{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T16:17:29Z","timestamp":1764433049088,"version":"3.41.0"},"reference-count":41,"publisher":"Canadian Science Publishing","issue":"10","license":[{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:p> This study investigated bacteria from soil samples to (i) determine the main bacterial genera and species having resistance to carbapenem and other \u03b2-lactams and (ii) establish if the mechanism of resistance was due to the production of metallo-\u03b2-lactamases. The isolates were characterized by PCR for metallo-\u03b2-lactamases and integrons, by antimicrobial susceptibility testing, and by sequencing. The antimicrobial profile of 40 imipenem-resistant Gram-positive soil isolates from all Brazilian regions demonstrated that 31 (77.5%) of them were multidrug resistant. Among the 40 isolates, 19 presented the bla<jats:sub>VIM<\/jats:sub> gene and class 1 integrons by PCR. Six of the 19 isolates were identified as Paenibacillus sp., 12 as Bacillus sp., and just 1 was classified as Staphylococcus sp., by sequencing of the 16S rRNA gene. These results suggest that bacteria from soil can act as a source of bla<jats:sub>VIM-1<\/jats:sub> genes, representing a threat to public health. <\/jats:p>","DOI":"10.1139\/cjm-2015-0787","type":"journal-article","created":{"date-parts":[[2016,5,31]],"date-time":"2016-05-31T21:46:03Z","timestamp":1464731163000},"page":"820-826","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["High prevalence of <i>bla<\/i><sub>VIM-1<\/sub> gene in bacteria from Brazilian soil"],"prefix":"10.1139","volume":"62","author":[{"given":"Andr\u00e9","family":"Pitondo-Silva","sequence":"first","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto \u2013 Universidade de S\u00e3o Paulo \u2013 Ribeir\u00e3o Preto, Brazil."},{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto \u2013 Universidade de S\u00e3o Paulo \u2013 Ribeir\u00e3o Preto, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz Baptistella","family":"Devechio","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto \u2013 Universidade de S\u00e3o Paulo \u2013 Ribeir\u00e3o Preto, Brazil."},{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto \u2013 Universidade de S\u00e3o Paulo \u2013 Ribeir\u00e3o Preto, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00e9ssica Aparecida Silva","family":"Moretto","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto \u2013 Universidade de S\u00e3o Paulo \u2013 Ribeir\u00e3o Preto, Brazil."},{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto \u2013 Universidade de S\u00e3o Paulo \u2013 Ribeir\u00e3o Preto, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eliana Guedes","family":"Stehling","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto \u2013 Universidade de S\u00e3o Paulo \u2013 Ribeir\u00e3o Preto, Brazil."},{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto \u2013 Universidade de S\u00e3o Paulo \u2013 Ribeir\u00e3o Preto, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.12.4654-4661.2004"},{"key":"refg2\/ref2","unstructured":"Clinical and Laboratory Standards Institute. 2013. 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