{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T10:43:30Z","timestamp":1763117010222,"version":"3.41.0"},"reference-count":60,"publisher":"Canadian Science Publishing","issue":"8","license":[{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/deed.en_GB"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2023,8,1]]},"abstract":"<jats:p> Hamilton Harbour is an impaired embayment of Lake Ontario that experiences seasonal algal blooms despite decades of remedial efforts. To study the harbour\u2019s cyanobacterial and heterotrophic bacterial communities, we extracted and sequenced community DNA from surface water samples collected biweekly from different sites during summer and fall. Assembled contigs were annotated at the phylum level, and Cyanobacteria were further characterized at order and species levels. Actinobacteria were most abundant in early summer, while Cyanobacteria were dominant in mid-summer. Microcystis aeruginosa and Limnoraphis robusta were most abundant throughout the sampling period, expanding the documented diversity of Cyanobacteria in Hamilton Harbour. Functional annotations were performed using the MG-RAST pipeline and SEED database, revealing that genes for photosynthesis, nitrogen metabolism, and aromatic compound metabolism varied in relative abundances over the season, while phosphorus metabolism was consistent, suggesting that these genes remained essential despite fluctuating environmental conditions and community succession. We observed seasonal shifts from anoxygenic to oxygenic phototrophy, and from ammonia assimilation to nitrogen fixation, coupled with decreasing heterotrophic bacteria and increasing Cyanobacteria relative abundances. Our data contribute important insights into bacterial taxa and functional potentials in Hamilton Harbour, revealing seasonal and spatial dynamics that can be used to inform ongoing remediation efforts. <\/jats:p>","DOI":"10.1139\/cjm-2022-0252","type":"journal-article","created":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T21:40:00Z","timestamp":1681422000000},"page":"281-295","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Analysis of bacterioplankton genes in an impaired Great Lakes harbour reveals seasonal metabolic shifts and a previously undetected cyanobacterium"],"prefix":"10.1139","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3037-0064","authenticated-orcid":false,"given":"Christine N.","family":"Palermo","sequence":"first","affiliation":[{"name":"Department of Physical and Environmental Sciences, University of Toronto Mississauga","place":["Mississauga, Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberta R.","family":"Fulthorpe","sequence":"additional","affiliation":[{"name":"Department of Physical and Environmental Sciences, University of Toronto Scarborough","place":["Scarborough, Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosemary","family":"Saati","sequence":"additional","affiliation":[{"name":"Department of Physical and Environmental Sciences, University of Toronto Scarborough","place":["Scarborough, Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6576-4883","authenticated-orcid":false,"given":"Steven M.","family":"Short","sequence":"additional","affiliation":[{"name":"Department of Physical and Environmental Sciences, University of Toronto Mississauga","place":["Mississauga, Canada"]},{"name":"Department of Biology, University of Toronto Mississauga","place":["Mississauga, Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00923"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0106588"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061011"},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3176"},{"key":"refg7\/ref7","doi-asserted-by":"publisher","DOI":"10.1186\/s40793-020-00365-8"},{"issue":"22","key":"refg9\/ref9","doi-asserted-by":"crossref","first-page":"12 830","DOI":"10.1073\/pnas.2133554100","volume":"100","author":"de la Torre J.R.","year":"2003","journal-title":"Proc. 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