{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T18:34:30Z","timestamp":1766428470337,"version":"3.46.0"},"reference-count":13,"publisher":"BMJ","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1970,2]]},"abstract":"<jats:p>\n                    An automated system is described for the microbiological assay of serum and red cell folate concentrations using a chloramphenicol-resistant strain of\n                    <jats:italic>L. casei<\/jats:italic>\n                    . Both serum and whole-blood haemolysates are assayed directly without previous deproteinization. Results by this method are comparable to those obtained by standard manual folate assays. Reproducibility within and between assays is excellent. Special advantages of the method are its simplicity of operation, economy of reagents, and capacity for dealing with large numbers of specimens (80-100 per hour). The latter makes it especially suitable for providing a regional diagnostic service and for population surveys of nutrition.\n                  <\/jats:p>","DOI":"10.1136\/jcp.23.1.54","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"54-59","source":"Crossref","is-referenced-by-count":38,"title":["Automation of the assay of folate in serum and whole blood"],"prefix":"10.1136","volume":"23","author":[{"given":"L.","family":"Millbank","sequence":"first","affiliation":[]},{"given":"R. E.","family":"Davis","sequence":"additional","affiliation":[]},{"given":"Mary","family":"Rawlins","sequence":"additional","affiliation":[]},{"given":"A. H.","family":"Waters","sequence":"additional","affiliation":[]}],"member":"239","published-online":{"date-parts":[[1970,2,1]]},"reference":[{"key":"ref_1","first-page":"275","article-title":"Statistical analysis showed a close correlation","volume":"5","author":"Baker, H.; Herbert, V.; Frank, O.; Pasher, I.; Hutner, S.H.","year":"1966","unstructured":"Statistical analysis showed a close correlation Baker, H., Herbert, V., Frank, O., Pasher, I., Hutner, S. H., between serum and whole blood folate concentrations measured by the automated and standard methods used in this laboratory (Waters and Mollin, 1961; Hoffbrand et al, 1966). There was also excellent reproducibility of results within and between assays. However, there was a dilution drift of approximately 20 % when serum and Wasserman, L. R., and Sobotka, H. (1959). A microbiologic method for datecting folic acid deficiency in man. Clin. Chem., 5, 275-280.","edition":"and","journal-title":"Clin. Chem; between serum and whole blood folate concentrations measured by the automated and standard methods used in this laboratory (Waters and Mollin, 1961; Hoffbrand et al"},{"key":"ref_2","first-page":"94","author":"Cowan, J.D.","year":"1969","unstructured":"Cowan, J. D. (1969). Ph.D. Thesis, University of London, pp. 94 and 166."},{"key":"ref_3","first-page":"81","article-title":"The assay and nature of folic acid activity in human serum","volume":"23","author":"Davis, R.E.; Nicol, D.J.; Kelly, A.","year":"1970","unstructured":"Davis, R. E., Nicol, D. J., and Kelly, A. (1970). J. clin. Path., 23 Herbert, V. (1961). The assay and nature of folic acid activity in human serum. J. clin. Invest., 40, 81-91.","journal-title":"J. clin. Path"},{"key":"ref_4","first-page":"307","article-title":"Experimental nutritional folate deficiency haemolysates were assayed at a dilution of 1\/200 instead of the usual 1\/100 dilution. This could also explain the slightly lower (approximately 5 %) serum and whole blood folate concentrations obtained by the automated method which was set up at a higher dilution than the manual method. A similar stimulatory effect of serum has been in man","volume":"75","author":"Herbert, V.","year":"1962","unstructured":"Herbert, V. (1962). Experimental nutritional folate deficiency haemolysates were assayed at a dilution of 1\/200 instead of the usual 1\/100 dilution. This could also explain the slightly lower (approximately 5 %) serum and whole blood folate concentrations obtained by the automated method which was set up at a higher dilution than the manual method. A similar stimulatory effect of serum has been in man. Trans. Ass. Amer. Phycns, 75, 307-320.","journal-title":"Trans. Ass. Amer. Phycns"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1136\/jcp.19.1.12","article-title":"Aseptic addition method for Lactobacillus casei assay of folate activity in human serum","volume":"19","author":"Herbert, V.","year":"1966","unstructured":"Herbert, V. (1966). Aseptic addition method for Lactobacillus casei assay of folate activity in human serum. J. clin. Path., 19, 12-16.","journal-title":"J. clin. Path"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1136\/jcp.19.1.17","article-title":"Method of assay of red cell folate activity and the value of the assay as a test for folate deficiency","volume":"19","author":"Hoffbrand, A.V.; Newcombe, B.F.A.; Mollin, D.L.","year":"1966","unstructured":"Hoffbrand, A. V., Newcombe, B. F. A., and Mollin, D. L. (1966). Method of assay of red cell folate activity and the value of the assay as a test for folate deficiency. J. clin. Path., 19, 17-28.","journal-title":"J. clin. Path"},{"key":"ref_7","first-page":"1","volume":"3","author":"Mollin, D.L.; Hoffbrand, A.V.","year":"1965","unstructured":"Mollin, D. L., and Hoffbrand, A. V. (1965). Ser. Haeinatol., 3, 1.","journal-title":"Ser. Haeinatol"},{"key":"ref_8","article-title":"Estimation of red cell folate activity","author":"Spray, G.H.","year":"1969","unstructured":"Spray, G. H. (1969). Estimation of red cell folate activity. J."},{"key":"ref_9","article-title":"using the whole serum aseptic addition assay described by Herbert","author":"reported by Cowan","year":"1969","unstructured":"reported by Cowan (1969) using the whole serum aseptic addition assay described by Herbert (1961, 1966)."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1016\/S0021-9258(18)65199-3","article-title":"Precursors of the folic acid-active factors of blood","volume":"221","author":"Toennies, G.; Usdin, E.; Phillips, P.M.","year":"1956","unstructured":"Toennies, G., Usdin, E., and Phillips, P. M. (1956). Precursors of the folic acid-active factors of blood. J. biol. Chem., 221, 855-863.","journal-title":"J. biol. Chem"},{"year":"1963","author":"Waters, A.H.","unstructured":"Waters, A. H. (1963). Ph.D. Thesis, University of London.","key":"ref_11"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1136\/jcp.14.4.335","article-title":"Studies on the folic acid activity of human serum","volume":"14","author":"Waters, A.H.; Mollin, D.L.","year":"1961","unstructured":"Waters, A. H., and Mollin, D. L. (1961). Studies on the folic acid activity of human serum. J. clin. Path., 14, 335-344.","journal-title":"J. clin. Path"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1111\/j.1365-2141.1963.tb06556.x","article-title":"Observations on the metabolism of folic acid in pernicious anaemia","volume":"9","author":"Waters, A.H.; Mollin, D.L.","year":"1963","unstructured":"Waters, A. H., and Mollin, D. L. (1963). Observations on the metabolism of folic acid in pernicious anaemia. Brit. J. Haemat., 9, 319-327.","journal-title":"Brit. J. Haemat"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.23.1.54","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T03:05:57Z","timestamp":1764990357000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.23.1.54"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1970,2]]},"references-count":13,"journal-issue":{"issue":"1","published-online":{"date-parts":[[1970,2,1]]},"published-print":{"date-parts":[[1970,2]]}},"alternative-id":["10.1136\/jcp.23.1.54"],"URL":"https:\/\/doi.org\/10.1136\/jcp.23.1.54","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1970,2]]}}}