{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T15:05:46Z","timestamp":1759935946146,"version":"3.41.0"},"reference-count":28,"publisher":"Canadian Science Publishing","issue":"3","license":[{"start":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T00:00:00Z","timestamp":1456790400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:p> Mycoplasma bovis is a pathogen globally affecting cattle and bison herds, causing pneumonia, arthritis, mastitis, abortions, and other symptoms, leading to huge economic losses. Many studies have been done regarding the antimicrobial susceptibility of M. bovis isolated from cattle, but no such study is available for isolates recovered from bison. For the first time, in vitro susceptibilities of 40 M. bovis clinical isolates collected from bison herds in Canada are reported here. Minimal inhibitory concentration (MIC) values were determined using Sensititre\u00ae plates. The most effective MIC<jats:sub>50<\/jats:sub> and MIC<jats:sub>90<\/jats:sub> were for spectinomycin (1 and &gt;64 \u03bcg\/mL), tiamulin (1 and &gt;32 \u03bcg\/mL), and tulathromycin (16 and 64 \u03bcg\/mL), whereas tetracyclines, fluoroquinolones, and florfenicol failed to inhibit growth of M. bovis bison isolates. Isolates were nonsusceptible to tetracyclines (100%), fluoroquinolones (97.5%), and tilmicosin (100%), whereas the highest susceptibility of bison clinical isolates was seen with spectinomycin (95%) and tulathromycin (67.5%). Two lung isolates (Mb283 and 348) were found resistant to both spectinomycin and tulathromycin. These results show a marked difference in antimicrobial susceptibility of bison isolates as compared with previously reported and laboratory reference cattle isolates, emphasizing the necessity of testing antimicrobial susceptibility of M. bovis bison isolates and to generate better therapeutic regime for improved recovery chances for infected bison herds across North America. <\/jats:p>","DOI":"10.1139\/cjm-2015-0763","type":"journal-article","created":{"date-parts":[[2015,12,18]],"date-time":"2015-12-18T22:39:14Z","timestamp":1450478354000},"page":"272-278","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["In vitro antimicrobial susceptibility of <i>Mycoplasma bovis<\/i> clinical isolates recovered from bison (<i>Bison bison<\/i>)"],"prefix":"10.1139","volume":"62","author":[{"given":"Muhammad","family":"Suleman","sequence":"first","affiliation":[{"name":"Vaccine and Infectious Disease Organization \u2013 International Vaccine Centre, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tracy","family":"Prysliak","sequence":"additional","affiliation":[{"name":"Vaccine and Infectious Disease Organization \u2013 International Vaccine Centre, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire","family":"Windeyer","sequence":"additional","affiliation":[{"name":"Department of Production Animal Health, University of Calgary, Faculty of Veterinary Medicine, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose","family":"Perez-Casal","sequence":"additional","affiliation":[{"name":"Vaccine and Infectious Disease Organization \u2013 International Vaccine Centre, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03876-14"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2007.03.008"},{"journal-title":"J. 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