{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T11:41:47Z","timestamp":1777462907634,"version":"3.51.4"},"reference-count":31,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2011,4,23]],"date-time":"2011-04-23T00:00:00Z","timestamp":1303516800000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2011,4]]},"abstract":"<jats:p>\n                    The aim of this study was to detect\n                    <jats:italic>Brucella<\/jats:italic>\n                    in samples from aborted fetuses of sheep and cattle in Turkey using PCR and bacteriological analysis, and to determine the sensitivity and specificity of the PCR. Organ homogenates from 38 aborted fetuses of cattle and 56 aborted fetuses of sheep were tested. All organ homogenateswere cultured for bacteriological analysis, and all of the homogenates and the Brucella isolates obtained by culture were examined with a commercial PCR kit. On bacteriological analysis,\n                    <jats:italic>Brucella<\/jats:italic>\n                    species were found in 30 (31.9 per cent) of the 94 organ homogenates, eight (21.1 per cent) of which were from cattle and 22 (39.3 per cent) from sheep. Using PCR, a total of 29 (30.9 per cent) homogenates were positive for\n                    <jats:italic>Brucella<\/jats:italic>\n                    species, eight (21.1 per cent) of which were from cattle and 21 (37.5 per cent) from sheep. Compared with the bacteriological method, the diagnostic sensitivity and specificity of the PCR kit used in this study were 83 per cent and 94 per cent, respectively.\n                  <\/jats:p>","DOI":"10.1136\/vr.c7003","type":"journal-article","created":{"date-parts":[[2011,4,14]],"date-time":"2011-04-14T20:37:41Z","timestamp":1302813461000},"page":"430-430","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":8,"title":["Comparison of direct culture versus PCR for the detection of\n                    <i>Brucella<\/i>\n                    in aborted fetuses of cattle and sheep in Turkey"],"prefix":"10.1002","volume":"168","author":[{"given":"E.","family":"Buyukcangaz","sequence":"first","affiliation":[{"name":"Department of Microbiology Faculty of Veterinary Medicine Uludag University, Gorukle Bursa 16059 Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Sen","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Veterinary Medicine Uludag University, Gorukle Bursa 16059 Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. 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