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Bacterial communities within the drinking water were dominated by Escherichia, whereas communities in litter were more representative of the cecum. The crop and small intestine (jejunum and ileum) were dominated by Lactobacillus and Enterococcus spp., and the cecum was dominated by Proteus spp. The collection of bacteria isolated was dominated by Enterococcus spp., Escherichia\/Shigella spp., Lactobacillus spp., and Proteus spp.; however, many rare taxa were observed. These included members of the Clostridiales and Clostridium spp., which were commonly isolated from the ileum and cecum. Bacteria isolated by enrichment and direct plating differed. The selective de Man\u2013Rogosa\u2013Sharpe agar was commonly associated with the isolation of Lactobacillus spp. and yielded the lowest diversity of all methods utilized. Increased diversity and frequency of Clostridium spp. was observed in enrichments of blood and mucus or by plating on Columbia agar supplemented with 10% blood and gentamicin. The bacteria isolated from this study provide source material for genomic and functional studies in chicken hosts. <\/jats:p>","DOI":"10.1139\/cjm-2019-0469","type":"journal-article","created":{"date-parts":[[2020,1,27]],"date-time":"2020-01-27T21:35:59Z","timestamp":1580160959000},"page":"288-302","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":11,"title":["Application of culturomics to characterize diverse anaerobic bacteria from the gastrointestinal tract of broiler chickens in relation to environmental reservoirs"],"prefix":"10.1139","volume":"66","author":[{"given":"Paul E.","family":"Moote","sequence":"first","affiliation":[{"name":"Agriculture and Agri-Food Canada, 5403-1st Avenue South, Lethbridge, AB T1J 4B1, Canada."},{"name":"Department of Agricultural, Food and Nutritional Science, 410 Agriculture\/Forestry Centre, University of Alberta, Edmonton, AB T6G 2P5, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah J.M.","family":"Zaytsoff","sequence":"additional","affiliation":[{"name":"Agriculture and Agri-Food Canada, 5403-1st Avenue South, Lethbridge, AB T1J 4B1, Canada."},{"name":"Department of Agricultural, Food and Nutritional Science, 410 Agriculture\/Forestry Centre, University of Alberta, Edmonton, AB T6G 2P5, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo","family":"Ortega Polo","sequence":"additional","affiliation":[{"name":"Agriculture and Agri-Food Canada, 5403-1st Avenue South, Lethbridge, AB T1J 4B1, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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