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This study analyzes the influence of diclofenac on the toll-like receptor-nuclear factor kappa B (TLR-NF-\u03baB) pathway in skeletal muscle of rats submitted to acute eccentric exercise. Twenty male Wistar rats were divided into 4 groups: control-saline, control-diclofenac, exercise-saline, and exercise-diclofenac. Diclofenac or saline were administered for 7 days prior to an acute eccentric exercise bout. The inflammatory status was evaluated through mRNA levels of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), interleukin-6 (IL-6), IL-1\u03b2, and tumor necrosis factor alpha (TNF-\u03b1), and protein content of COX-2, IL-6, and TNF-\u03b1 in vastus lateralis muscle. Data obtained showed that a single bout of eccentric exercise significantly increased COX-2 gene expression. Similarly, mRNA expression and protein content of other inflammation-related genes also increased after the acute exercise. However, these effects were attenuated in the exercise + diclofenac group. TLR4, myeloid differentiation primary response gene 88 (MyD88), and p65 were also upregulated after the acute eccentric bout and the effect was blunted by the anti-inflammatory drug. These findings suggest that pretreatment with diclofenac may represent an effective tool to ameliorate the pro-inflammatory status induced by acute exercise in rat skeletal muscle possibly through an attenuation of the TLR4-NF-\u03baB signaling pathway. <\/jats:p>","DOI":"10.1139\/apnm-2016-0593","type":"journal-article","created":{"date-parts":[[2017,2,24]],"date-time":"2017-02-24T17:33:21Z","timestamp":1487957601000},"page":"757-764","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":25,"title":["Diclofenac pretreatment modulates exercise-induced inflammation in skeletal muscle of rats through the TLR4\/NF-\u03baB pathway"],"prefix":"10.1139","volume":"42","author":[{"given":"R\u00f4mulo Pillon","family":"Barcelos","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Bioexperimenta\u00e7\u00e3o, Universidade de Passo Fundo, RS, 99052-900, Brazil."},{"name":"Institute of Biomedicine, University of Le\u00f3n, Campus Universitario, 24071 Le\u00f3n, Spain."},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Bioqu\u00edmica Toxicol\u00f3gica, Centro de Ci\u00eancias Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900 Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guilherme","family":"Bresciani","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Rendimiento F\u00edsico y Salud Escuela de Educaci\u00f3n F\u00edsica, Pontificia Universidad Cat\u00f3lica de Valparaiso, Valparaiso, 2530388 Chile."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Jos\u00e9","family":"Cuevas","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine, University of Le\u00f3n, Campus Universitario, 24071 Le\u00f3n, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"Mart\u00ednez-Fl\u00f3rez","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine, University of Le\u00f3n, Campus Universitario, 24071 Le\u00f3n, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e9lix Alexandre Antunes","family":"Soares","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Bioqu\u00edmica Toxicol\u00f3gica, Centro de Ci\u00eancias Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Gonz\u00e1lez-Gallego","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine, University of Le\u00f3n, Campus Universitario, 24071 Le\u00f3n, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","first-page":"1","volume":"311","author":"Akira S.","year":"2006","journal-title":"Curr. 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