{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:23:26Z","timestamp":1788481406145,"version":"build-2803163510"},"reference-count":41,"publisher":"Canadian Science Publishing","issue":"3","license":[{"start":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T00:00:00Z","timestamp":1456790400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Appl. Physiol. Nutr. Metab."],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:p> Mitochondrial quality control and apoptosis have been described as key components in the pathogenesis of nonalcoholic steatohepatitis (NASH); exercise is recognized as a nonpharmacological strategy to counteract NASH-associated consequences. We aimed to analyze the effect of voluntary physical activity (VPA) and endurance training (ET) against NASH-induced mitochondrial permeability transition pore (mPTP) opening and mitochondrial and cellular quality control deleterious alterations. Forty-eight male Sprague\u2013Dawley rats were divided into standard-diet sedentary (SS, n = 16), standard-diet VPA (n = 8), high-fat diet sedentary (HS, n = 16), and high-fat diet VPA (n = 8). After 9 weeks of diet treatment, half of the SS and HS groups were engaged in an ET program for 8 weeks, 5 days\/week, 1 h\/day. Liver mPTP susceptibility through osmotic swelling, mPTP-related proteins (cyclophilin D, Sirtuin3, Cofilin-1), markers of mitochondrial biogenesis ((mitochondrial transcription factor A (Tfam) and peroxisome proliferator-activated receptor gamma co-activator protein (PGC-1\u03b1)), dynamics (Mitofusin 1 (Mfn1), Mitofusin 2 (Mfn2), Dynamin related protein 1, and Optic atrophy 1)), auto\/mitophagy (Beclin-1, microtubule-associated protein 1 light chain 3, p62, PINK1, and Parkin), and apoptotic signaling (Bax, Bcl-2) and caspases-like activities were assessed. HS animals showed an increased susceptibility to mPTP, compromised expression of Tfam, Mfn1, PINK1, and Parkin and an increase in Bax content (HS vs. SS). ET and VPA improved biogenesis-related proteins (PGC-1\u03b1) and autophagy signaling (Beclin-1 and Beclin-1\/Bcl-2 ratio) and decreased apoptotic signaling (caspases 8 activity, Bax content, and Bax\/Bcl-2 ratio). However, only ET decreased mPTP susceptibility and positively modulated Bcl-2, Tfam, Mfn1, Mfn2, PINK1, and Parkin content. In conclusion, exercise reduces the increased susceptibility to mPTP induced by NASH and promotes the increase of auto\/mitophagy and mitochondrial fusion towards a protective phenotype. <\/jats:p>","DOI":"10.1139\/apnm-2015-0470","type":"journal-article","created":{"date-parts":[[2015,11,17]],"date-time":"2015-11-17T22:40:34Z","timestamp":1447800034000},"page":"298-306","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":77,"title":["Exercise mitigates mitochondrial permeability transition pore and quality control mechanisms alterations in nonalcoholic steatohepatitis"],"prefix":"10.1139","volume":"41","author":[{"given":"In\u00eas O.","family":"Gon\u00e7alves","sequence":"first","affiliation":[{"name":"Research Center in Physical Activity, Health and Leisure, Faculty of Sport, University of Porto, Rua Dr. Pl\u00e1cido Costa, n\u00b0 91 4200-450 Porto, Portugal."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuel","family":"Passos","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine, University of Porto, Al. Prof. Hern\u00e2ni Monteiro, 4200-319 Porto, Portugal."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e1tia V.","family":"Diogo","sequence":"additional","affiliation":[{"name":"CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Rua Larga Faculty of Medicine, P\u00f3lo I, 3004-504 Coimbra, Portugal."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00edlvia","family":"Rocha-Rodrigues","sequence":"additional","affiliation":[{"name":"Research Center in Physical Activity, Health and Leisure, Faculty of Sport, University of Porto, Rua Dr. Pl\u00e1cido Costa, n\u00b0 91 4200-450 Porto, Portugal."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estela","family":"Santos-Alves","sequence":"additional","affiliation":[{"name":"Research Center in Physical Activity, Health and Leisure, Faculty of Sport, University of Porto, Rua Dr. Pl\u00e1cido Costa, n\u00b0 91 4200-450 Porto, Portugal."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo J.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Rua Larga Faculty of Medicine, P\u00f3lo I, 3004-504 Coimbra, Portugal."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ant\u00f3nio","family":"Ascens\u00e3o","sequence":"additional","affiliation":[{"name":"Research Center in Physical Activity, Health and Leisure, Faculty of Sport, University of Porto, Rua Dr. Pl\u00e1cido Costa, n\u00b0 91 4200-450 Porto, Portugal."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Magalh\u00e3es","sequence":"additional","affiliation":[{"name":"Research Center in Physical Activity, Health and Leisure, Faculty of Sport, University of Porto, Rua Dr. Pl\u00e1cido Costa, n\u00b0 91 4200-450 Porto, Portugal."