{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T10:53:39Z","timestamp":1769597619503,"version":"3.49.0"},"reference-count":46,"publisher":"Canadian Science Publishing","issue":"3","license":[{"start":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:00:00Z","timestamp":1172707200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:p>Confocal laser scanning microscopy and fluorescent lectin-binding analyses (FLBA) were used to study the form, arrangement, and composition of exopolymeric substances (EPS) surrounding naturally occurring microcolonies in biofilms. FLBA, using multiple lectin staining and multichannel imaging, indicated that the EPS of many microcolonies exhibit distinct multiple binding regions. A common pattern in the microcolonies is a three zone arrangement with cell-associated, intercellular, and an outer layer of EPS covering the exterior of the colony. Differential binding of lectins suggests that there are differences in the glycoconjugate composition or their arrangement in the EPS of microcolonies. The combination of FLBA with fluorescent in situ hybridization (FISH) indicates that the colonies consist of the major groups, \u03b1- and \u03b2-Proteobacteria. It is suggested that the EPS arrangement observed provides a physical structuring mechanism that can segregate extracellular activities at the microscale.<\/jats:p>","DOI":"10.1139\/w06-146","type":"journal-article","created":{"date-parts":[[2007,5,30]],"date-time":"2007-05-30T19:46:13Z","timestamp":1180554373000},"page":"450-458","source":"Crossref","is-referenced-by-count":95,"title":["In situ evidence for microdomains in the polymer matrix of bacterial microcolonies"],"prefix":"10.1139","volume":"53","author":[{"given":"J.R.","family":"Lawrence","sequence":"first","affiliation":[{"name":"National Water Research Institute, 11 Innovation Boulevard, Saskatoon, SK S7N\u00a03H5, Canada."},{"name":"Department of River Ecology, Helmholtz Centre for Environmental Research \u2013 UFZ, Brueckstrasse 3A, 39114 Magdeburg, Germany."}]},{"given":"G.D.W.","family":"Swerhone","sequence":"additional","affiliation":[{"name":"National Water Research Institute, 11 Innovation Boulevard, Saskatoon, SK S7N\u00a03H5, Canada."},{"name":"Department of River Ecology, Helmholtz Centre for Environmental Research \u2013 UFZ, Brueckstrasse 3A, 39114 Magdeburg, Germany."}]},{"given":"U.","family":"Kuhlicke","sequence":"additional","affiliation":[{"name":"National Water Research Institute, 11 Innovation Boulevard, Saskatoon, SK S7N\u00a03H5, Canada."},{"name":"Department of River Ecology, Helmholtz Centre for Environmental Research \u2013 UFZ, Brueckstrasse 3A, 39114 Magdeburg, Germany."}]},{"given":"T.R.","family":"Neu","sequence":"additional","affiliation":[{"name":"National Water Research Institute, 11 Innovation Boulevard, Saskatoon, SK S7N\u00a03H5, Canada."},{"name":"Department of River Ecology, Helmholtz Centre for Environmental Research \u2013 UFZ, Brueckstrasse 3A, 39114 Magdeburg, Germany."}]}],"member":"155","reference":[{"key":"atypb1\/ref1","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1111\/j.1365-2672.1992.tb05009.x","volume":"73","author":"Allison D.G.","year":"1992","journal-title":"J. Appl. Bacteriol."},{"key":"atypb2\/ref2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-7012(84)90034-4"},{"key":"atypb3\/ref3","first-page":"1","volume":"18","author":"Alsop R.M.","year":"1983","journal-title":"Prog. Ind. Microbiol."},{"key":"atypb4\/ref4","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1128\/JB.172.2.762-770.1990","volume":"172","author":"Amann R.I","year":"1990","journal-title":"J. Bacteriol."},{"key":"atypb5\/ref5","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.9.5443-5452.2003"},{"key":"atypb6\/ref6","doi-asserted-by":"crossref","first-page":"1491","DOI":"10.1099\/13500872-145-6-1491","volume":"145","author":"Beech I.","year":"1999","journal-title":"Microbiology (Reading, U.K.)"