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The stimulation by phosphate is relieved by the addition of magnesium ion, to the assay system.The effects on enzymatic activity were studied when the supernatant and mitochondrial malate dehydrogenase were subjected to freezing and thawing in various media. Freezing and thawing of the mitochondrial enzyme in Tris or citrate buffers with or without mercaptoethanol results in small activity loss. Supernatant malate dehydrogenase displays a marked loss in activity when frozen quickly in the presence of phosphate and mercaptoethanol; if mercaptoethanol is omitted, activity loss is small. NADH, oxalacetic acid (OAA), NAD, and malate protect against activity loss in the phosphate\u2013mercaptoethanol system. Repeated freeze\u2013thawing of mitochondrial malate dehydrogenase in the presence of both phosphate and magnesium results in extensive losses in activity. In all cases slow freezing produces little activity loss for either enzyme.<\/jats:p>","DOI":"10.1139\/o67-075","type":"journal-article","created":{"date-parts":[[2009,12,17]],"date-time":"2009-12-17T22:08:22Z","timestamp":1261087702000},"page":"641-650","source":"Crossref","is-referenced-by-count":24,"title":["THE EFFECTS OF IONS AND FREEZE\u2013THAWING ON SUPERNATANT AND MITOCHONDRIAL MALATE DEHYDROGENASE"],"prefix":"10.1139","volume":"45","author":[{"given":"D. J.","family":"Blonde","sequence":"first","affiliation":[]},{"given":"E. J.","family":"Kresack","sequence":"additional","affiliation":[]},{"given":"G. W.","family":"Kosicki","sequence":"additional","affiliation":[]}],"member":"155","reference":[{"key":"p_1\/p_1_1","first-page":"273","volume":"331","author":"ZEBE T.","year":"1959","journal-title":"BUGHER. Biockem. Z."},{"key":"p_2\/p_2_1","first-page":"297","volume":"331","author":"DELBRWCK H.","year":"1959","journal-title":"BUCHER. Biochem. 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