{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T08:10:04Z","timestamp":1791360604726,"version":"4.3.1"},"reference-count":26,"publisher":"Canadian Science Publishing","issue":"7","license":[{"start":{"date-parts":[[2018,7,1]],"date-time":"2018-07-01T00:00:00Z","timestamp":1530403200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:p> Although Lactobacillus species are recognized as normal inhabitants of porcine gastric mucosa, the association of these bacteria with health status or gastric ulcer disease has never been considered. We investigated the bacterial load of Lactobacillus isolated from the antrum, corpus, and pars esophagea of stomachs with (n = 13) and without (n = 10) ulcer of the pars esophagea of slaughtered pigs. We also evaluated in vitro antagonistic properties against typical pathogens of strains isolated from stomachs without ulcer. To quantify Lactobacillus, gastric mucosa samples obtained with 5 mm biopsy punches were smeared on MRS agar and colonies were counted after 48 h of incubation under anaerobic conditions. The score of Lactobacillus was significantly greater in the antrum and corpus of stomachs without ulcer (P &lt; 0.001 for both) when compared with stomachs with ulcer. Fingerprint profiles, obtained by repetitive sequence-based PCR using (GTG)<jats:sub>5<\/jats:sub> primers, showed that the isolates were highly diverse. The reduction of Lactobacillus load in porcine stomachs may be a contributing factor for gastric ulcer. Strains isolated from healthy stomachs, which showed a wide spectrum of antagonistic activity against pathogens, may be viewed as an untapped source of bacteria with potential beneficial properties that deserve to be further investigated. <\/jats:p>","DOI":"10.1139\/cjm-2017-0758","type":"journal-article","created":{"date-parts":[[2018,3,19]],"date-time":"2018-03-19T21:34:11Z","timestamp":1521495251000},"page":"493-499","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["Porcine stomachs with and without gastric ulcer differ in <i>Lactobacillus<\/i> load and strain characteristics"],"prefix":"10.1139","volume":"64","author":[{"given":"Luciana Ramos","family":"Almeida","sequence":"first","affiliation":[{"name":"Departamento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia Silva","family":"Costa","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Geral, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9a Maria Amaral","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Geral, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana de Paula","family":"Reis","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Geral, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina Oliveira","family":"Barros","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luige Biciati","family":"Alvim","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Geral, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1lvaro Cantini","family":"Nunes","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Geral, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dulciene Maria Magalh\u00e3es","family":"Queiroz","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em Bacteriologia, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte 30130-100, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gifone Aguiar","family":"Rocha","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em Bacteriologia, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte 30130-100, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques Robert","family":"Nicoli","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00edlvia Beleza","family":"de Moura","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1980.tb05216.x"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1080\/08910600050216246-1"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1985.tb00420.x"},{"issue":"1","key":"refg4\/ref4","doi-asserted-by":"crossref","first-page":"34","DOI":"10.54846\/jshap\/381","volume":"12","author":"Friendship R.M.","year":"2004","journal-title":"J. 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