{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T20:47:52Z","timestamp":1789505272966,"version":"build-2803163510"},"reference-count":32,"publisher":"Canadian Science Publishing","issue":"4","license":[{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Biochem. Cell Biol."],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:p> The microRNAs miR-17-5p and miR-20a-5p play important roles on angiogenesis; however, it is arguable whether they regulate the formation of retinal blood vessels in retinopathy of prematurity (ROP). We used a mouse model of oxygen-induced retinopathy (OIR) to simulate the development of retinas in mice suffering from ROP, and the expression levels of miR-20a-5p, miR-17-5p, hypoxia-inducible factor 1-alpha (HIF-1\u03b1), and vascular endothelial growth factor (VEGF) were measured by RT-qPCR and Western blotting. Cell proliferation, apoptosis, and angiogenesis in the OIR model mice were measured using MTT assays, flow cytometry, and Matrigel assays, respectively. The interaction between HIF-1\u03b1\/VEGF and miR-20a-5p\/miR-17-5p were further validated using dual-luciferase reporter assays, biotin-labeled RNA-pulldown, and RNA immunoprecipitation (RIP) assays. In our OIR model, retinal angiogenesis in the mice was associated with down-regulation of miR-20a-5p and miR-17-5p, as well as up-regulation of HIF-1\u03b1 and VEGF. In addition, the miR-20a-5p and miR-17-5p inhibited cell proliferation and angiogenesis through regulating HIF-1\u03b1 and VEGF in the retinal cells of the OIR model mice. Moreover, it was found that miR-20a-5p and miR-17-5p bind to HIF-1\u03b1 and VEGF at the 3\u2032UTR, and there was a combined effect between miR-20a-5p and miR-17-5p on the regulation of HIF-1\u03b1 and VEGF. It is worth noting that miR-17-5p and miR-20a-5p can preferentially regulate HIF-1\u03b1, then act on VEGF, thereby affecting the angiogenesis associated with ROP. <\/jats:p>","DOI":"10.1139\/bcb-2020-0357","type":"journal-article","created":{"date-parts":[[2021,7,28]],"date-time":"2021-07-28T18:00:02Z","timestamp":1627495202000},"page":"414-423","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["<i>VEGF<\/i>-mediated angiogenesis in retinopathy of prematurity is co-regulated by miR-17-5p and miR-20a-5p"],"prefix":"10.1139","volume":"99","author":[{"given":"Yan","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Hunan Children\u2019s Hospital, Changsha 410007, Hunan Province, P.R. 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