{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T04:52:53Z","timestamp":1765601573557,"version":"3.48.0"},"reference-count":12,"publisher":"BMJ","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1972,10]]},"abstract":"<jats:p>Inhibition of streptolysin O activity by cholesterol depends on the latter being in free form. The normal esterified and protein-bound cholesterol fractions in serum do not influence streptolysin O activity. However, high cholesterol levels in rabbits fed cholesterol with cholic acid were associated with an increased antistreptolysin O effect. It is suggested that this occurs when all available protein sites are saturated and where a true `free' cholesterol fraction is present.<\/jats:p>\n                  <jats:p>Splitting the esterified cholesterol fraction of human sera with raised cholesterol levels, by means of pancreatin, produced an increased antistreptolysin O effect, again presumably because of saturation of protein-binding sites. Similarly, removal of non-esterified cholesterol from sera of cholesterolfed rabbits, by means of digitonin, reduced antistreptolysin O activity of the sera.<\/jats:p>\n                  <jats:p>Evidence is presented that combination of bovine serum albumin and streptolysin O has a steric hindrance effect on attachment of cholesterol to streptolysin O.<\/jats:p>\n                  <jats:p>The method described for the estimation of free cholesterol is extremely sensitive, being capable of detecting concentrations of less than 1\u00b70 \u03bcg\/ml.<\/jats:p>","DOI":"10.1136\/jcp.25.10.885","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"885-891","source":"Crossref","is-referenced-by-count":9,"title":["Some factors influencing the effect of cholesterol on streptolysin O activity"],"prefix":"10.1136","volume":"25","author":[{"given":"K. C.","family":"Watson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. P.","family":"Rose","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. J. C.","family":"Kerr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1972,10,1]]},"reference":[{"key":"ref_1","first-page":"192","article-title":"Some aspects of the mechanism of lysis of rabbit erythrocytes by streptolysin 0","volume-title":"Current Research on Group A Streptococcus; Excerpta Medica Foundation, Amsterdam.","author":"Alouf, J.E.; Raynaud, M.","year":"1968","unstructured":"Alouf, J. E., and Raynaud, M. (1968). Some aspects of the mechanism of lysis of rabbit erythrocytes by streptolysin 0. In Current Research on Group A Streptococcus, edited by R. Caravano, pp. 192-206. Excerpta Medica Foundation, Amsterdam."},{"key":"ref_2","first-page":"975","article-title":"Streptolysin 0 inhibition by serum gamma-G-globulin and beta-lipoprotein after blocking of non-esterified cholesterol by digitonin","volume":"75","author":"Badin, J.; Barillec, A.","year":"1970","unstructured":"Badin, J., and Barillec, A. (1970). Streptolysin 0 inhibition by serum gamma-G-globulin and beta-lipoprotein after blocking of non-esterified cholesterol by digitonin. J. Lab. clin. Med., 75, 975-982.","journal-title":"J. Lab. clin. Med"},{"key":"ref_3","first-page":"525","article-title":"The nonspecific inhibition of Streptolysin 0 in liver diseases. Its significance and clinical value","volume":"20","author":"Badin, J.; Cabau, N.; Levy, C.; Cachin, M.","year":"1962","unstructured":"Badin, J., Cabau, N., Levy, C., and Cachin, M. (1962). The nonspecific inhibition of Streptolysin 0 in liver diseases. Its significance and clinical value. (French). Ann. Biol. clin., 20, 525-541.","journal-title":"(French). Ann. Biol. clin"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1002\/path.1700490106","article-title":"The effect of cholesterol and of sera contaminated with bacteria on the haemolysins produced by haemolytic streptococci","volume":"49","author":"Hewitt, L.F.; Todd, E.W.","year":"1939","unstructured":"Hewitt, L. F., and Todd, E. W. (1939).The effect of cholesterol and of sera contaminated with bacteria on the haemolysins produced by haemolytic streptococci. J. Path. Bact., 49, 45-51.","journal-title":"J. Path. Bact"},{"key":"ref_5","first-page":"132","article-title":"Structure and Function in Biological Membranes","volume":"1","author":"Kavanau, J.L.","year":"1965","unstructured":"Kavanau, J. L. (1965). Structure and Function in Biological Membranes, vol. 1, p. 132. Holden-Day, San Francisco."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/0009-8981(64)90073-7","article-title":"Automated determination of serum total cholesterol","volume":"10","author":"Levine, J.B.; Zak, B.","year":"1964","unstructured":"Levine, J. B., and Zak, B. (1964). Automated determination of serum total cholesterol. Clin. chim. Acta, 10, 381-384.","journal-title":"Clin. chim. Acta"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1159\/000229688","article-title":"Investigations concerning the action of streptolysin 0 and its unspezific inhibition by lipids","volume":"29","year":"1966","unstructured":"Petersen, K. F., Nowak, P., Thiele, 0. W., and Urbaschek, B. (1966). Investigations concerning the action of streptolysin 0 and its unspezific inhibition by lipids. Int. Arch. Allergy, 29, 69-81.","journal-title":"Int. Arch. Allergy"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"22","DOI":"10.3181\/00379727-59-14964","article-title":"A modification of the technic for determination of the antistreptolysin titer","volume":"59","author":"Rantz, L.A.; Randall, E.","year":"1945","unstructured":"Rantz, L. A., and Randall, E. (1945). A modification of the technic for determination of the antistreptolysin titer. Proc. Soc. exp. Biol. (N. Y.), 59, 22-25.","journal-title":"Proc. Soc. exp. Biol. (N. Y.)"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1172\/JCI102998","article-title":"The use of haemolysin inhibition in the study of experimental and clinical hyperlipemia; the nonspecific inhibition of streptolysin 0 by serum lipoproteins","volume":"33","author":"Stollerman, G.H.","year":"1954","unstructured":"Stollerman, G. H. (1954). The use of haemolysin inhibition in the study of experimental and clinical hyperlipemia; the nonspecific inhibition of streptolysin 0 by serum lipoproteins. J. clin. Invest., 33, 1233-1241.","journal-title":"J. clin. Invest"},{"key":"ref_10","article-title":"Unpublished observations","author":"C., Watson K.; Rose, T.P.; Kerr, E.J.C.","year":"1971","unstructured":"Watson. K. C., Rose, T. P., and Kerr, E. J. C. (1971). Unpublished observations."},{"key":"ref_11","unstructured":"The September 1972 Issue","article-title":"The September 1972 Issue"},{"key":"ref_12","unstructured":"THE SEPTEMBER 1972 ISSUE CONTAINS THE FOLLOWING PAPERS","article-title":"THE SEPTEMBER 1972 ISSUE CONTAINS THE FOLLOWING PAPERS"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.25.10.885","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T04:49:36Z","timestamp":1765601376000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.25.10.885"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,10]]},"references-count":12,"journal-issue":{"issue":"10","published-online":{"date-parts":[[1972,10,1]]},"published-print":{"date-parts":[[1972,10]]}},"alternative-id":["10.1136\/jcp.25.10.885"],"URL":"https:\/\/doi.org\/10.1136\/jcp.25.10.885","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1972,10]]}}}