{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T16:07:03Z","timestamp":1788883623329,"version":"build-2803163510"},"reference-count":28,"publisher":"Canadian Science Publishing","issue":"3","license":[{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:p> A biocontrol bacterium, Pseudomonas chlororaphis O6 promotes plant health through multifaceted mechanisms. In this study, we used P. chlororaphis O6 mutants to examine metabolites with aphicidal activity. Direct application of intact P. chlororaphis cells to the surface of second-instar nymphs of the green peach aphid resulted in no mortality. However, nymphs died when exposed only to the volatiles produced by the P. chlororaphis O6 wild-type strain grown on rich media. Mutants lacking the production of two antibiotics, phenazine and pyrrolnitrin, or the insect toxin FitD retained the aphicidal potential of the wild-type strain. However, the volatiles produced by mutants deficient in the production of hydrogen cyanide (HCN) or defective in the synthesis of the global regulator GacS, which regulates HCN synthesis, showed no aphicidal activity. Direct application of potassium cyanide caused mortality of green peach aphid nymphs. These results indicate that HCN production by a plant probiotic is involved in preventing insect growth. <\/jats:p>","DOI":"10.1139\/cjm-2018-0372","type":"journal-article","created":{"date-parts":[[2018,11,6]],"date-time":"2018-11-06T21:33:59Z","timestamp":1541540039000},"page":"185-190","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":22,"title":["Hydrogen cyanide produced by <i>Pseudomonas chlororaphis<\/i> O6 is a key aphicidal metabolite"],"prefix":"10.1139","volume":"65","author":[{"given":"Beom Ryong","family":"Kang","sequence":"first","affiliation":[{"name":"Department of Applied Biology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, South Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne J.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Department of Biological Engineering, Utah State University, Logan, UT 84322-4105, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Cheol","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Applied Biology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, South Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/18.2.265a"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cropro.2017.11.009"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2011.02582.x"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01968-10"},{"key":"refg5\/ref5","unstructured":"Finney, D.J. 1971. 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