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J. Microbiol."],"published-print":{"date-parts":[[2025,1,1]]},"abstract":"<jats:p> Antimicrobial resistance (AMR) is a global public health threat, but the role of foods in its dissemination is poorly understood. We examined the incidence of foodborne bacteria carrying AMR genes considered high-priority research targets by the World Health Organization. Frozen, ready-to-eat, avocado, coconut, mango, and peach ( n\u00a0=\u00a0161) were tested for bacteria encoding extended-spectrum \u03b2-lactamases (ESBLs) and carbapenemases. Over 600 presumptive-positive isolates were recovered and analyzed with a pooled sequencing (Pool-seq) strategy. Coconut samples exhibited the highest bacterial loads and prevalence\/diversity of AMR genes. Isolates harbouring the \u03b2-lactamase genes bla<jats:sub>ctx-m<\/jats:sub>, bla<jats:sub>tem<\/jats:sub>, and bla<jats:sub>shv<\/jats:sub>, identified in 14 coconut and 2 mango samples, were further characterized by whole-genome sequencing and antimicrobial susceptibility testing. The most common gene was bla<jats:sub>ctx-m-15<\/jats:sub>, detected in 20 unique strains. Two carbapenemase-producing strains were isolated from coconut: Enterobacter roggenkampii encoding bla<jats:sub>ndm-1<\/jats:sub> and Escherichia coli encoding bla<jats:sub>ndm-5<\/jats:sub>. Subsequent quantitative PCR (qPCR) analysis of enrichments for bla<jats:sub>ctx-m<\/jats:sub>\/ bla<jats:sub>ndm<\/jats:sub> indicated a potentially higher prevalence of these genes than observed by colony screening. This study presents a practical method for recovering ESBL- and carbapenemase-producing bacteria from foods. Mapping their distribution in food products is crucial to assessing the role of foods in the global spread of AMR and developing effective public health interventions. <\/jats:p>","DOI":"10.1139\/cjm-2024-0210","type":"journal-article","created":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T21:37:01Z","timestamp":1740519421000},"page":"1-18","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Development and evaluation of a sensitive approach for detection and recovery of third-generation cephalosporin- and carbapenem-resistant Enterobacterales from ready-to-eat frozen stone fruit"],"prefix":"10.1139","volume":"71","author":[{"given":"Adeeb","family":"Nasta","sequence":"first","affiliation":[{"name":"Research and Development","place":["Ottawa, Canada"]},{"name":"Ottawa Laboratory (Carling)","place":["Ottawa, Canada"]},{"name":"Canadian Food Inspection Agency","place":["Ottawa, Canada"]},{"name":"Department of 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