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1586\/egh.11.4"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1042\/CS20100254"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.mito.2013.07.002"},{"issue":"4","key":"refg4\/ref4","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1152\/physrev.1999.79.4.1127","volume":"79","author":"Bernardi P.","year":"1999","journal-title":"Physiol. Rev."},{"key":"refg5\/ref5","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2013.00095"},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M503062200"},{"key":"refg7\/ref7","doi-asserted-by":"publisher","DOI":"10.1054\/ceca.2000.0177"},{"key":"refg8\/ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2006.01.016"},{"key":"refg9\/ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2011.02.003"},{"key":"refg10\/ref10","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201312066"},{"key":"refg11\/ref11","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00127.2012"},{"key":"refg12\/ref12","doi-asserted-by":"publisher","DOI":"10.2741\/1765"},{"key":"refg13\/ref13","doi-asserted-by":"publisher","DOI":"10.1111\/eci.12146"},{"key":"refg14\/ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.mito.2014.03.012"},{"key":"refg15\/ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2014.03.010"},{"key":"refg16\/ref16","doi-asserted-by":"publisher","DOI":"10.1042\/bj3540243"},{"issue":"2","key":"refg17\/ref17","first-page":"72","volume":"17","author":"Grattagliano I.","year":"2013","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"issue":"12","key":"refg18\/ref18","doi-asserted-by":"crossref","first-page":"914","DOI":"10.18632\/aging.100252","volume":"2","author":"Hafner A.V.","year":"2010","journal-title":"Aging (Albany NY)"},{"issue":"7","key":"refg19\/ref19","first-page":"1062","volume":"33","author":"Kang M.","year":"2013","journal-title":"Nan Fang Yi Ke Da Xue Xue Bao"},{"key":"refg20\/ref20","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01231.2007"},{"key":"refg21\/ref21","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2010.488"},{"key":"refg22\/ref22","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1968"},{"key":"refg23\/ref23","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00013.2006"},{"key":"refg24\/ref24","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.242412"},{"key":"refg25\/ref25","doi-asserted-by":"publisher","DOI":"10.1089\/rej.2005.8.3"},{"key":"refg26\/ref26","doi-asserted-by":"publisher","DOI":"10.1016\/j.mito.2010.07.005"},{"key":"refg27\/ref27","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.8.1863"},{"key":"refg28\/ref28","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2010.07.006"},{"key":"refg29\/ref29","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00510.2010"},{"key":"refg30\/ref30","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(98)00108-X"},{"key":"refg31\/ref31","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2011.180"},{"key":"refg32\/ref32","doi-asserted-by":"publisher","DOI":"10.1111\/j.1872-034X.2011.00824.x"},{"key":"refg33\/ref33","doi-asserted-by":"publisher","DOI":"10.1111\/eci.12135"},{"key":"refg34\/ref34","doi-asserted-by":"publisher","DOI":"10.2174\/092986710791698530"},{"key":"refg35\/ref35","doi-asserted-by":"publisher","DOI":"10.1586\/egh.11.11"},{"key":"refg36\/ref36","doi-asserted-by":"publisher","DOI":"10.1016\/j.mito.2008.07.008"},{"key":"refg37\/ref37","doi-asserted-by":"publisher","DOI":"10.1016\/j.mito.2013.09.002"},{"key":"refg38\/ref38","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2009.169060"},{"key":"refg39\/ref39","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2010.3779"},{"key":"refg40\/ref40","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2010.01.016"},{"issue":"10","key":"refg41\/ref41","doi-asserted-by":"crossref","first-page":"1866","DOI":"10.1093\/jn\/138.10.1866","volume":"138","author":"Wang Y.","year":"2008","journal-title":"J. Nutr."}],"container-title":["Applied Physiology, Nutrition, and Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdnsciencepub.com\/doi\/full-xml\/10.1139\/apnm-2015-0470","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdnsciencepub.com\/doi\/pdf\/10.1139\/apnm-2015-0470","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T15:17:36Z","timestamp":1751469456000},"score":1,"resource":{"primary":{"URL":"https:\/\/cdnsciencepub.com\/doi\/10.1139\/apnm-2015-0470"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3]]},"references-count":41,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2016,3]]}},"alternative-id":["10.1139\/apnm-2015-0470"],"URL":"https:\/\/doi.org\/10.1139\/apnm-2015-0470","relation":{},"ISSN":["1715-5312","1715-5320"],"issn-type":[{"value":"1715-5312","type":"print"},{"value":"1715-5320","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,3]]},"assertion":[{"value":"2015-09-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-11-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-11-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}