},{"key":"atypb7\/ref7","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1128\/MMBR.55.4.684-705.1991","volume":"55","author":"Beveridge T.J.","year":"1991","journal-title":"Microbiol. Rev."},{"key":"atypb8\/ref8","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(01)00354-2"},{"key":"atypb9\/ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2004.11.006"},{"key":"atypb10\/ref10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-4684-7609-5_1","volume":"12","author":"Caldwell D.E.","year":"1992","journal-title":"Adv. Microb. Ecol."},{"key":"atypb11\/ref11","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S0141-3910(97)00179-1","volume":"59","author":"Ertesvag H.","year":"1998","journal-title":"Polym. Degrad. Stabil."},{"key":"atypb12\/ref12","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(87)81344-3"},{"key":"atypb13\/ref13","unstructured":"Hirsch, P. 1984. Microcolony formation and consortia.InMicrobial adhesion and aggregation.Edited byK.C.\u00a0Marshall. Dahlem Konferenzen, Springer-Verlag, Berlin, pp. 373\u2013393."},{"key":"atypb14\/ref14","unstructured":"Hoffman, M., and Decho, A.W. 1999. Extracellular enzymes within microbial biofilms and the role of the extracellular polymeric matrix.InMicrobial extracellular polymeric substances.Edited byJ. Wingender, T.R.\u00a0Neu, and H.C. Flemming. Springer-Verlag, Berlin, pp. 217\u2013227."},{"key":"atypb15\/ref15","doi-asserted-by":"crossref","first-page":"3487","DOI":"10.1128\/AEM.66.8.3487-3491.2000","volume":"66","author":"Johnsen A.R.","year":"2000","journal-title":"App. Environ. Microbiol."},{"key":"atypb16\/ref16","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/BF02011567","volume":"13","author":"Lawrence J.R.","year":"1987","journal-title":"Microb. Ecol."},{"key":"atypb17\/ref17","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/S0076-6879(99)10011-9","volume":"310","author":"Lawrence J.R.","year":"1999","journal-title":"Methods Enzymol."},{"key":"atypb18\/ref18","doi-asserted-by":"publisher","DOI":"10.1007\/BF02011566"},{"key":"atypb19\/ref19","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1128\/AEM.60.4.1166-1173.1994","volume":"60","author":"Lawrence J.R.","year":"1994","journal-title":"Appl. Environ. Microbiol."},{"key":"atypb20\/ref20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-4684-7724-5_1","volume":"14","author":"Lawrence J.R.","year":"1995","journal-title":"Adv. Microb. Ecol."},{"key":"atypb21\/ref21","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(00)00195-0"},{"key":"atypb22\/ref22","unstructured":"Lawrence, J.R., Korber, D.R., Wolfaardt, G.M., Caldwell, D.E., and Neu, T.R. 2002. Analytical imaging and microscopy techniques.InManual of environmental microbiology. 2nd ed.Edited byC.J. Hurst, Ronald L. Crawford, G.R. Knudsen, M. McInerney, and L.D. Stetzenbach. American Society for Microbiology Press, Washington, D.C. pp. 39\u201361."},{"key":"atypb23\/ref23","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.7.4326-4339.2004"},{"key":"atypb24\/ref24","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/0048-9697(95)04546-D","volume":"165","author":"Leppard G.G.","year":"1995","journal-title":"Sci. Total Environ."},{"key":"atypb25\/ref25","doi-asserted-by":"crossref","first-page":"205","DOI":"10.3354\/ame014205","volume":"14","author":"Lienemann C.-P.","year":"1998","journal-title":"Aquat. Microb. Ecol."},{"key":"atypb26\/ref26","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1128\/jb.179.1.31-40.1997","volume":"179","author":"L\u00fcnsdorf H.","year":"1997","journal-title":"J. Bacteriol."},{"key":"atypb27\/ref27","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/S0723-2020(11)80121-9","volume":"15","author":"Manz W.","year":"1992","journal-title":"Syst. Appl. Microbiol."