{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T23:22:55Z","timestamp":1780096975551,"version":"3.54.0"},"reference-count":17,"publisher":"BMJ","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"accepted":{"date-parts":[[1998,1,19]]},"published-print":{"date-parts":[[1998,6,1]]},"abstract":"<jats:p>\n                  <jats:italic>Background<\/jats:italic>\u2014The peptide hormone gastrin is a recognised growth factor for gastrointestinal (GI) tumour cells. Carboxyamidated gastrins bind to the cell surface gastrin\/cholecystokinin B (CCK-B) receptor which can be expressed as either a normal or a truncated isoform (\u0394CCK-B).<\/jats:p>\n               <jats:p>\n                  <jats:italic>Aims<\/jats:italic>\u2014To compare gastrin gene expression with \u0394CCK-B and total CCK-B (both isoforms) gene expression in both GI and non-GI tract derived human tumour cell lines.<\/jats:p>\n               <jats:p>\n                  <jats:italic>Methods<\/jats:italic>\u2014Total RNA was extracted and gene expression was assayed by the reverse transcription-polymerase chain reaction followed by Southern blotting and hybridisation with specific oligo probes.<\/jats:p>\n               <jats:p>\n                  <jats:italic>Results<\/jats:italic>\u2014Gastrin was expressed by 5\/5 gastric and 7\/8 colorectal cell lines. Coexpression of gastrin CCK-B isoform was found in 80% of gastric and 75% of colorectal cell lines. Non-GI cell lines, with the exception of a lymphoblastic leukaemia cell line, showed no coexpression. The truncated receptor, \u0394CCK-B, was shown in 3\/5 gastric and 5\/8 colorectal cell lines and was always coexpressed with gastrin.<\/jats:p>\n               <jats:p>\n                  <jats:italic>Conclusions<\/jats:italic>\u2014The truncated gastrin receptor, \u0394CCK-B, is coexpressed with gastrin in 8\/13 GI tumour cell lines. Gastrin and CCK-B receptor isoforms may be involved in maintaining autocrine\/paracrine growth pathways in GI cancer cells.<\/jats:p>","DOI":"10.1136\/gut.42.6.795","type":"journal-article","created":{"date-parts":[[2010,6,30]],"date-time":"2010-06-30T12:46:12Z","timestamp":1277901972000},"page":"795-798","source":"Crossref","is-referenced-by-count":70,"title":["Coexpression of gastrin and gastrin receptors (CCK-B and \u0394CCK-B) in gastrointestinal tumour cell lines"],"prefix":"10.1136","volume":"42","author":[{"given":"D F","family":"McWilliams","sequence":"first","affiliation":[{"name":"aAcademic Unit of Cancer Studies, Department of Surgery, \n\nbDivision of Clinical Laboratory Sciences, University Hospital, Nottingham, UK, \n\ncAphton Corporation, Woodland, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S A","family":"Watson","sequence":"additional","affiliation":[{"name":"aAcademic Unit of Cancer Studies, Department of Surgery, \n\nbDivision of Clinical Laboratory Sciences, University Hospital, Nottingham, UK, \n\ncAphton Corporation, Woodland, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D M","family":"Crosbee","sequence":"additional","affiliation":[{"name":"aAcademic Unit of Cancer Studies, Department of Surgery, \n\nbDivision of Clinical Laboratory Sciences, University Hospital, Nottingham, UK, \n\ncAphton Corporation, Woodland, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Michaeli","sequence":"additional","affiliation":[{"name":"aAcademic Unit of Cancer Studies, Department of Surgery, \n\nbDivision of Clinical Laboratory Sciences, University Hospital, Nottingham, UK, \n\ncAphton Corporation, Woodland, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Seth","sequence":"additional","affiliation":[{"name":"aAcademic Unit of Cancer Studies, Department of Surgery, \n\nbDivision of Clinical Laboratory Sciences, University Hospital, Nottingham, UK, \n\ncAphton Corporation, Woodland, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1998,6,1]]},"reference":[{"key":"2025090711255882000_42.6.795.1","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1517\/13543784.4.12.1253","article-title":"Gastrin antagonists and gastrointestinal tumours.","volume":"4","author":"Watson","year":"1995","journal-title":"Exp Opin Invest 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