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Compared with other M types, serotype M3 has a predominant role in lethal infections and demonstrates epidemic behaviors, including streptococcal toxic shock syndrome, bacteremia, and necrotizing fasciitis. Traditional methods for M typing are time-consuming, tedious, contradictory, and generally restricted to reference laboratories. Therefore, development of a new M-typing technique is needed. Aptamers with the ability to detect their target with a high degree of accuracy and specificity can be ideal candidates for specific M-typing of Streptococcus pyogenes. In this study DNA aptamers with a high binding affinity towards S. pyogenes serotype M3 were selected through 12 iterative rounds of the Systematic Evolution of Ligands by EXponential (SELEX) enrichment procedure using live cells as a target. We monitored the progress of the SELEX procedure by flow cytometry analysis. Of several aptamer sequences analyzed, 12L18A showed the highest binding efficiency towards S. pyogenes type M3, with an apparent dissociation constant (K<jats:sub>d<\/jats:sub>) of 7.47 \u00b1 1.72 pmol\/L being the lowest. Therefore the isolated aptamer can be used in any tool, such as a biosensor, for the detection of S. pyogenes and can be used in the development of a novel M-typing system. <\/jats:p>","DOI":"10.1139\/cjm-2016-0495","type":"journal-article","created":{"date-parts":[[2016,10,14]],"date-time":"2016-10-14T21:37:36Z","timestamp":1476481056000},"page":"160-168","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":11,"title":["Development of a DNA aptamer that binds specifically to group A <i>Streptococcus<\/i> serotype M3"],"prefix":"10.1139","volume":"63","author":[{"given":"Hanif","family":"Alfavian","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed Latif","family":"Mousavi Gargari","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samaneh","family":"Rasoulinejad","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arvin","family":"Medhat","sequence":"additional","affiliation":[{"name":"GENEXIR Biopharma, a knowledge-based company at Pasteur Institute of Iran Health Technology Park, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2015.05.050"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","unstructured":"Alves Ferreira-Bravo, I., Cozens, C., Holliger, P., and DeStefano, J.J. 2015. 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