{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T11:04:22Z","timestamp":1777460662991,"version":"3.51.4"},"reference-count":68,"publisher":"Canadian Science Publishing","issue":"1","license":[{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Biochem. Cell Biol."],"published-print":{"date-parts":[[2023,2,1]]},"abstract":"<jats:p> Myocardial regenerative strategies are promising where the choice of ideal cell population is crucial for successful translational applications. Herein, we explored the regenerative\/repair responses of infarct zone cardiac fibroblast(s) (CF) by unveiling their phenotype heterogeneity at single-cell resolution. CF were isolated from the infarct zone of Yucatan miniswine that suffered myocardial infarction, cultured under simulated ischemic and reperfusion, and grouped into control, ischemia, and ischemia\/reperfusion. The single-cell RNA sequencing analysis revealed 19 unique cell clusters suggesting distinct subpopulations. The status of gene expression (log2 fold change (log2 FC)\u00a0&gt;\u00a02 and log2 FC &lt; \u22122) was used to define the characteristics of each cluster unveiling with diverse features, including the pro-survival\/cardioprotective (Clusters 1, 3, 5, 9, and 18), vasculoprotective (Clusters 2 and 5), anti-inflammatory (Clusters 4 and 17), proliferative (Clusters 4 and 5), nonproliferative (Clusters 6, 8, 11, 16, 17, and 18), proinflammatory (Cluster 6), profibrotic\/pathologic (Clusters 8 and 19), antihypertrophic (Clusters 8 and 10), extracellular matrix restorative (Clusters 9 and 12), angiogenic (Cluster 16), and normal (Clusters 7 and 15) phenotypes. Further understanding of these unique phenotypes of CF will provide significant translational opportunities for myocardial regeneration and cardiac management. <\/jats:p>","DOI":"10.1139\/bcb-2022-0229","type":"journal-article","created":{"date-parts":[[2022,11,3]],"date-time":"2022-11-03T21:34:46Z","timestamp":1667511286000},"page":"12-51","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Single-cell genomics illustrates heterogeneous phenotypes of myocardial fibroblasts under ischemic insults"],"prefix":"10.1139","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7285-9808","authenticated-orcid":false,"given":"Finosh G.","family":"Thankam","sequence":"first","affiliation":[{"name":"Department of Translational Research, Western University of Health Sciences","place":["Pomona, USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vy","family":"La","sequence":"additional","affiliation":[{"name":"Department of Translational Research, Western University of Health Sciences","place":["Pomona, USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Devendra K.","family":"Agrawal","sequence":"additional","affiliation":[{"name":"Department of Translational Research, Western University of Health Sciences","place":["Pomona, USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1002\/ehf2.12560"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.2174\/138920210793360907"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000035652.71915.00"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-64097-y"},{"key":"refg5\/ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2019.09.032"},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1358\/dot.2012.48.7.1811719"},{"key":"refg7\/ref7","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222313116"},{"key":"refg8\/ref8","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2021.599928"},{"key":"refg9\/ref9","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23010234"},{"key":"refg10\/ref10","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22041693"},{"key":"refg11\/ref11","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.43882"},{"key":"refg12\/ref12","doi-asserted-by":"publisher","DOI":"10.1111\/febs.16319"},{"key":"refg13\/ref13","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2103676118"},{"key":"refg14\/ref14","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.120.019338"},{"key":"refg15\/ref15","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvac037"},{"issue":"8","key":"refg16\/ref16","first-page":"8786","volume":"8","author":"Hashmi S.","year":"2015","journal-title":"Int. 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