{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:16:18Z","timestamp":1791317778637,"version":"4.3.1"},"reference-count":30,"publisher":"Canadian Science Publishing","issue":"6","license":[{"start":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T00:00:00Z","timestamp":1464739200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:p> Most vaccines for protection against Mycoplasma bovis disease are made of bacterins, and they offer varying degrees of protection. Our focus is on the development of a subunit-based protective vaccine, and to that end, we have identified 10 novel vaccine candidates. After formulation of these candidates with TriAdj, an experimental tri-component novel vaccine adjuvant developed at VIDO\u2013InterVac, we measured humoral and cell-mediated immune responses in vaccinated animals. In addition, we compared the immune responses after formulation with TriAdj with the responses measured in animals vaccinated with a mix of a commercial adjuvant (Emulsigen\u2122) and 2 of the components of the TriAdj, namely polyinosinic:polycytidylic acid (poly I:C) and the cationic innate defense regulator (IDR) peptide 1002 (VQRWLIVWRIRK). In this latter trial, we detected significant IgG1 humoral immune responses to 8 out of 10 M. bovis proteins, and IgG2 responses to 7 out of 10 proteins. Thus, we concluded that the commercial adjuvant formulated with poly I:C and the IDR peptide 1002 is the best formulation for the experimental vaccine. <\/jats:p>","DOI":"10.1139\/cjm-2015-0762","type":"journal-article","created":{"date-parts":[[2016,2,3]],"date-time":"2016-02-03T22:49:27Z","timestamp":1454539767000},"page":"492-504","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":17,"title":["Immune responses to <i>Mycoplasma bovis<\/i> proteins formulated with different adjuvants"],"prefix":"10.1139","volume":"62","author":[{"given":"Tracy","family":"Prysliak","sequence":"first","affiliation":[{"name":"Vaccine and Infectious Disease Organization \u2013 International Vaccine Centre (VIDO\u2013InterVac), 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada."},{"name":"Vaccine and Infectious Disease Organization \u2013 International Vaccine Centre (VIDO\u2013InterVac), 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose","family":"Perez-Casal","sequence":"additional","affiliation":[{"name":"Vaccine and Infectious Disease Organization \u2013 International Vaccine Centre (VIDO\u2013InterVac), 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada."},{"name":"Vaccine and Infectious Disease Organization \u2013 International Vaccine Centre (VIDO\u2013InterVac), 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2007.03.008"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2010.03.016"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvfa.2010.03.003"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(80)90015-2"},{"key":"refg5\/ref5","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.062570-0"},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.07.009"},{"key":"refg7\/ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2010.12.005"},{"key":"refg8\/ref8","doi-asserted-by":"publisher","DOI":"10.1136\/vr.121.16.372"},{"key":"refg9\/ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.08.037"},{"key":"refg10\/ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.02.100"},{"key":"refg11\/ref11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2011.0750.x"},{"key":"refg12\/ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.12.055"},{"key":"refg13\/ref13","doi-asserted-by":"publisher","DOI":"10.1136\/vr.d2468"},{"key":"refg14\/ref14","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5288(02)00155-8"},{"issue":"29","key":"refg15\/ref15","doi-asserted-by":"crossref","first-page":"3569","DOI":"10.1016\/S0264-410X(02)00340-7","volume":"20","author":"Nicholas R.A.","year":"2002","journal-title":"Vaccine"},{"key":"refg16\/ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2007.05.009"},{"issue":"11","key":"refg17\/ref17","first-page":"1195","volume":"52","author":"Prysliak T.","year":"2011","journal-title":"Can. Vet. J."},{"key":"refg18\/ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2012.12.001"},{"key":"refg19\/ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2008.10.008"},{"key":"refg20\/ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2005.05.007"},{"key":"refg21\/ref21","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(93)90097-Q"},{"issue":"2","key":"refg22\/ref22","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1128\/IAI.68.2.680-687.2000","volume":"68","author":"Sachse K.","year":"2000","journal-title":"Infection and Immunity"},{"key":"refg23\/ref23","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00158-X"},{"issue":"1","key":"refg24\/ref24","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0378-1119(93)90171-X","volume":"134","author":"Smiley B.K.","year":"1993","journal-title":"Gene."},{"issue":"15","key":"refg25\/ref25","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1136\/vr.121.15.342","volume":"121","author":"Stott E.J.","year":"1987","journal-title":"Vet. Rec."},{"key":"refg26\/ref26","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2427(90)90063-X"},{"key":"refg28\/ref28","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(03)00056-4"},{"key":"refg29\/ref29","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.01.063"},{"key":"refg27\/ref27","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00707-10"},{"key":"refg30\/ref30","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2013.12.004"}],"container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdnsciencepub.com\/doi\/full-xml\/10.1139\/cjm-2015-0762","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdnsciencepub.com\/doi\/pdf\/10.1139\/cjm-2015-0762","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T17:56:50Z","timestamp":1751479010000},"score":1,"resource":{"primary":{"URL":"https:\/\/cdnsciencepub.com\/doi\/10.1139\/cjm-2015-0762"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6]]},"references-count":30,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,6]]}},"alternative-id":["10.1139\/cjm-2015-0762"],"URL":"https:\/\/doi.org\/10.1139\/cjm-2015-0762","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,6]]},"assertion":[{"value":"2015-11-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-01-13","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-01-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-02-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}