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We used uric acid (UA), a potent antioxidant found in biological fluids, to protect human red blood cells (RBC) and lymphocytes against Hg-mediated cell, organelle, and genotoxicity. RBCs were incubated with mercuric chloride (HgCl<jats:sub>2<\/jats:sub>), an Hg(II) compound, either alone or in the presence of UA. Incubation of RBCs with only HgCl<jats:sub>2<\/jats:sub> increased the production of nitrogen and oxygen radical species, enhanced methemoglobin levels, heme degradation, free ferrous iron, oxidation of proteins and membrane lipids, and reduced the antioxidant capacity of cells. UA enhanced the antioxidant capacity of RBCs and restored metabolic, plasma membrane-bound, and antioxidant enzyme activities. Scanning electron microscopy showed that UA prevented HgCl<jats:sub>2<\/jats:sub>-mediated morphological changes in RBCs. HgCl<jats:sub>2<\/jats:sub> dissipated the mitochondrial membrane potential and increased lysosomal membrane damage in lymphocytes, but UA pre-treatment attenuated these effects. Genotoxicity analysis by comet assay showed that UA protected lymphocyte DNA from HgCl<jats:sub>2<\/jats:sub>-induced damage. Importantly, UA itself did not exhibit any deleterious effects on RBCs or lymphocytes. Thus, UA protects human blood cells from Hg(II)-mediated oxidative damage, reducing the harmful effects of this extremely toxic metal. We suggest that UA has a similar protective role in plasma against heavy metal toxicity. <\/jats:p>","DOI":"10.1139\/bcb-2021-0229","type":"journal-article","created":{"date-parts":[[2021,10,15]],"date-time":"2021-10-15T21:41:14Z","timestamp":1634334074000},"page":"45-58","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Attenuation of Hg(II)-induced cellular and DNA damage in human blood cells by uric acid"],"prefix":"10.1139","volume":"100","author":[{"given":"Shahbaz","family":"Ahmad","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India."},{"name":"Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neda","family":"Tufail","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India."},{"name":"Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazia","family":"Parveen","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India."},{"name":"Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riaz","family":"Mahmood","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India."},{"name":"Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(84)05016-3"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-018-04062-5"},{"key":"refg3\/ref3","first-page":"1243","volume":"36","author":"Aijafari A.A.","year":"1995","journal-title":"Biochem. 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