{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T16:35:43Z","timestamp":1770482143683,"version":"3.49.0"},"reference-count":32,"publisher":"BMJ","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1975,9]]},"abstract":"<jats:p>The morphology of epithelial lymphocytes in osmium-fixed, Epon-embedded jejunum of adult mice was studied by light and electron microscopy. Toluidine blue-stained 1 mum 'thick' plastic sections were compared with adjacent thin sections, thereby permitting precise ultrastructural identification and description of selected epithelial lymphocytes. Their size and appearances varied considerably, ranging from typical small lymphocytes through medium-sized lymphocytes to large immunoblasts. A high proportion of medium-sized epithelial lymphocytes (mean diameter 6-9 +\/- 1-1 mum) contained several lysosomes, extensive Golgi complexes, prominent centrioles and abundant ribosomes. Their appearances, therefore, corresponded directly to mitogen-stimulated lymphocytes. In contrast, immunoblasts were big cells (mean diameter 11-0 +\/- 0-8 mum) with large, euchromatic nuclei and prominent nucleoli. The majority had pale-staining, ribosome-studded cytoplasm and thus resembled type I, or T blasts. Very rarely, densely staining blasts containing ribosomes and well developed rough endoplasmic reticulum were observed; these corresponded to type II or B blasts. These observations indicate that transformation of lymphocytes occurs within the interepithelial cell spaces of the small intestinal mucosa, suggesting that epithelial lymphocytes are immunocompetent cells which may be responsive to local antigenic stimulation.<\/jats:p>","DOI":"10.1136\/gut.16.9.665","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"665-674","source":"Crossref","is-referenced-by-count":41,"title":["Studies of intestinal lymphoid tissue. I. Electron microscopic evidence of 'blast transformation' in epithelial lymphocytes of mouse small intestinal mucosa."],"prefix":"10.1136","volume":"16","author":[{"given":"M N","family":"Marsh","sequence":"first","affiliation":[]}],"member":"239","published-online":{"date-parts":[[1975,9,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1093\/jnci\/35.1.113","article-title":"Lymphocyte transformation in epithelium","volume":"35","author":"Andrew, W.","year":"1965","unstructured":"Andrew, W. (1965). Lymphocyte transformation in epithelium. J. nat. Cancer Inst., 35, 113-137.","journal-title":"J. nat. Cancer Inst"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1002\/ar.1090970108","article-title":"Mitotic division and degeneration of lymphocytes within cells of intestinal epithelium in young and in adult white mice","volume":"97","author":"Andrew, W.; Sosa, J.M.","year":"1947","unstructured":"Andrew, W., and Sosa, J. M. (1947). Mitotic division and degeneration of lymphocytes within cells of intestinal epithelium in young and in adult white mice. Anat. Rec. 97, 63-97.","journal-title":"Anat. Rec"},{"key":"ref_3","first-page":"1040","article-title":"Ultrastructure of lymphoid and plasma cells in relation to globulin and antibody formation","volume":"10","author":"Bessis, M.C.","year":"1961","unstructured":"Bessis, M. C. (1961). Ultrastructure of lymphoid and plasma cells in relation to globulin and antibody formation. Lab. Invest., 10, 1040-1067.","journal-title":"Lab. Invest"},{"key":"ref_4","first-page":"1","article-title":"Morphogenesis in blood lymphocytes stimulated with phytohaemagglutinin (PHA)","volume":"223","author":"Biberfeld, P.","year":"1971","unstructured":"Biberfeld, P. (1971a). Morphogenesis in blood lymphocytes stimulated with phytohaemagglutinin (PHA). Acta path. microbiol. scand., A. Suppl. 