{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:23:21Z","timestamp":1773289401616,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2011,11,5]],"date-time":"2011-11-05T00:00:00Z","timestamp":1320451200000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:p>The sensitivity and specificity of three commercially available complement fixation test (CFT) antigens from c.c.pro (c.c.pro), Central Veterinary Institute of Wageningen UR (CIDC) and the United States Department of Agriculture (USDA) were comparatively evaluated by testing 410 sera collected from glanders\u2010endemic and non\u2010endemic areas (200 true\u2010negative randomly collected sera and 210 sera collected from experimentally immunised animals (12 rabbits, 19 horses), clinically positive (135) and culture\u2010positive (44) horses, donkeys and mules). Immunoblotting (IB) was used as the gold standard test. Highest sensitivity was shown for the CIDC antigen (100 per cent) followed by the c.c.pro antigen (99.39 per cent). However, the USDA antigen showed substantially less (p&lt;0.05) sensitivity (62.19 per cent). Highest specificity was found for the USDA antigen (100 per cent) followed by the CIDC (97.5 per cent) and c.c.pro antigen (96.5 per cent). Positive and negative predictive values (assumed glanders prevalence of &lt;0.1 per cent) for each antigen were calculated to be 95.88 and 99.48 (c.c.pro), 97.04 and 100 (CIDC), 100 and 76.33 per cent (USDA), respectively. Almost perfect agreement (0.96) was found between CFT using either c.c.pro or CIDC and IB.<\/jats:p>","DOI":"10.1136\/vr.d5410","type":"journal-article","created":{"date-parts":[[2011,9,6]],"date-time":"2011-09-06T20:37:13Z","timestamp":1315341433000},"page":"495-495","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":22,"title":["Comparative evaluation of three commercially available complement fixation test antigens for the diagnosis of glanders"],"prefix":"10.1002","volume":"169","author":[{"given":"I.","family":"Khan","sequence":"first","affiliation":[{"name":"Friedrich\u2010Loeffler\u2010Institute Federal Research Institute for Animal Health Institute of Bacterial Infections and Zoonoses Naumburger Strasse 96a 07743 Jena Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. 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A.","family":"Santana","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Nacional Agropecu\u00e1rio Unidade de Diagn\u00f3stico Bacteriol\u00f3gico Minist\u00e9rio da Agricultura Pecu\u00e1ria e Abastecimento Rua Dom Manoel De Medeiros, S\/N Dois Irm\u00e3os Recife PE CEP 52171\u2010030 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. M. A.","family":"de Souza","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Nacional Agropecu\u00e1rio Unidade de Diagn\u00f3stico Bacteriol\u00f3gico Minist\u00e9rio da Agricultura Pecu\u00e1ria e Abastecimento Rua Dom Manoel De Medeiros, S\/N Dois Irm\u00e3os Recife PE CEP 52171\u2010030 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Saqib","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine and Surgery University of Agriculture Faisalabad 38040 Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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