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Overexpression of MexXY pump was observed in all of the isolates tested. Analysis of regulatory genes that control the expression of these 4 efflux pumps revealed a number of previously uncharacterized mutations. Our work shows that MexXY pump overexpression is common in cystic fibrosis isolates and could be contributing to their reduced aminoglycoside susceptibility. Further, we also identified novel mutations in the regulatory genes of the 4 abovementioned Resistance\u2013Nodulation\u2013Division superfamily pumps that may be involved in the overexpression of these pumps. <\/jats:p>","DOI":"10.1139\/cjm-2017-0380","type":"journal-article","created":{"date-parts":[[2017,9,18]],"date-time":"2017-09-18T21:37:26Z","timestamp":1505770646000},"page":"929-938","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":41,"title":["MexXY efflux pump overexpression and aminoglycoside resistance in cystic fibrosis isolates of <i>Pseudomonas aeruginosa<\/i> from chronic infections"],"prefix":"10.1139","volume":"63","author":[{"given":"Manu","family":"Singh","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne C.W.","family":"Yau","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Department of Paediatric Laboratory Medicine, The Hospital for Sick Children, Toronto, Ontario, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shirley","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerie","family":"Waters","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Paediatrics, The Hospital for Sick Children, Toronto, Ontario, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayush","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada."},{"name":"Manitoba Chemosensory Biology Group, University of Manitoba, Winnipeg, Manitoba, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"issue":"11","key":"refg1\/ref1","doi-asserted-by":"crossref","first-page":"2624","DOI":"10.1128\/AAC.43.11.2624","volume":"43","author":"Aires J.R.","year":"1999","journal-title":"Antimicrob. 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