{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T02:20:25Z","timestamp":1789698025813,"version":"4.0.1"},"reference-count":38,"publisher":"Canadian Science Publishing","issue":"1","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:p> Serotyping of Salmonella enterica subsp. enterica is a critical step for foodborne salmonellosis investigation. To identify Salmonella enterica subsp. enterica serovars, we have developed a new assay based on a triplex polymerase chain reaction (PCR) with pyrosequencing for amplicon confirmation and phylogenetic discrimination of strains. The top 54 most prevalent serovars of S. enterica in Canada were examined with a total of 23 single-nucleotide polymorphisms (SNPs) and (or) single-nucleotide variations (SNVs) located on 3 genes (fliD, sopE2, and spaO). Seven of the most common serovars, Newport, Typhi, Javiana, Infantis, Thompson, Heidelberg, and Enteritidis, were successfully distinguished from the other serovars based on their unique SNP\u2013SNV combinations. The remaining serovars, including Typhimurium, ssp I:4,[5],12:i:\u2013, and Saintpaul, were further divided into 47 subgroups that demonstrate the relatedness to phylogenetic classifications of each serovar. This pyrosequencing assay is not only cost-effective, rapid, and user-friendly, but also provides phylogenetic information by analyzing 23 selected SNPs. With the added layer of confidence in the PCR results and the accuracy and speed of pyrosequencing, this novel method would benefit the food industry and provides a tool for rapid outbreak investigation through quick detection and identification of common S. enterica serovars in Canada. <\/jats:p>","DOI":"10.1139\/cjm-2017-0496","type":"journal-article","created":{"date-parts":[[2017,10,31]],"date-time":"2017-10-31T21:37:50Z","timestamp":1509485870000},"page":"75-86","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Rapid detection and serovar identification of common <i>Salmonella enterica<\/i> serovars in Canada using a new pyrosequencing assay"],"prefix":"10.1139","volume":"64","author":[{"given":"Maika","family":"Furukawa","sequence":"first","affiliation":[{"name":"Canadian Food Inspection Agency (CFIA) National Centres for Animal Disease, Lethbridge Laboratory, P.O. Box 640, Township Road 9-1, Lethbridge, AB T1J 3Z4, Canada."},{"name":"Laboratory of Veterinary Public Health, Department of Veterinary Medical Science, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki, 889-2192, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriko","family":"Goji","sequence":"additional","affiliation":[{"name":"Canadian Food Inspection Agency (CFIA) National Centres for Animal Disease, Lethbridge Laboratory, P.O. Box 640, Township Road 9-1, Lethbridge, AB T1J 3Z4, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy W.","family":"Janzen","sequence":"additional","affiliation":[{"name":"Canadian Food Inspection Agency (CFIA) National Centres for Animal Disease, Lethbridge Laboratory, P.O. Box 640, Township Road 9-1, Lethbridge, AB T1J 3Z4, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew C.","family":"Thomas","sequence":"additional","affiliation":[{"name":"Canadian Food Inspection Agency (CFIA) National Centres for Animal Disease, Lethbridge Laboratory, P.O. Box 640, Township Road 9-1, Lethbridge, AB T1J 3Z4, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dele","family":"Ogunremi","sequence":"additional","affiliation":[{"name":"Canadian Food Inspection Agency (CFIA) Ontario Laboratory Network, 3851 Fallowfield Road, Ottawa, ON K2H 8P9, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Burton","family":"Blais","sequence":"additional","affiliation":[{"name":"Canadian Food Inspection Agency (CFIA) Ontario Laboratory Network, Building 22, 960 Carling Avenue, Ottawa, ON K1A 0C6, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoaki","family":"Misawa","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Public Health, Department of Veterinary Medical Science, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki, 889-2192, Japan."},{"name":"Center for Animal Disease Control, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki, 889-2192, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kingsley K.","family":"Amoako","sequence":"additional","affiliation":[{"name":"Canadian Food Inspection Agency (CFIA) National Centres for Animal Disease, Lethbridge Laboratory, P.O. Box 640, Township Road 9-1, Lethbridge, AB T1J 3Z4, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002776"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2013.03.003"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0055254"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/781652"},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0055687"},{"key":"refg7\/ref7","doi-asserted-by":"publisher","DOI":"10.1128\/microbe.11.311.1"},{"key":"refg8\/ref8","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-12-425"},{"key":"refg9\/ref9","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-091313-103739"},{"key":"refg10\/ref10","doi-asserted-by":"publisher","DOI":"10.1007\/0-387-30746-X_7"},{"key":"refg11\/ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2014.12.003"},{"key":"refg12\/ref12","unstructured":"Grimont, P.A.D., and Weill, F.X. 2007. Antigenic formulae of the Salmonella serovars. 9th ed. WHO Collaborating Centre for Reference and Research on Salmonella, Institut Pasteur, Paris, France."},{"key":"refg13\/ref13","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00154-16"},{"key":"refg14\/ref14","doi-asserted-by":"publisher","DOI":"10.1038\/ng.195"},{"key":"refg15\/ref15","doi-asserted-by":"publisher","DOI":"10.1128\/jb.169.3.1168-1173.1987"},{"key":"refg16\/ref16","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X.JFP-14-216"},{"key":"refg17\/ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2016.12.005"},{"key":"refg18\/ref18","doi-asserted-by":"publisher","DOI":"10.1126\/science.1201476"},{"key":"refg19\/ref19","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02095-08"},{"key":"refg20\/ref20","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-13-88"},{"key":"refg21\/ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2012.01.020"},{"key":"refg22\/ref22","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01371-09"},{"key":"refg23\/ref23","doi-asserted-by":"publisher","DOI":"10.1086\/650733"},{"key":"refg24\/ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2014.10.004"},{"key":"refg25\/ref25","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-4-31"},{"key":"refg26\/ref26","doi-asserted-by":"publisher","DOI":"10.1111\/jam.12678"},{"key":"refg27\/ref27","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.17.5883-5898.2004"},{"key":"refg28\/ref28","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2013.05.031"},{"key":"refg29\/ref29","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03201-12"},{"key":"refg30\/ref30","doi-asserted-by":"publisher","DOI":"10.1101\/gr.11.1.3"},{"key":"refg31\/ref31","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00056-16"},{"key":"refg32\/ref32","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2015.01.009"},{"key":"refg33\/ref33","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00969-10"},{"issue":"6","key":"refg34\/ref34","doi-asserted-by":"crossref","first-page":"1206","DOI":"10.1046\/j.1365-2958.2000.01933.x","volume":"36","author":"Stender S.","year":"2000","journal-title":"Mol. Microbiol."},{"key":"refg35\/ref35","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evt159"},{"key":"refg36\/ref36","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.05527-11"},{"key":"refg37\/ref37","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12827"},{"key":"refg38\/ref38","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2014.11.024"},{"key":"refg39\/ref39","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0147101"}],"container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdnsciencepub.com\/doi\/full-xml\/10.1139\/cjm-2017-0496","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdnsciencepub.com\/doi\/pdf\/10.1139\/cjm-2017-0496","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T16:53:03Z","timestamp":1751475183000},"score":1,"resource":{"primary":{"URL":"https:\/\/cdnsciencepub.com\/doi\/10.1139\/cjm-2017-0496"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1]]}},"alternative-id":["10.1139\/cjm-2017-0496"],"URL":"https:\/\/doi.org\/10.1139\/cjm-2017-0496","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1]]},"assertion":[{"value":"2017-08-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-10-06","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-10-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-10-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}