{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:09:36Z","timestamp":1791266976588,"version":"4.1.0"},"reference-count":17,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2011,4,30]],"date-time":"2011-04-30T00:00:00Z","timestamp":1304121600000},"content-version":"vor","delay-in-days":29,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2011,4]]},"abstract":"<jats:p>\n                    This study was undertaken to evaluate two monoclonal antibody\u2010based sandwich ELISAs (sELISAs) for the detection of\n                    <jats:italic>Clostridium botulinum<\/jats:italic>\n                    neurotoxins (BoNTs) types C and D from culture\u2010enriched intestinal content samples from cattle. To validate the diagnostic significance of the presence of cultivable, toxin\u2010producing\n                    <jats:italic>C botulinum<\/jats:italic>\n                    in the intestines of cattle, samples from both suspect and non\u2010suspect botulism cases were examined. BoNT was detected by both sELISAs in a greater number of suspect animals than by direct testing of uncultured samples by mouse bioassay. One sELISA detected two BoNT C and one BoNT Group III mosaic isoform in three animals that were missed by the other, and both sELISAs failed to identify samples from two mouse bioassay\u2010positive BoNT C animals. BoNT D was also detected in one non\u2010suspect sample by one of the sELISAs.\n                  <\/jats:p>","DOI":"10.1136\/vr.c7432","type":"journal-article","created":{"date-parts":[[2011,4,11]],"date-time":"2011-04-11T22:14:57Z","timestamp":1302560097000},"page":"455-455","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":19,"title":["Diagnosis of botulism types C and D in cattle by a monoclonal antibody\u2010based sandwich ELISA"],"prefix":"10.1002","volume":"168","author":[{"given":"C. E.","family":"Brooks","sequence":"first","affiliation":[{"name":"Veterinary Sciences Division Agri\u2010Food and Biosciences Institute Northern Ireland Stoney Road, Stormont Belfast BT4 3SD"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. J.","family":"Clarke","sequence":"additional","affiliation":[{"name":"Veterinary Sciences Division Agri\u2010Food and Biosciences Institute Northern Ireland Stoney Road, Stormont Belfast BT4 3SD"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. A.","family":"Graham","sequence":"additional","affiliation":[{"name":"Veterinary Sciences Division Agri\u2010Food and Biosciences Institute Northern Ireland Stoney Road, Stormont Belfast BT4 3SD"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. J.","family":"Ball","sequence":"additional","affiliation":[{"name":"Veterinary Sciences Division Agri\u2010Food and Biosciences Institute Northern Ireland Stoney Road, Stormont Belfast BT4 3SD"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,4,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.308.6943.1552"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.87.9.258"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.12.030"},{"key":"e_1_2_6_5_1","unstructured":"CDC(1998) Botulism in the United States 1899\u20101996. Handbook for Epidemiologists Clinicians and Laboratory Workers. Centers for Disease Control and Prevention"},{"key":"e_1_2_6_6_1","volume-title":"Clostridia: The Impact of Genomics on Disease Control. 6th ClostPath International Conference","author":"Clarke H. 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Novel insights into the receptor for clostridial neurotoxins.","volume":"280","author":"Tsukamoto K.","year":"2005","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063879500700131"}],"container-title":["Veterinary Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1136%2Fvr.c7432","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1136\/vr.c7432","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T11:41:48Z","timestamp":1761651708000},"score":1,"resource":{"primary":{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/10.1136\/vr.c7432"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,4]]},"references-count":17,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2011,4]]}},"alternative-id":["10.1136\/vr.c7432"],"URL":"https:\/\/doi.org\/10.1136\/vr.c7432","archive":["Portico"],"relation":{},"ISSN":["0042-4900","2042-7670"],"issn-type":[{"value":"0042-4900","type":"print"},{"value":"2042-7670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,4]]}}}