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The purpose of this study was to investigate the protective effects of HPN on hypoxia-induced damage in PC12 cells. It was shown that HPN significantly attenuated hypoxia-induced loss of cell viability, release of lactate dehydrogenase (LDH), and morphological changes in PC12 cells. Moreover, hypoxic PC12 cells had increased levels of reactive oxygen species (ROS), malondialdehyde (MDA), and expression of HIF-1\u03b1 and VEGF, but had reduced levels of superoxide dismutase (SOD) and catalase (CAT), and HPN reversed these changes. HPN also inhibited hypoxia-induced cell apoptosis via suppressing the expression of Bax, cytochrome c, and caspase-3, and inducing the expression of Bcl-2. These results indicate that the protective effects of HPN on hypoxia-induced damage in PC12 cells is associated with the suppression of hypoxia-induced oxidative stress and cell apoptosis. HPN could be a promising candidate for the development of a novel neuroprotective agent. <\/jats:p>","DOI":"10.1139\/bcb-2019-0269","type":"journal-article","created":{"date-parts":[[2019,11,5]],"date-time":"2019-11-05T21:55:10Z","timestamp":1572990910000},"page":"345-353","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Protective effect of nitronyl nitroxide against hypoxia-induced damage in PC12 cells"],"prefix":"10.1139","volume":"98","author":[{"given":"Hongbo","family":"Luo","sequence":"first","affiliation":[{"name":"Department of Neurology, the Fifth Affiliated Hospital of Zunyi Medical University, Zhuhai, Guangdong, 519000, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, the 940th Hospital of Joint Logistics Support force of PLA, 333 Binhenan Road, Qilihe district Lanzhou, Gansu, 730050, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, the 940th Hospital of Joint Logistics Support force of PLA, 333 Binhenan Road, Qilihe district Lanzhou, Gansu, 730050, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiping","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, the 940th Hospital of Joint Logistics Support force of PLA, 333 Binhenan Road, Qilihe district Lanzhou, Gansu, 730050, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengping","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, the 940th Hospital of Joint Logistics Support force of PLA, 333 Binhenan Road, Qilihe district Lanzhou, Gansu, 730050, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linlin","family":"Jing","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, the 940th Hospital of Joint Logistics Support force of PLA, 333 Binhenan Road, Qilihe district Lanzhou, Gansu, 730050, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2016.02.030"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1021\/cn300143m"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1021\/bi00054a013"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2005.11.006"},{"key":"refg5\/ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2009.06.027"},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1016\/S0028-3908(02)00180-6"},{"key":"refg7\/ref7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.20.11715"},{"key":"refg8\/ref8","doi-asserted-by":"publisher","DOI":"10.1039\/c4fo00948g"},{"key":"refg9\/ref9","doi-asserted-by":"publisher","DOI":"10.1186\/1423-0127-21-46"},{"key":"refg10\/ref10","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.290020305"},{"key":"refg11\/ref11","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b12-00854"},{"key":"refg12\/ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2017.05.053"},{"key":"refg13\/ref13","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(96)00059-9"},{"key":"refg14\/ref14","doi-asserted-by":"crossref","unstructured":"G\u00f6rlach, A. 2014. 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