},{"key":"atypb28\/ref28","doi-asserted-by":"crossref","first-page":"2293","DOI":"10.1128\/AEM.59.7.2293-2298.1993","volume":"59","author":"Manz W.","year":"1993","journal-title":"Appl. Environ. Microbiol."},{"key":"atypb29\/ref29","doi-asserted-by":"publisher","DOI":"10.1007\/s002489900148"},{"key":"atypb30\/ref30","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/S0723-2020(99)80065-4","volume":"22","author":"Meier H.","year":"1999","journal-title":"Syst. Appl. Microbiol."},{"key":"atypb31\/ref31","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0580-9517(04)34004-3","volume":"34","author":"Neu T.R.","year":"2005","journal-title":"Methods Microbiol."},{"key":"atypb32\/ref32","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1099\/00221287-147-2-299","volume":"147","author":"Neu T.","year":"2001","journal-title":"Microbiology (Reading, U.K.)"},{"key":"atypb33\/ref33","doi-asserted-by":"crossref","first-page":"283","DOI":"10.3354\/ame038283","volume":"38","author":"Neu T.R.","year":"2005","journal-title":"Aquat. Microb. Ecol."},{"key":"atypb34\/ref34","doi-asserted-by":"publisher","DOI":"10.1016\/0144-8617(84)90051-1"},{"key":"atypb35\/ref35","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6215(97)00026-8"},{"key":"atypb36\/ref36","doi-asserted-by":"crossref","first-page":"3605","DOI":"10.1128\/AEM.62.10.3605-3613.1996","volume":"62","author":"Rabus R.","year":"1996","journal-title":"Appl. Environ. Microbiol."},{"key":"atypb37\/ref37","doi-asserted-by":"publisher","DOI":"10.1007\/s002530051051"},{"key":"atypb38\/ref38","doi-asserted-by":"crossref","first-page":"2849","DOI":"10.1099\/00221287-140-10-2849","volume":"140","author":"Roller C.","year":"1994","journal-title":"Microbiology (Reading, U.K.)"},{"key":"atypb39\/ref39","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.52.1.81"},{"key":"atypb40\/ref40","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.4.2445-2451.2004"},{"key":"atypb41\/ref41","unstructured":"Stahl, D.A., and Amann, R. 1991. Development and application of nucleic acid probes in bacterial systematics.InSequencing and hybridization techniques in bacterial systematics.Edited byE.\u00a0Stackebrandt and M. Goodfellow. John Wiley, Chichester, UK. pp.\u00a0205\u2013248."},{"key":"atypb42\/ref42","doi-asserted-by":"crossref","unstructured":"Sutherland, I.W. 1990. Biotechnology of microbial exopolysaccharides. Cambridge University Press. Cambridge, UK.","DOI":"10.1017\/CBO9780511525384"},{"key":"atypb43\/ref43","unstructured":"Sutherland, I.W. 1999. Biofilm Exopolysaccharides.InMicrobial extracellular substances.Edited byJ. Wingender, T.R. Neu, and H.C. Flemming. Springer-Verlag, Berlin. pp. 73\u201389."},{"key":"atypb44\/ref44","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(01)02012-1"},{"key":"atypb45\/ref45","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.1.428-435.2005"},{"key":"atypb46\/ref46","unstructured":"Wingender, J., Jaeger, K.E., and Flemming, H.C. 1999. Interaction between extracellular polysaccharides and enzymes.InMicrobial extracellular polymeric substances.Edited byJ.\u00a0Wingender, T.R. Neu, and H.C. Flemming. Springer, Germany. pp. 231\u2013251."}],"container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/full-xml\/10.1139\/W06-146","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/W06-146","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,4,22]],"date-time":"2020-04-22T14:04:06Z","timestamp":1587564246000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/W06-146"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,3]]},"references-count":46,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2007,3]]}},"alternative-id":["10.1139\/W06-146"],"URL":"https:\/\/doi.org\/10.1139\/w06-146","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,3]]}}}