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Cell Biol."],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:p> This study explored the synergistic effects of autophagy inhibitors combined with cisplatin against cisplatin-resistant nasopharyngeal cancer cells by treating HNE-1 and cisplatin (diamminedichloroplatinum; DDP)-resistant HNE1\/DDP nasopharyngeal cancer cell lines with DDP, autophagy inhibitors, or a combination of autophagy inhibitors and DDP. Cell viability was determined via MTT (colorimetric) and colony-forming assays, and the rate of apoptosis was determined using propidium iodide (PI) and annexin V double-staining. The expressions of proteins were determined by Western blotting. For our in-vivo studies, a murine xenograft model was established to evaluate the anti-tumor effects of the combination of autophagy inhibitor and DDP. The results showed that treatment with DDP increased the expressions of ATP-binding cassette sub-family B member 1 (ABCB1), ATP Binding Cassette Subfamily C Member 1 (ABCC1), and P-glycoprotein 1 (P-gp) in the HNE1\/DDP cell lines. Treatment with chloroquine decreased the expression levels of ABCB1, ABCC1, and P-gp, and increased the formation of LC3-II and the expression levels of p62 in the HNE1\/DDP cells. Additionally, the combination of autophagy inhibitors and DDP produced a synergistic effect on DDP-induced cell death and apoptosis. Furthermore, the combination of the autophagy inhibitor and DDP showed significant anti-tumor effects in the xenograft mouse model. In summary, autophagy inhibitors show synergistic anti-tumor effects with DDP in vitro against DDP-resistant nasopharyngeal cancer cells and in vivo in our xenograft murine model. <\/jats:p>","DOI":"10.1139\/bcb-2020-0283","type":"journal-article","created":{"date-parts":[[2021,5,26]],"date-time":"2021-05-26T17:54:36Z","timestamp":1622051676000},"page":"322-329","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Synergistic effects of autophagy inhibitors combined with cisplatin against cisplatin-resistant nasopharyngeal cancer cells"],"prefix":"10.1139","volume":"99","author":[{"given":"Wei","family":"Yin","sequence":"first","affiliation":[{"name":"Hangzhou Cancer Hospital, No. 34 Yanguanxiang, Hangzhou Zhejiang 310002, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianfeng","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery, Jiande Second People\u2019s Hospital, No. 223 Zongfu Street, Meicheng Town, Jiande Zheijang 311064, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanjiao","family":"Mao","sequence":"additional","affiliation":[{"name":"Hangzhou Cancer Hospital, No. 34 Yanguanxiang, Hangzhou Zhejiang 310002, China."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anl.2018.10.013"},{"key":"refg2\/ref2","unstructured":"Almomani, M.H., Zulfiqar, H., and Nagalli, S. 2020. 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