{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T07:13:37Z","timestamp":1765610017152,"version":"3.44.0"},"reference-count":27,"publisher":"BMJ","issue":"4","license":[{"start":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T00:00:00Z","timestamp":1589846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/bmj.com\/coronavirus\/usage"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2020,5,1]]},"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:sec>\n                  <jats:title>Aims<\/jats:title>\n                  <jats:p>In the time of COVID-19, predictive molecular pathology laboratories must still timely select oncological patients for targeted treatments. However, the need to respect social distancing measures may delay results generated by laboratory-developed tests based on sequential steps a long hands-on time. Laboratory workflows should now be simplified.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The organisation of the University of Naples Federico II predictive pathology laboratory was assessed before (March\u2013April 2019) and during (March\u2013April 2020) the Italian lockdown.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The number of patients undergoing single or multiple biomarker testing was similar in 2019 (n=43) and in 2020 (n=45). Considering adequate samples for molecular testing, before the outbreak, next-generation sequencing was mostly used (35\/42, 83.3%). Testing six genes had a reagent cost of \u20ac98\/patient. Conversely, in 2020, almost all cases (38\/41, 92.7%) were analysed by automated testing. This latter had for any single assay\/gene a significant reagent cost (\u20ac95\u2013\u20ac136) and a faster mean turnaround time (5.3 vs 7.9 working days).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>In the times of coronavirus, laboratory fully automated platforms simplify predictive molecular testing. Laboratory staff may be more safely and cost-effectively managed.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/jclinpath-2020-206711","type":"journal-article","created":{"date-parts":[[2020,5,20]],"date-time":"2020-05-20T05:44:36Z","timestamp":1589953476000},"page":"234-237","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":11,"title":["Predictive molecular pathology in the time of COVID-19"],"prefix":"10.1136","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3211-9957","authenticated-orcid":false,"given":"Umberto","family":"Malapelle","sequence":"first","affiliation":[{"name":"Public Health, University of Naples Federico II, Naples, Italy"}]},{"given":"Caterina","family":"De Luca","sequence":"additional","affiliation":[{"name":"Public Health, University of Naples Federico II, Naples, Italy"}]},{"given":"Antonino","family":"Iaccarino","sequence":"additional","affiliation":[{"name":"Public Health, University of Naples Federico II, Naples, Italy"}]},{"given":"Francesco","family":"Pepe","sequence":"additional","affiliation":[{"name":"Public Health, University of Naples Federico II, Naples, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6429-0620","authenticated-orcid":false,"given":"Pasquale","family":"Pisapia","sequence":"additional","affiliation":[{"name":"Public Health, University of Naples Federico II, Naples, Italy"}]},{"given":"Maria","family":"Russo","sequence":"additional","affiliation":[{"name":"Public Health, University of Naples Federico II, Naples, Italy"}]},{"given":"Roberta","family":"Sgariglia","sequence":"additional","affiliation":[{"name":"Public Health, University of Naples Federico II, Naples, Italy"}]},{"given":"Mariantonia","family":"Nacchio","sequence":"additional","affiliation":[{"name":"Public Health, University of Naples Federico II, Naples, Italy"}]},{"given":"Elena","family":"Vigliar","sequence":"additional","affiliation":[{"name":"Public Health, University of Naples Federico II, Naples, Italy"}]},{"given":"Claudio","family":"Bellevicine","sequence":"additional","affiliation":[{"name":"Public Health, University of Naples Federico II, Naples, Italy"}]},{"given":"Fernando C","family":"Schmitt","sequence":"additional","affiliation":[{"name":"Pathology, IPATIMUP and Medical Faculty of Porto, Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1630-5805","authenticated-orcid":false,"given":"Giancarlo","family":"Troncone","sequence":"additional","affiliation":[{"name":"Public Health, University of Naples Federico II, Naples, Italy"}]}],"member":"239","published-online":{"date-parts":[[2020,5,19]]},"reference":[{"key":"2025092509144344000_74.4.234.1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30185-9"},{"key":"2025092509144344000_74.4.234.2","unstructured":"World Health Organization (WHO) . 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