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Diosmin (Dios), a citrus bioflavonoid, is known to possess potent antioxidant, anti-inflammatory, and anti-apoptotic properties. Purpose: To explore the molecular mechanisms that underlie radiation-induced liver fibrosis, and to evaluate the possible influence of Dios on the miR-17-5p\u2013Wnt\u2013\u03b2-catenin signaling axis during fibrogenesis provoked by irradiation (IRR) in rats. Also, the effect of Dios on hepatic peroxisome proliferator activated receptor-\u03b3 (PPAR-\u03b3) expression as a regulator for HSC activation was considered. Methods: We administered 100 mg\u00b7(kg body mass)<jats:sup>\u20131<\/jats:sup>\u00b7day<jats:sup>\u20131<\/jats:sup> (per oral) of Dios were administered to IRR-exposed rats (overall dose of 12 Gy on 6 fractions of 2 Gy each) for 6 successive weeks. Results: Data analysis revealed that Dios treatment mitigated oxidative stress, enhanced antioxidant defenses, alleviated hepatic inflammatory responses, abrogated pro-fibrogenic cytokines, and stimulated PPAR-\u03b3 expression. Dios treatment repressed the miR-17-5p activated Wnt\u2013\u03b2-catenin signaling induced by IRR. Moreover, Dios treatment restored the normal hepatic architecture and reversed pathological alterations induced by IRR. Conclusion: We hypothesize that the stimulation of PPAR-\u03b3 expression and interference with miR-17-5p activated Wnt\u2013\u03b2-catenin signaling mediates the antifibrotic properties of Dios. <\/jats:p>","DOI":"10.1139\/bcb-2016-0142","type":"journal-article","created":{"date-parts":[[2016,11,21]],"date-time":"2016-11-21T23:08:06Z","timestamp":1479769686000},"page":"400-414","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":46,"title":["Diosmin attenuates radiation-induced hepatic fibrosis by boosting PPAR-\u03b3 expression and hampering miR-17-5p-activated canonical Wnt\u2013\u03b2-catenin signaling"],"prefix":"10.1139","volume":"95","author":[{"given":"Hesham Farouk","family":"Hasan","sequence":"first","affiliation":[{"name":"Radiation Biology Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, PO Box 29, Nasr City, Cairo, Egypt."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed Khairy","family":"Abdel-Rafei","sequence":"additional","affiliation":[{"name":"Radiation Biology Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, PO Box 29, Nasr City, Cairo, Egypt."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shereen Mohamed","family":"Galal","sequence":"additional","affiliation":[{"name":"Health Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, PO Box 29, Nasr City, Cairo, Egypt."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","unstructured":"Abdel-Rafei, M., Amin, M.M., and Hasan, H.F. 2016. 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