223, 1.","journal-title":"Acta path. microbiol. scand., A"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S0022-5320(71)80040-0","article-title":"Endocytosis and lysosome formation in blood lymphocytes transformed by phytohemagglutinin","volume":"37","author":"Biberfeld, P.","year":"1971","unstructured":"Biberfeld, P. (1971b). Endocytosis and lysosome formation in blood lymphocytes transformed by phytohemagglutinin. J. ultrastruct. Res., 37, 41-68.","journal-title":"J. ultrastruct. Res"},{"key":"ref_6","first-page":"64","article-title":"Electron microscopy of the vacuolar apparatus","volume-title":"Lysosomes in Biology and Pathology","volume":"1","author":"Daems, W.T.; Wisse, E.; Brederoo, P.","year":"1969","unstructured":"Daems, W. T., Wisse, E., and Brederoo, P. (1969). Electron microscopy of the vacuolar apparatus. In Lysosomes in Biology and Pathology, edited by J. T. Dingle and H. B. Fell, vol. 1, p. 64. North-Holland, Amsterdam."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1038\/2071405a0","article-title":"Immunological significance of lysosomes within lymphocytes in vivo","volume":"207","author":"Diengdoh, J.V.; Turk, J.L.","year":"1965","unstructured":"Diengdoh, J. V., and Turk, J. L. (1965). Immunological significance of lysosomes within lymphocytes in vivo. Nature (Lond.), 207, 1405-1406.","journal-title":"Nature (Lond.)"},{"key":"ref_8","first-page":"17","article-title":"Studies on human peripheral blood lymphocytes in vitro: III. Fine structural features of lymphocyte transformation of pokeweed mitogen","volume":"98","author":"Douglas, S.D.; Hoffman, P.F.; Borjeson, J.; Chessin, L.N.","year":"1967","unstructured":"Douglas, S. D., Hoffman, P. F., Borjeson, J., and Chessin, L. N. (1967). Studies on human peripheral blood lymphocytes in vitro: III. Fine structural features of lymphocyte transformation of pokeweed mitogen. J. Immunol., 98, 17-30.","journal-title":"J. Immunol"},{"key":"ref_9","first-page":"41","article-title":"Human lymphocyte growth in vitro: morphologic, biochemical and immunologic significance","volume":"10","author":"Douglas, S.D.","year":"1971","unstructured":"Douglas, S. D. (1971). Human lymphocyte growth in vitro: morphologic, biochemical and immunologic significance. Int. Rev. exp. Path., 10, 41-114.","journal-title":"Int. Rev. exp. Path"},{"key":"ref_10","first-page":"39","article-title":"Electron microscopic and functional aspects of human lymphocyte response to mitogens","volume":"11","author":"Douglas, S.D.","year":"1972","unstructured":"Douglas, S. D. (1972). Electron microscopic and functional aspects of human lymphocyte response to mitogens. Trans. Rev., 11, 39-59.","journal-title":"Trans. Rev"},{"key":"ref_11","first-page":"477","article-title":"The effect of antigen deprivation of thymus-dependent and thymus-independent lymphocytes in the small intestine of the mouse","volume":"12","author":"Ferguson, A.; Parrott, D.M.V.","year":"1972","unstructured":"Ferguson, A., and Parrott, D. M. V. (1972). The effect of antigen deprivation of thymus-dependent and thymus-independent lymphocytes in the small intestine of the mouse. Clin. exp. Immunol., 12, 477-488.","journal-title":"Clin. exp. Immunol"},{"key":"ref_12","first-page":"87","article-title":"Elicitation of selective T and B lymphocyte responses by cell surface binding ligands","volume":"11","author":"Greaves, M.; Janossy, G.","year":"1972","unstructured":"Greaves, M., and Janossy, G. (1972). Elicitation of selective T and B lymphocyte responses by cell surface binding ligands. Transplant. Rev., 11, 87-130.","journal-title":"Transplant. Rev"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1084\/jem.132.3.448","article-title":"Studies on antibody-producing cells. I. Ultrastructure of 19S and 7S antibody-producing cells","volume":"132","author":"Gudat, F.G.; Harris, T.N.; Harris, S.; Hummeler, K.","year":"1970","unstructured":"Gudat, F. G., Harris, T. N., Harris, S., and Hummeler, K. (1970). Studies on antibody-producing cells. I. Ultrastructure of 19S and 7S antibody-producing cells. J. exp. Med., 132, 448-474.","journal-title":"J. exp. Med"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1002\/eji.1830040610","article-title":"The gut-associated lymphoid system: nature and properties of the large dividing cells","volume":"4","author":"Guy-Grand, D.; Griscelli, C.; Vassali, P.","year":"1974","unstructured":"Guy-Grand, D., Griscelli, C., and Vassali, P. (1974). The gut-associated lymphoid system: nature and properties of the