{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T23:31:50Z","timestamp":1762299110364,"version":"3.41.0"},"reference-count":39,"publisher":"Canadian Science Publishing","issue":"1","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p> The use of the food-grade bacterium Lactococcus lactis as a new cell factory is a promising alternative expression system for producing a desired protein. The Omp16-IL2 fusion protein antigen was cloned, expressed, and purified in this study. The Omp16-IL2 fusion gene was designed and cloned in pGH plasmid with appropriate restriction sites and subcloned in pAMJ2008 expression vector digested with the same enzymes. The purified recombinant constructed pAMJ-rOmp-IL2 was introduced into L. lactis subsp. cremoris MG1363 by electrotransformation. Finally, the expression and purification of Omp16-IL2 fusion protein was investigated. This study reports the construction of a recombinant L. lactis expressing the Omp16-IL2 fusion protein as an oral Lactococcus-based vaccine, as compared with commonly used live attenuated vaccines, for future studies against brucellosis. <\/jats:p>","DOI":"10.1139\/cjm-2019-0261","type":"journal-article","created":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T21:34:03Z","timestamp":1569965643000},"page":"39-45","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":12,"title":["Production of <i>Brucella melitensis<\/i> Omp16 protein fused to the human interleukin 2 in <i>Lactococcus lactis<\/i> MG1363 toward developing a <i>Lactococcus<\/i>-based vaccine against brucellosis"],"prefix":"10.1139","volume":"66","author":[{"given":"Marzieh","family":"Rezaei","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Islamic Republic of Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad","family":"Rabbani Khorasgani","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Islamic Republic of Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sayyed Hamid","family":"Zarkesh Esfahani","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Islamic Republic of Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rahman","family":"Emamzadeh","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Islamic Republic of Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hamid","family":"Abtahi","sequence":"additional","affiliation":[{"name":"Molecular and Medicine Research Center, Arak University of Medical Science, Arak, Islamic Republic of Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02297320"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2013.06.002"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-47-1-39"},{"key":"refg5\/ref5","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00472-06"},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00211-0"},{"key":"refg7\/ref7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2006.00145.x"},{"key":"refg8\/ref8","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-015-0199-7"},{"key":"refg9\/ref9","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnw234"},{"key":"refg10\/ref10","first-page":"192","volume":"12","author":"Ghasemi A.","year":"2013","journal-title":"Iran J. Allergy Asthma Immunol."},{"key":"refg11\/ref11","doi-asserted-by":"publisher","DOI":"10.1016\/0958-1669(93)90056-3"},{"key":"refg12\/ref12","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-48-9-833"},{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.6b00424"},{"key":"refg13\/ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.04.036"},{"key":"refg14\/ref14","doi-asserted-by":"publisher","DOI":"10.5455\/pmb.1-1309507875"},{"key":"refg15\/ref15","doi-asserted-by":"publisher","DOI":"10.1385\/0-89603-310-4:195"},{"key":"refg16\/ref16","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6968.12351"},{"key":"refg17\/ref17","doi-asserted-by":"publisher","DOI":"10.1016\/s0005-2736(02)00712-5"},{"key":"refg18\/ref18","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"refg19\/ref19","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-4-2"},{"key":"refg20\/ref20","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01326.x"},{"key":"refg21\/ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2004.03.007"},{"key":"refg22\/ref22","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-5-14"},{"key":"refg23\/ref23","doi-asserted-by":"publisher","DOI":"10.1110\/ps.051689905"},{"key":"refg24\/ref24","doi-asserted-by":"publisher","DOI":"10.1159\/000106082"},{"key":"refg25\/ref25","doi-asserted-by":"publisher","DOI":"10.1517\/13543784.15.10.1141"},{"key":"refg26\/ref26","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2010.00211.x"},{"key":"refg27\/ref27","doi-asserted-by":"publisher","DOI":"10.1080\/10611860410001670035"},{"key":"refg28\/ref28","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26299-0"},{"key":"refg29\/ref29","doi-asserted-by":"publisher","DOI":"10.2174\/1871526518666180709121653"},{"key":"refg30\/ref30","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.2.910-916.2002"},{"key":"refg31\/ref31","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.12.088"},{"key":"refg32\/ref32","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00676.x"},{"key":"refg33\/ref33","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00638-08"},{"issue":"7","key":"refg34\/ref34","doi-asserted-by":"crossref","first-page":"3183","DOI":"10.1128\/IAI.66.7.3183-3189.1998","volume":"66","author":"Steidler L.","year":"1998","journal-title":"Infect. Immun."},{"key":"refg35\/ref35","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5483.1352"},{"issue":"1","key":"refg36\/ref36","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1128\/iai.64.1.100-107.1996","volume":"64","author":"Tibor A.","year":"1996","journal-title":"Infect. Immun."},{"issue":"9","key":"refg37\/ref37","doi-asserted-by":"crossref","first-page":"4960","DOI":"10.1128\/IAI.67.9.4960-4962.1999","volume":"67","author":"Tibor A.","year":"1999","journal-title":"Infect. Immun."},{"key":"refg38\/ref38","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2008.02029.x"},{"key":"refg39\/ref39","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-015-6498-0"}],"container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdnsciencepub.com\/doi\/full-xml\/10.1139\/cjm-2019-0261","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdnsciencepub.com\/doi\/pdf\/10.1139\/cjm-2019-0261","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T17:50:30Z","timestamp":1751478630000},"score":1,"resource":{"primary":{"URL":"https:\/\/cdnsciencepub.com\/doi\/10.1139\/cjm-2019-0261"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":39,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1139\/cjm-2019-0261"],"URL":"https:\/\/doi.org\/10.1139\/cjm-2019-0261","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"type":"print","value":"0008-4166"},{"type":"electronic","value":"1480-3275"}],"subject":[],"published":{"date-parts":[[2020,1]]},"assertion":[{"value":"2019-05-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-05","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}