{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T13:53:39Z","timestamp":1781013219397,"version":"3.54.1"},"reference-count":33,"publisher":"Canadian Science Publishing","issue":"5","license":[{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:p> Iron is a fundamental element required by most organisms, including Brucella. Several researchers have suggested that the iron response regulator (irr) and rhizobial iron regulator (rirA) genes regulate iron acquisition by Brucella abortus, influencing heme synthesis by and virulence of this pathogen. However, little is known about another Brucella species, Brucella melitensis. In this research, we successfully constructed two mutants: M5-90\u0394irr and M5-90\u0394rirA. The adhesion, invasion, and intracellular survivability of these two mutants were evaluated in RAW264.7 cells infected with 1 \u00d7 10<jats:sup>6<\/jats:sup> CFU of M5-90\u0394irr, M5-90\u0394rirA, or M5-90. We also tested the sensitivity of cells to hydrogen peroxide and their ability to grow. In addition, the virulence of these two mutants was evaluated in BALB\/c mice. The results showed that the ability of these two mutants to invade and adhere inside the murine macrophages RAW264.7 was attenuated but their ability to replicate intracellularly was strengthened, enhancing the resistance to hydrogen peroxide. The M5-90\u0394irr mutant showed stronger growth ability than the parental strain under iron-limiting conditions. No differences were observed in the number of bacteria in spleen between M5-90 and M5-90\u0394irr at 7 or 15 days postinfection. However, the number of M5-90\u0394rirA in spleen reduced significantly at 15 days postinfection. The splenic index of the M5-90\u0394irr group is evidently lower than that of M5-90. This is the first report that irr and rirA genes of B. melitensis are associated not only with virulence but also with growth ability. Together, our data suggest that M5-90\u0394irr is a promising Brucella vaccine candidate. <\/jats:p>","DOI":"10.1139\/cjm-2019-0393","type":"journal-article","created":{"date-parts":[[2020,2,10]],"date-time":"2020-02-10T21:38:10Z","timestamp":1581370690000},"page":"351-358","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":11,"title":["Insights into <i>irr<\/i> and <i>rirA<\/i> gene regulation on the virulence of <i>Brucella melitensis<\/i> M5-90"],"prefix":"10.1139","volume":"66","author":[{"given":"Huan","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Animal Science and Technology, Shihezi University, Shihezi City, Xinjiang 832000, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benben","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Science, Shihezi University, Shihezi City, Xinjiang 832000, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxing","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology, Shihezi University, Shihezi City, Xinjiang 832000, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Deng","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology, Shihezi University, Shihezi City, Xinjiang 832000, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiran","family":"Shao","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology, Shihezi University, Shihezi City, Xinjiang 832000, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jihai","family":"Yi","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology, Shihezi University, Shihezi City, Xinjiang 832000, P.R. 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