{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T10:58:26Z","timestamp":1787223506997,"version":"build-2736575974"},"reference-count":46,"publisher":"Canadian Science Publishing","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/deed.en_GB"}],"funder":[{"name":"Government of Canada Shared Priority Project, Genomics Research and Development Initiative","award":["Project"],"award-info":[{"award-number":["Project"]}]},{"name":"Government of Canada Shared Priority Project, Genomics Research and Development Initiative","award":["GRDI-AMR-One-Health"],"award-info":[{"award-number":["GRDI-AMR-One-Health"]}]}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2025,1,1]]},"abstract":"<jats:p>Ingestion of antibiotic-resistant bacteria following antibiotic treatments may lead to the transfer of antimicrobial resistance genes (ARGs) within a disturbed gut microbiota. However, it remains unclear whether and how microbes present in food matrices influence ARG transfer. Thus, a previously established mouse model, which demonstrated the conjugative transfer of a multi-drug resistance plasmid (pIncA\/C) from Salmonella Heidelberg (donor) to Salmonella Typhimurium (recipient), was used to assess the effects of food-borne microbes derived from fresh carrots on pIncA\/C transfer. Mice were pre-treated with ampicillin, streptomycin, sulfamethazine, or left untreated as a control to facilitate bacterial colonization. Contrary to previous findings where high-density colonization of the donor and recipient bacteria occurred in the absence of food-borne microbes, the presence of these microbes resulted in a low abundance of S. Typhimurium and no detection of S. Typhimurium transconjugants in the fecal samples from any of the mice. However, in mice pre-treated with streptomycin, a significant reduction in microbial species richness allowed for the significant enrichment of Enterobacteriaceae and pIncA\/C transfer to bacteria from the genera Escherichia, Enterobacter, Citrobacter, and Proteus. These findings suggest that food-borne microbes may enhance ARG dissemination by influencing the population dynamics of bacterial hosts within a pre-disturbed gut microbiome.<\/jats:p>","DOI":"10.1139\/cjm-2024-0168","type":"journal-article","created":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T17:34:14Z","timestamp":1746207254000},"page":"1-11","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Food-borne microbes influence conjugative transfer of antimicrobial resistance plasmids in pre-disturbed gut microbiome"],"prefix":"10.1139","volume":"71","author":[{"given":"Marie","family":"Ottenbrite","sequence":"first","affiliation":[{"name":"Ottawa Laboratory-Fallowfield, Canadian Food Inspection Agency","place":["Ottawa, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gokhan","family":"Yilmaz","sequence":"additional","affiliation":[{"name":"Ottawa Laboratory-Fallowfield, Canadian Food 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