{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T05:24:27Z","timestamp":1788672267460,"version":"build-2803163510"},"reference-count":26,"publisher":"Canadian Science Publishing","issue":"4","license":[{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Biochem. Cell Biol."],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:p> The natural product pateamineA (PatA) is a highly potent antiproliferative agent. PatA and the simplified analog desmethyl, desamino pateamineA (DMDAPatA) have exhibited cytotoxicity selective for rapidly proliferating cells, and have been shown to inhibit cap-dependent translation initiation through binding to eIF4A (eukaryotic initiation factor 4A) of the eIF4F complex. PatA and DMDAPatA are both known to stimulate the RNA-dependent ATPase, and ATP-dependent RNA helicase activities of eIF4A. The impact of other eIF4F components, eIF4E and eIF4G, on DMDAPatA action were investigated in vitro and in cultured mammalian cells. The perturbation of the eIF4A\u2013eIF4G association was found to be eIF4E- and mRNA cap-dependent. An inhibitory effect on helicase activity of eIF4A was observed when it was part of a complex that mimicked the eIF4F complex. We propose a model of action for DMDAPatA (and by supposition PatA) where the cellular activity of the compound is dependent on an \u201cactive\u201d eIF4F complex. <\/jats:p>","DOI":"10.1139\/bcb-2019-0307","type":"journal-article","created":{"date-parts":[[2020,2,2]],"date-time":"2020-02-02T21:32:52Z","timestamp":1580679172000},"page":"502-510","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":12,"title":["Investigation of the mechanism of action of a potent pateamine A analog, des-methyl, des-amino pateamine A (DMDAPatA)"],"prefix":"10.1139","volume":"98","author":[{"given":"Sai Shilpa","family":"Kommaraju","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, St. John\u2019s University, 8000 Utopia Parkway, Queens, NY 11439, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julieta","family":"Aulicino","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, St. John\u2019s University, 8000 Utopia Parkway, Queens, NY 11439, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shruthi","family":"Gobbooru","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, St. John\u2019s University, 8000 Utopia Parkway, Queens, NY 11439, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"Natural Products LINCHPIN Laboratory and Department of Chemistry, Texas A&M University, P.O. Box 30012, College Station, TX 77843, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingzhao","family":"Zhu","sequence":"additional","affiliation":[{"name":"Natural Products LINCHPIN Laboratory and Department of Chemistry, Texas A&M University, P.O. Box 30012, College Station, TX 77843, USA."},{"name":"Department of Chemistry and Biochemistry and the CPRIT Synthesis and Drug Lead Discovery Laboratory, Baylor University, One Bear Place #97348, Waco, TX 76798, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Romo","sequence":"additional","affiliation":[{"name":"Natural Products LINCHPIN Laboratory and Department of Chemistry, Texas A&M University, P.O. 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