{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T22:51:37Z","timestamp":1788907897653,"version":"build-2803163510"},"reference-count":39,"publisher":"Canadian Science Publishing","issue":"2","license":[{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:p> While monitoring the presence of antibiotic resistance in municipal wastewater bacteria from Mexico City, five Escherichia coli isolates were found to be resistant to carbapenems, antibiotics of \u201clast resort\u201d used mostly in hospitals. Further analysis revealed that these carbapenem-resistant isolates carried the gene encoding a metallo-beta-lactamase, NDM-5. The gene was found to be beared by a large, \u223c145 kb conjugative plasmid, which also carries putative genes encoding resistance to sulfonamides, trimethoprim, tetracycline, ciprofloxacin, and chloramphenicol (although no phenotypic chloramphenicol resistance was detected) and quaternary-ammonium compounds. The plasmid also carried gene mobility determinants, such as integron integrase and two transposases. In addition to the direct public health threat posed by the presence of such multi-resistant organisms in wastewater released into the environment and used for crop irrigation; it is particularly concerning that carbapenem-resistant E.\u00a0coli is rather rare in Mexican hospitals (&lt;1%), but was found in small, 100 mL samples of municipal wastewater. This suggests that these organisms are under-reported by clinical microbiology laboratories, underlining the usefulness of wastewater monitoring, or that there is an unknown source of such carbapenem-resistant organisms that are being dumped into the wastewater. The source of these bacteria must be assessed and controlled to prevent further spread of this multi-resistance plasmid among other environmental and clinical microorganisms. <\/jats:p>","DOI":"10.1139\/cjm-2021-0284","type":"journal-article","created":{"date-parts":[[2021,10,18]],"date-time":"2021-10-18T22:14:59Z","timestamp":1634595299000},"page":"139-145","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Metallo-beta-lactamase-producing <i>Escherichia coli<\/i> in the sewage of Mexico City: where do they come from?"],"prefix":"10.1139","volume":"68","author":[{"given":"Kathia","family":"L\u00fcneberg","sequence":"first","affiliation":[{"name":"Laboratorio de Aerobiolog\u00eda, Centro de Ciencias de la Atm\u00f3sfera, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos F.","family":"Am\u00e1bile-Cuevas","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Lusara, Mexico City, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Mucito-Varela","sequence":"additional","affiliation":[{"name":"Programa de Inmunolog\u00eda Molecular Microbiana, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leticia","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Laboratorio de Aerobiolog\u00eda, Centro de Ciencias de la Atm\u00f3sfera, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Salinas","sequence":"additional","affiliation":[{"name":"Laboratorio de Aerobiolog\u00eda, Centro de Ciencias de la Atm\u00f3sfera, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yolanda","family":"L\u00f3pez-Vidal","sequence":"additional","affiliation":[{"name":"Programa de Inmunolog\u00eda Molecular Microbiana, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Siebe","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias Ambientales y del Suelo, Instituto de Geolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irma","family":"Rosas","sequence":"additional","affiliation":[{"name":"Laboratorio de Aerobiolog\u00eda, Centro de Ciencias de la Atm\u00f3sfera, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2012.0115"},{"key":"refg2\/ref2","unstructured":"Am\u00e1bile-Cuevas, C.F. 2016. 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