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Microbiol."],"published-print":{"date-parts":[[2024,1,1]]},"abstract":"<jats:p>Ribonucleoprotein granules are bio-condensates that form a diverse group of dynamic membrane-less organelles implicated in several cellular functions, including stress response and cellular survival. In Toxoplasma gondii, a type of bio-condensates referred to as stress granules (SGs) are formed prior to the parasites\u2019 egress from the host cell and are implicated in the survival and invasion competency of extracellular tachyzoites. We used paraformaldehyde to fix and cross-link SG proteins to allow purification by centrifugation and analysis by mass spectrometry. We profiled protein components of SGs at 10 and 30\u00a0min post-egress when parasite\u2019s invasion ability is significantly diminished. Thirty-three proteins were identified from 10\u00a0min SGs, and additional 43 proteins were identified from 30\u00a0min SGs. Notably, common SG components such as proteins with intrinsically disordered domains were not identified. Gene ontology analysis of both 10 and 30\u00a0min SGs shows that overall molecular functions of SGs\u2019 proteins are ATP-binding, GTP-binding, and GTPase activity. Discernable differences between 10 and 30\u00a0min SGs are in the proportions of translation and microtubule-related proteins. Ten-minute SGs have a higher proportion of microtubule-related proteins and a lower proportion of ribosome-related proteins, while a reverse correlation was identified for those of 30\u00a0min. It remains to be investigated whether this reverse correlation contributes to the ability of extracellular tachyzoites to reinvade host cells.<\/jats:p>","DOI":"10.1139\/cjm-2023-0091","type":"journal-article","created":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T17:34:59Z","timestamp":1697132099000},"page":"32-39","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Proteomic profile of\n                    <i>Toxoplasma<\/i>\n                    <i>gondii<\/i>\n                    stress granules by high-resolution mass spectrometry"],"prefix":"10.1139","volume":"70","author":[{"given":"Scott","family":"Roscoe","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Windsor","place":["Windsor, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Chemistry and 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