{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T23:42:36Z","timestamp":1791848556836,"version":"4.3.4"},"reference-count":24,"publisher":"Canadian Science Publishing","issue":"8","license":[{"start":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T00:00:00Z","timestamp":1501545600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:p> Brucella is Gram-negative intracellular bacterial pathogen that infects humans and animals and contributes to great economic losses in developing countries. Presently, live attenuated Brucella vaccines (Brucella melitensis M5-90) are the most effective means of brucellosis control and prevention in animals. However, these vaccines have several drawbacks, such as an inability to distinguish between a natural infection and immunization and an association with abortions in pregnant animals. Therefore, this study constructed a Brucella M5-90\u0394bp26 mutant and evaluated its virulence. The survival of the M5-90\u0394bp26 mutant was attenuated in human placenta trophoblastic 8 cells (HPT-8 cells) and in BALB\/c mice, with a high immunoprotectivity noted in mice. Furthermore, safety tests showed that the M5-90\u0394bp26 mutant was less virulent than the M5-90 vaccine strain. Additionally, an indirect enzyme-linked immunosorbent assay (ELISA) screening was shown to detect the presence of Brucella protein 26 (BP26) with high sensitivity, with M5-90\u0394bp26 inoculation accompanied with a lack of BP26 expression, and was further confirmed by western blotting. Together, the M5-90\u0394bp26 mutant and the indirect ELISA can be employed to distinguish vaccinated livestock from infected animals. <\/jats:p>","DOI":"10.1139\/cjm-2017-0179","type":"journal-article","created":{"date-parts":[[2017,5,8]],"date-time":"2017-05-08T21:38:21Z","timestamp":1494279501000},"page":"719-729","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":22,"title":["<i>Brucella melitensis<\/i> M5-90\u0394bp26 as a potential live vaccine that allows for the distinction between natural infection and immunization"],"prefix":"10.1139","volume":"63","author":[{"given":"Tiansen","family":"Li","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, 832000, Shihezi, Xinjiang, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhixia","family":"Tong","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, 832000, Shihezi, Xinjiang, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiling","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Life Science, Shihezi University, 832000, Shihezi, Xinjiang, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyan","family":"Tang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, 832000, Shihezi, Xinjiang, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, 832000, Shihezi, Xinjiang, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuangfu","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, 832000, Shihezi, Xinjiang, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"issue":"4","key":"refg1\/ref1","first-page":"350","volume":"18","author":"Amani J.","year":"2015","journal-title":"Iran. 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