{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T14:44:58Z","timestamp":1780497898152,"version":"3.54.1"},"reference-count":39,"publisher":"Canadian Science Publishing","issue":"7","license":[{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:p> Salmonella enterica serovar Heidelberg causes foodborne infections and is a major threat to the food chain and public health. In this study, we aimed to develop a rapid molecular typing approach to identify Salmonella enterica serovar Heidelberg. Using comparative genomics, four serovar-specific gene fragments were identified, and a real-time polymerase chain reaction (PCR) combined with a propidium monoazide (PMA) pretreatment method was developed for simultaneous detection of viable Salmonella sp. (invA) and Salmonella Heidelberg (SeHA_C3258). The assay showed 100% specificity for all strains tested. The assay was able to distinguish effectively viable or dead cells with the PMA. The detection limit was 2.4 CFU\/mL following 6 h of incubation in enrichment Luria\u2013Bertani medium, and the assay could detect 1.7 \u00d7 10<jats:sup>2<\/jats:sup> CFU\/mL in the presence of pork background flora. In artificially contaminated pork, real-time PCR detected inoculum levels of 1.15 CFU\/25 g of pork after a 6 h enrichment. Thus, our findings indicated that this comparative genomics approach could be used to screen for serovar-specific fragments and that real-time PCR with PMA was a simple and reliable method for detecting viability of Salmonella species and Salmonella Heidelberg. <\/jats:p>","DOI":"10.1139\/cjm-2018-0547","type":"journal-article","created":{"date-parts":[[2019,3,13]],"date-time":"2019-03-13T21:36:30Z","timestamp":1552512990000},"page":"477-485","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":11,"title":["Propidium monoazide real-time PCR amplification for viable <i>Salmonella<\/i> species and <i>Salmonella<\/i> Heidelberg in pork"],"prefix":"10.1139","volume":"65","author":[{"given":"Ligong","family":"Zhai","sequence":"first","affiliation":[{"name":"College of Food Science and Technology, Nanjing Agricultural University, Key Laboratory of Food Processing and Quality Control, Ministry of Agriculture of China, Nanjing, Jiangsu Province, China."},{"name":"College of Food Engineering, Anhui Science and Technology University Chuzhou, Anhui Province, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjie","family":"Li","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Nanjing Agricultural University, Key Laboratory of Food Processing and Quality Control, Ministry of Agriculture of China, Nanjing, Jiangsu Province, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Tao","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Nanjing Agricultural University, Key Laboratory of Food Processing and Quality Control, Ministry of Agriculture of China, Nanjing, Jiangsu Province, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoxin","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Nanjing Agricultural University, Key Laboratory of Food Processing and Quality Control, Ministry of Agriculture of China, Nanjing, Jiangsu Province, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengxia","family":"Lv","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Nanjing Agricultural University, Key Laboratory of Food Processing and Quality Control, Ministry of Agriculture of China, Nanjing, Jiangsu Province, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomei","family":"Bie","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Nanjing Agricultural University, Key Laboratory of Food Processing and Quality Control, Ministry of Agriculture of China, Nanjing, Jiangsu Province, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-89"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.4103\/2276-7096.192237"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.592-600.2003"},{"key":"refg5\/ref5","doi-asserted-by":"publisher","DOI":"10.1637\/7283-092404R1.1"},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2009.12.004"},{"key":"refg7\/ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2014.12.011"},{"key":"refg8\/ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2011.04.015"},{"key":"refg9\/ref9","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02686-08"},{"key":"refg10\/ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2012.10.003"},{"key":"refg11\/ref11","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-016-7039-9"},{"key":"refg12\/ref12","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00004-12"},{"key":"refg13\/ref13","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-178"},{"key":"refg14\/ref14","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(99)00102-6"},{"key":"refg15\/ref15","doi-asserted-by":"publisher","DOI":"10.3201\/eid1908.121511"},{"key":"refg16\/ref16","doi-asserted-by":"publisher","DOI":"10.1086\/381580"},{"key":"refg17\/ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2015.09.014"},{"key":"refg18\/ref18","doi-asserted-by":"publisher","DOI":"10.1111\/jam.12630"},{"key":"refg19\/ref19","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00701-06"},{"key":"refg20\/ref20","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X.JFP-12-126"},{"key":"refg21\/ref21","doi-asserted-by":"publisher","DOI":"10.1016\/S0895-3988(08)60019-7"},{"issue":"6","key":"refg22\/ref22","doi-asserted-by":"crossref","first-page":"1541","DOI":"10.1128\/aem.56.6.1541-1546.1990","volume":"56","author":"Lee H.A.","year":"1990","journal-title":"Appl. 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