane, New Haw Addlestone Surrey KT15 3NB"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. F.","family":"Twomey","sequence":"additional","affiliation":[{"name":"VLA \u2013 Starcross Staplake Mount, Starcross Exeter Devon EX6 8PE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Hannon","sequence":"additional","affiliation":[{"name":"VLA \u2013 Penrith, Merrythought, Calthwaite Penrith Cumbria CA11 9RR"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Errington","sequence":"additional","affiliation":[{"name":"VLA \u2013 Penrith, Merrythought, Calthwaite Penrith Cumbria CA11 9RR"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. C.","family":"Pritchard","sequence":"additional","affiliation":[{"name":"VLA \u2013 Bury St Edmunds Rougham Hill Bury St Edmunds Suffolk IP33 2RX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Sawyer","sequence":"additional","affiliation":[{"name":"Veterinary Laboratories Agency (VLA) \u2013 Weybridge Woodham Lane, New Haw Addlestone Surrey KT15 3NB"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,12,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.07.016"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2005010"},{"key":"e_1_2_6_4_1","first-page":"153","article-title":"PCR\u2010based detection of Coxiella burnetii from clinical samples.","volume":"216","author":"Berri M.","year":"2003","journal-title":"Methods in Molecular Biology"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(99)00178-9"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2006.11.001"},{"key":"e_1_2_6_7_1","first-page":"365","article-title":"Q fever in the Netherlands: a concise overview and implications of the largest ongoing outbreak.","volume":"66","author":"Delsing C. 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