{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,7]],"date-time":"2024-07-07T07:01:23Z","timestamp":1720335683056},"reference-count":15,"publisher":"Canadian Science Publishing","issue":"3","license":[{"start":{"date-parts":[[2014,3,1]],"date-time":"2014-03-01T00:00:00Z","timestamp":1393632000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:p>In a previous study, we described 2 forms of cyclic enterobacterial common antigen (ECA<jats:sub>CYC<\/jats:sub>), a tetramer and a pentamer, from Escherichia coli O157. ECA<jats:sub>CYC<\/jats:sub>is present in several representatives of the Enterobacteriaceae. To date, functional studies on ECA<jats:sub>CYC<\/jats:sub>are sparse. Cyclic oligosaccharides in other bacteria, like the cyclic \u03b2-glucans in Rhizobiaceae, represent microbe-associated molecular patterns involved in host\u2013bacteria interaction. This observation determined the aim of the present study: to test whether the tetrameric and pentameric ECA<jats:sub>CYC<\/jats:sub>from E. coli O157 can be recognised by host humoral and cellular mechanisms. ELISA tests designed to compare the 2 ECA<jats:sub>CYC<\/jats:sub>with the O157 lipopolysaccharide showed that both ECA<jats:sub>CYC<\/jats:sub>were not recognised by polyclonal anti-O157 serum but were good ligands for mannan-binding lectin. The lectin had a higher affinity for the tetramer than the pentamer. ECA<jats:sub>CYC<\/jats:sub>deposited more C3b than did the lipopolysaccharide. To examine the interactions with human circulating neutrophils, the antigens were loaded onto fluorescent latex beads and applied in a phagocytosis experiment. Spheres coated with the 2 ECA<jats:sub>CYC<\/jats:sub>occasionally adhered to phagocyte surfaces but, unlike O157-loaded spheres, failed to induce free-radical release. The results show that the 2 ECA<jats:sub>CYC<\/jats:sub>represent microbe-associated molecular patterns recognised by host humoral non-self-recognition mechanisms.<\/jats:p>","DOI":"10.1139\/cjm-2013-0697","type":"journal-article","created":{"date-parts":[[2014,1,13]],"date-time":"2014-01-13T14:49:48Z","timestamp":1389624588000},"page":"173-176","source":"Crossref","is-referenced-by-count":1,"title":["Cyclic enterobacterial common antigens from<i>Escherichia coli<\/i>O157 as microbe-associated molecular patterns"],"prefix":"10.1139","volume":"60","author":[{"given":"Tsvetelina S.","family":"Paunova-Krasteva","sequence":"first","affiliation":[{"name":"The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. 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