large dividing cells. Europ. J. Immunol., 4, 435-443.","journal-title":"Europ. J. Immunol"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1083\/jcb.37.2.412","article-title":"Studies on lysosomes. XII. Redistribution of acid hydrolases in human lymphocytes stimulated by phytohaemagglutinin","volume":"37","author":"Hirschhorn, R.; Brittinger, G.; Hirschhorn, K.; Weissmann, G.","year":"1968","unstructured":"Hirschhorn, R., Brittinger, G., Hirschhorn, K., and Weissmann, G. (1968). Studies on lysosomes. XII. Redistribution of acid hydrolases in human lymphocytes stimulated by phytohaemagglutinin. J. Cell Biol., 37, 412-423.","journal-title":"J. Cell Biol"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1084\/jem.135.3.491","article-title":"Studies on antibody-producing cells. IV. Ultrastructure of plaque-forming cells of rabbit lymph","volume":"135","author":"Hummeler, K.; Harris, T.N.; Harris, S.; Farber, M.B.","year":"1972","unstructured":"Hummeler, K., Harris, T. N., Harris, S., and Farber, M. B. (1972). Studies on antibody-producing cells. IV. Ultrastructure of plaque-forming cells of rabbit lymph. J. exp. Med., 135, 491-502.","journal-title":"J. exp. Med"},{"key":"ref_17","first-page":"211","article-title":"Lymphocyte activation. IV. The ultrastructural pattern of the response of mouse T and B cells to mitogenic stimulation in vivo","volume":"24","author":"Janossy, G.; Shohat, M.; Greaves, M.F.; Dourmashkin, R.(1973","unstructured":"Janossy, G., Shohat, M., Greaves, M. F., and Dourmashkin, R.(1973). Lymphocyte activation. IV. The ultrastructural pattern of the response of mouse T and B cells to mitogenic stimulation in vivo. Immunology, 24, 211-227.","journal-title":"Immunology"},{"key":"ref_18","first-page":"565","article-title":"The transformation in vitro of peripheral lymphocytes of some laboratory animals","volume":"9","author":"Knight, S.; Ling, N.R.; Sell, S.; Oxnard, C.E.","year":"1965","unstructured":"Knight, S., Ling, N. R., Sell, S., and Oxnard, C. E. (1965). The transformation in vitro of peripheral lymphocytes of some laboratory animals. Immunology, 9, 565-574.","journal-title":"Immunology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1002\/aja.1001210318","article-title":"Electron and light microscopic observations on relationships between lymphocytes and intestinal epithelium","volume":"121","author":"Meader, R.D.; Landers, D.F.","year":"1967","unstructured":"Meader, R. D., and Landers, D. F. (1967). Electron and light microscopic observations on relationships between lymphocytes and intestinal epithelium. Amer. J. Anat., 121, 763-774.","journal-title":"Amer. J. Anat"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/0014-4800(65)90031-6","article-title":"The fine structure of the lymphoid tisssue during antibody formation","volume":"4","author":"Movat, H.Z.; Fernando, N.V.P.","year":"1965","unstructured":"Movat, H. Z., and Fernando, N. V. P. (1965). The fine structure of the lymphoid tisssue during antibody formation. Exp. molec. Path., 4, 155-188.","journal-title":"Exp. molec. Path"},{"key":"ref_21","first-page":"1736","article-title":"The morphologic and cytochemical demonstration of lysosomes in lymphocytes incubated with phytohemagglutinin by electron microscopy","volume":"14","author":"Parker, J.W.; Wakasa, H.; Lukes, R.J.","year":"1965","unstructured":"Parker, J. W., Wakasa, H., and Lukes, R. J. (1965). The morphologic and cytochemical demonstration of lysosomes in lymphocytes incubated with phytohemagglutinin by electron microscopy. Lab. Invest., 14, 1736-1743.","journal-title":"Lab. Invest"},{"key":"ref_22","first-page":"571","article-title":"Selective migration of lymphocytes within the mouse small intestine","volume":"26","author":"Parrott, D.M.V.; Ferguson, A.","year":"1974","unstructured":"Parrott, D. M. V., and Ferguson, A. (1974). Selective migration of lymphocytes within the mouse small intestine. Immunology, 26, 571-588.","journal-title":"Immunology"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1083\/jcb.26.3.759","article-title":"Localization of antibodies by electron microscopy