{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T18:03:35Z","timestamp":1761156215709,"version":"3.41.0"},"reference-count":26,"publisher":"Canadian Science Publishing","issue":"4","license":[{"start":{"date-parts":[[2017,4,1]],"date-time":"2017-04-01T00:00:00Z","timestamp":1491004800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:p> Gastroenteritis is a common disease in children. It is characterized by diarrhea, vomiting, abdominal pain, and fever. Sapovirus (SaV) is a causative agent of acute gastroenteritis, but it causes milder illness than do rotavirus and norovirus. There is high variability in the analytical performance of quantitative PCR-based assays among clinical laboratories. This study developed a reverse transcription real-time PCR method to detect SaV in fecal specimens collected from children under 5-years-old with acute gastroenteritis. Of 137 episodes of acute gastroenteritis, 15 (10.9%) were associated with SaV genomic detection, with a median viral load of 6.6(log<jats:sub>10<\/jats:sub>) \u00b1 7.1(log<jats:sub>10<\/jats:sub>) genomes\/mg fecal specimens. There was a significant difference in detection rate between males and females (9.48% (13\/15) vs. 1.46% (2\/15), p = 0.0232). Among the 15 SaV-positive cases, 6 were also positive for rotavirus. Viral RNA recovery rate ranged from 46% to 77% in the manual RNAzol protocol and from 31% to 90% in the automated Maxwell protocol. We also studied whether human genomic DNA influences the sensitivity of the assay: its presence caused a decrease in PCR sensitivity. The development of a laboratory-designed real-time PCR TaqMan assay for quantitative detection of SaV and the optimization and standardization of this assay, using stools of children with acute gastroenteritis, are described. <\/jats:p>","DOI":"10.1139\/cjm-2016-0482","type":"journal-article","created":{"date-parts":[[2016,12,2]],"date-time":"2016-12-02T22:35:17Z","timestamp":1480718117000},"page":"296-302","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":12,"title":["Development of a quantitative real-time PCR assay for sapovirus in children under 5-years-old in Regina Margherita Hospital of Turin, Italy"],"prefix":"10.1139","volume":"63","author":[{"given":"Massimiliano","family":"Bergallo","sequence":"first","affiliation":[{"name":"Department of Public Health and Pediatric Sciences, University of Turin, Medical School, 10136 Turin, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilaria","family":"Galliano","sequence":"additional","affiliation":[{"name":"Department of Public Health and Pediatric Sciences, University of Turin, Medical School, 10136 Turin, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Montanari","sequence":"additional","affiliation":[{"name":"Department of Public Health and Pediatric Sciences, University of Turin, Medical School, 10136 Turin, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina Rosa","family":"Brusin","sequence":"additional","affiliation":[{"name":"Struttura Complessa, Pediatric University Regina Margherita Hospital, Piazza Polonia 94, 1016 Turin, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Serena","family":"Finotti","sequence":"additional","affiliation":[{"name":"Department of Public Health and Pediatric Sciences, University of Turin, Medical School, 10136 Turin, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Paderi","sequence":"additional","affiliation":[{"name":"Department of Public Health and Pediatric Sciences, University of Turin, Medical School, 10136 Turin, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clara","family":"Gabiano","sequence":"additional","affiliation":[{"name":"Struttura Complessa, Pediatric University Regina Margherita Hospital, Piazza Polonia 94, 1016 Turin, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-007-0290-8"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2015.04.018"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0098201"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890040402"},{"key":"refg5\/ref5","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-014-2135-6"},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20488"},{"key":"refg7\/ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2009.07.008"},{"key":"refg8\/ref8","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.034611-0"},{"key":"refg9\/ref9","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00915-06"},{"key":"refg10\/ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2015.03.039"},{"key":"refg11\/ref11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2004.00699.x"},{"key":"refg12\/ref12","doi-asserted-by":"publisher","DOI":"10.1111\/1348-0421.12107"},{"key":"refg13\/ref13","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1007\/s00705-011-1161-2","volume":"157","author":"Oka T.","year":"2012","journal-title":"Arch. 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