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J. Microbiol."],"published-print":{"date-parts":[[2026,1,1]]},"abstract":"<jats:p>Blue light is a critical signal that triggers fruiting body initiation in mushroom-forming fungi. Pleurotus ostreatus is a cultivated model mushroom. The molecular mechanisms linking light perception to primordia formation remain poorly understood. We functionally characterized the blue light receptor gene PoWC-1, a homolog of Neurospora crassa white collar 1, during morphogenesis. Antisense-mediated silencing of PoWC-1 caused a delay in primordia formation under light\/dark cycles and complete arrest at the undifferentiated mycelial stage under continuous darkness, underscoring its role in developmental progression. In darkness, PoWC-1 silencing downregulated key developmental transcription factors, hom1, hom2, Pofst3, and fst4, suggesting that PoWC-1 may contribute to developmental competence independently of light. Comparative transcriptomics revealed PoWC-1 influences a range of metabolic pathways\u2014such as glycolysis\/gluconeogenesis, the pentose phosphate pathway, and amino sugar metabolism\u2014that may support the energetic and biosynthetic demands of fruiting initiation. In addition, PoWC-1 appears to affect upstream signaling components, including the MAPK pathway and the two-component system, along with a suite of downstream transcription factors. These findings suggest that PoWC-1 functions as a key regulator integrating blue light signals into a multi-layered network involved in metabolic reprogramming, signal transduction, and transcriptional regulation during primordia formation in P. ostreatus. Collectively, this study provides a molecular framework for understanding photomorphogenesis in edible mushrooms.<\/jats:p>","DOI":"10.1139\/cjm-2026-0052","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T21:37:43Z","timestamp":1788212263000},"page":"1-13","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["The blue light receptor gene\n                    <i>PoWC-1<\/i>\n                    plays a role in the formation of\n                    <i>Pleurotus ostreatus<\/i>\n                    primordia"],"prefix":"10.1139","volume":"72","author":[{"given":"Xiaojuan","family":"Hou","sequence":"first","affiliation":[{"name":"Henan Agricultural University","place":["Zhengzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenfeng","family":"Xie","sequence":"additional","affiliation":[{"name":"Henan Agricultural University","place":["Zhengzhou, 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