{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T14:29:26Z","timestamp":1781620166896,"version":"3.54.5"},"reference-count":39,"publisher":"Canadian Science Publishing","issue":"3","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:p> Brucella abortus is a gram-negative intracellular parasite bacteria that causes serious health hazards in humans and animals. The type IV secretion system (T4SS), encoded by the virB promoter, has been identified as an important virulence factor for Brucella abortus, but its impact on Brucella abortus A19 remains unclear. In this study, the T4SS of Brucella abortus A19 was inactivated by deletion of the virB promoter, resulting in a mutant strain A19\u0394virB. Real-time PCR and western blotting analysis demonstrated that T4SS-related proteins were not expressed after virB promoter deletion. Moreover, the survival rate of A19 in high-salt and strong acidic environments decreased after virB promoter deletion. Compared to the parental strain A19, the A19\u0394virB mutant strain showed reduced growth rate in TSB, decreased invasion ability to macrophages and dendritic cells, and reduced virulence of the mutant strain in macrophages, dendritic cells, and mice. In addition, the A19\u0394virB mutant strain showed enhanced autophagy in macrophages and dendritic cells compared with A19, and the A19\u0394virB mutant strain was able to upregulate IL-6 and downregulate IL-10 in macrophages. These data help us to better understand the T4SS of the A19 vaccine strain and contribute to our efforts to improve Brucella vaccines. <\/jats:p>","DOI":"10.1139\/cjm-2021-0053","type":"journal-article","created":{"date-parts":[[2021,10,13]],"date-time":"2021-10-13T21:35:36Z","timestamp":1634160936000},"page":"165-176","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["Deletion of the type IV secretion system promoter <i>VirB<\/i> in <i>Brucella abortus<\/i> A19 strain attenuated the virulence of the bacteria and promotes autophagy"],"prefix":"10.1139","volume":"68","author":[{"given":"Xiaoyu","family":"Deng","sequence":"first","affiliation":[{"name":"School of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinke","family":"He","sequence":"additional","affiliation":[{"name":"Department of Pathology, Institutes of Basic Medicine, Xinjiang Second Medical College, Kelamayi, Xinjiang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueli","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jihai","family":"Yi","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhe","family":"Miao","sequence":"additional","affiliation":[{"name":"Sunnersw Biotechnology Co., Ltd., Nan Ping, Fu Jian, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuangfu","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201040497"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.13685"},{"issue":"6","key":"refg3\/ref3","first-page":"2379","volume":"31","author":"Basyony A.F.","year":"2018","journal-title":"Pak. 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