{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T06:03:40Z","timestamp":1779343420545,"version":"3.51.4"},"reference-count":39,"publisher":"Canadian Science Publishing","issue":"11-12","license":[{"start":{"date-parts":[[1995,12,1]],"date-time":"1995-12-01T00:00:00Z","timestamp":817776000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochem. Cell Biol."],"published-print":{"date-parts":[[1995,12,1]]},"abstract":"<jats:p> Pseudouridine (\u03a8), the most common single modified nucleoside in ribosomal RNA, has been positioned in the small subunit (SSU) and large subunit (LSU) RNAs of a number of representative species. Most of the information has been obtained by application of a rapid primed reverse transcriptase sequencing technique. The locations of these \u03a8 residues have been compared. Many sites for \u03a8 are the same among species, but others are distinct. In general, the percentage \u03a8 in multicellular eukaryotes is greater than in prokaryotes. In LSU RNA, the \u03a8 residues are strongly clustered in three domains, all of which are near or connected to the peptidyl transferase center. There is no apparent clustering of \u03a8 in SSU RNA. The \u03a8 sites in LSU RNA overlap those for the methylated nucleosides, but this is not the case in SSU RNA. There are 265 \u03a8 sites known to nucleotide resolution, of which 246 are in defined secondary structures, and 112 of these are in nonidentical structural contexts. All 246 \u03a8 sites can be classified into five structural types. Two Escherichia coli \u03a8 synthases have been cloned and characterized, one for \u03a8516 in SSU RNA and one for \u03a8746 in LSU RNA. The \u03a8746 synthase recognizes free RNA, but the \u03a8516 enzyme requires an intermediate RNP particle. Possible functional roles for \u03a8 in the ribosome are discussed.Key words: pseudouridine, sequencing, modified nucleosides, ribosomal RNA. <\/jats:p>","DOI":"10.1139\/o95-099","type":"journal-article","created":{"date-parts":[[2009,12,23]],"date-time":"2009-12-23T19:51:08Z","timestamp":1261597868000},"page":"915-924","source":"Crossref","is-referenced-by-count":63,"title":["The pseudouridine residues of ribosomal RNA"],"prefix":"10.1139","volume":"73","author":[{"given":"James","family":"Ofengand","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrei","family":"Bakin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Wrzesinski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelvin","family":"Nurse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Byron G.","family":"Lane","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"p_1\/p_1_1","doi-asserted-by":"crossref","first-page":"9754","DOI":"10.1021\/bi00088a030","volume":"32","author":"Bakin A.","year":"1993","journal-title":"Biochemistry"},{"key":"p_2\/p_2_1","doi-asserted-by":"crossref","first-page":"3290","DOI":"10.1093\/nar\/23.16.3290","volume":"23","author":"Bakin A","year":"1995","journal-title":"Nucleic Acids Res."},{"key":"p_3\/p_3_1","unstructured":"Bakin, A., and Ofengand,J. 1995b.Mapping pseudouridineresidues in RNA to nucleotide resolution. 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