in developing antibody-producing cells","volume":"26","author":"de Petris, S.; Karlsbad, G.","year":"1965","unstructured":"de Petris, S., and Karlsbad, G. (1965). Localization of antibodies by electron microscopy in developing antibody-producing cells. J. Cell Biol., 26, 759-778.","journal-title":"J. Cell Biol"},{"key":"ref_24","first-page":"260","article-title":"Isolation and characteristics of gut mucosal lymphocytes","volume":"30","author":"Rudzik, O.; Bienenstock, J.","year":"1974","unstructured":"Rudzik, O., and Bienenstock, J. (1974). Isolation and characteristics of gut mucosal lymphocytes. Lab. Invest., 30, 260-266.","journal-title":"Lab. Invest"},{"key":"ref_25","first-page":"92","volume-title":"Leukocyten in Epithel. In Handbuch der mikrpischen Anatomie des Menschen","author":"Schaffer, J.","year":"1936","unstructured":"Schaffer, J. (1936). Leukocyten in Epithel. In Handbuch der mikrpischen Anatomie des Menschen, edited by von Mollendorf, p. 92. Springer, Berlin."},{"key":"ref_26","first-page":"129","article-title":"Quantitative studies on small lymphocyte disposition in epithelial cells","volume":"54","author":"Shields, J.W.; Touchon, R.C.; Dickson, D.R.","year":"1969","unstructured":"Shields, J. W., Touchon, R. C., and Dickson, D. R. (1969). Quantitative studies on small lymphocyte disposition in epithelial cells. Amer. J. Path., 54, 129-145.","journal-title":"Amer. J. Path"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1084\/jem.131.1.77","article-title":"Specific antibody within lymphoid germinal center cells of mice after primary immunization with horseradish peroxidase. A light and electron microscopic study","volume":"131","author":"Sordat, B.; Sordat, M.; Hess, M.W.; Stoner, R.D.; Cottier, H.","year":"1970","unstructured":"Sordat, B., Sordat, M., Hess, M. W., Stoner, R. D., and Cottier, H. (1970). Specific antibody within lymphoid germinal center cells of mice after primary immunization with horseradish peroxidase. A light and electron microscopic study. J. exp. Med., 131, 77-91.","journal-title":"J. exp. Med"},{"key":"ref_28","first-page":"69","article-title":"Antibody-carrying cells in the immune response II. Ultrastructure of 'rosette'-forming cells","volume":"4","author":"Storb, U.; Chambers, V.; Storb, R.; Weiser, R.S.","year":"1967","unstructured":"Storb, U., Chambers, V., Storb, R., and Weiser, R. S. (1967). Antibody-carrying cells in the immune response II. Ultrastructure of 'rosette'-forming cells. J. reticuloendothel. Soc., 4, 69-83.","journal-title":"J. reticuloendothel. Soc"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/S0022-5320(71)80076-X","article-title":"Intraepithelial cells in the human intestinal mucosa","volume":"34","author":"Toner, P.G.; Ferguson, A.","year":"1971","unstructured":"Toner, P. G., and Ferguson, A. (1971). Intraepithelial cells in the human intestinal mucosa. J. ultrastruct. Res., 34, 329-344.","journal-title":"J. ultrastruct. Res"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1113\/expphysiol.1966.sp001850","article-title":"Ultrastructural changes in lymphocytes exposed to noxious agents in vitro","volume":"51","author":"Trowell, 0. A","year":"1966","unstructured":"Trowell, 0. A. (1966). Ultrastructural changes in lymphocytes exposed to noxious agents in vitro. Quart. J. exp. Physiol., 51, 207-220.","journal-title":"Quart. J. exp. Physiol"},{"key":"ref_31","first-page":"1159","volume-title":"Biochemistry and function of lysosomes. In Handbook of Molecular Cytology","author":"Wattiaux, R.","year":"1969","unstructured":"Wattiaux, R. (1969). Biochemistry and function of lysosomes. In Handbook of Molecular Cytology, edited by A. Lima-de-Fariau, p. 1159. North-Holland, Amsterdam."},{"key":"ref_32","first-page":"90","article-title":"Zur Frage der Lymphocytenwanderung durch das Darmepithel","volume":"45","author":"Wolf-Heidegger, G.","year":"1939","unstructured":"Wolf-Heidegger, G. (1939). Zur Frage der Lymphocytenwanderung durch das Darmepithel. Z. mikr.-anat. Forsch., 45, 90-103.","journal-title":"Z. mikr.-anat. 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