{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T01:20:12Z","timestamp":1790040012163,"version":"4.0.1"},"reference-count":38,"publisher":"Canadian Science Publishing","issue":"9","license":[{"start":{"date-parts":[[2014,9,1]],"date-time":"2014-09-01T00:00:00Z","timestamp":1409529600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:p> Recent studies have focussed on the association between elevated homocysteine levels with megaloblastic changes and thromboembolic events, but the relationship between occult megaloblastosis (with normal haemoglobin levels) and ischaemic stroke has not been widely explored. The objective of this study is to establish a simple and economical marker for the detection of occult megaloblastosis at the community health care level in developing countries. A hundred patients who met the inclusion criteria were studied. At the 5% level of significance, the levels of cobalamin and folate were significantly lower, while the number of hypersegmented neutrophils on the peripheral smear was higher in patients from Group A (70 patients with high homocysteine) compared with the patients in Group B (30 patients with normal homocysteine). Forty-five (64.2%) of the 70 patients in Group A showed hypersegmentation of neutrophils in the peripheral smear. The high cost and difficulty in performing the vitamin assays limit their use as early markers of megaloblastosis. Hence, we conclude that in developing countries, the detection of hypersegmented neutrophils can be used at the primary healthcare level for early diagnosis of occult megaloblastosis, so that early therapeutic interventions with vitamins can prevent attacks of hyperhomocysteinemia-induced ischaemic stroke. <\/jats:p>","DOI":"10.1139\/cjpp-2014-0096","type":"journal-article","created":{"date-parts":[[2014,6,27]],"date-time":"2014-06-27T21:33:47Z","timestamp":1403904827000},"page":"713-716","source":"Crossref","is-referenced-by-count":2,"title":["Early markers of occult megaloblastosis for low-cost detection of hyperhomocysteinemia in patients with ischaemic stroke: preventive approach for primary health care"],"prefix":"10.1139","volume":"92","author":[{"given":"Sohini Sengupta","family":"Neogi","sequence":"first","affiliation":[{"name":"Department of Laboratory Diagnostic Services and Department of Neurology, St. Stephen\u2019s Hospital, St. Stephen\u2019s Hospital Marg, Tis Hazari, Delhi 110054, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Laboratory Diagnostic Services and Department of Neurology, St. Stephen\u2019s Hospital, St. Stephen\u2019s Hospital Marg, Tis Hazari, Delhi 110054, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Archna","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Laboratory Diagnostic Services and Department of Neurology, St. Stephen\u2019s Hospital, St. Stephen\u2019s Hospital Marg, Tis Hazari, Delhi 110054, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaishree","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Laboratory Diagnostic Services and Department of Neurology, St. Stephen\u2019s Hospital, St. Stephen\u2019s Hospital Marg, Tis Hazari, Delhi 110054, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uma","family":"Khanduri","sequence":"additional","affiliation":[{"name":"Department of Laboratory Diagnostic Services and Department of Neurology, St. Stephen\u2019s Hospital, St. Stephen\u2019s Hospital Marg, Tis Hazari, Delhi 110054, India."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.1096\/fasebj.7.14.7901104","volume":"7","author":"Allen R.H.","year":"1993","journal-title":"FASEB J."},{"key":"refg2\/ref2","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1093\/ajcn\/74.2.157","volume":"74","author":"Antony A.C.","year":"2001","journal-title":"Am. J. Clin. Nutr."},{"key":"refg3\/ref3","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1093\/ajcn\/78.1.3","volume":"78","author":"Antony A.C.","year":"2003","journal-title":"Am. J. Clin. Nutr."},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1159\/000096149"},{"issue":"6","key":"refg5\/ref5","first-page":"414","volume":"49","author":"Bhargava S.","year":"2012","journal-title":"Indian J. Biochem. Biophys."},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.3164\/jcbn.11-72"},{"key":"refg7\/ref7","doi-asserted-by":"publisher","DOI":"10.1001\/jama.274.13.1049"},{"key":"refg8\/ref8","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2003.1.62"},{"key":"refg9\/ref9","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.10093"},{"key":"refg10\/ref10","first-page":"675","volume":"56","author":"Dalal P.M.","year":"2008","journal-title":"J. Assoc. Physicians India"},{"key":"refg11\/ref11","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199603213341203"},{"key":"refg14\/ref14","doi-asserted-by":"crossref","first-page":"S52","DOI":"10.1177\/15648265080292S108","volume":"29","author":"Green R.","year":"2008","journal-title":"Food Nutr. Bull."},{"key":"refg15\/ref15","doi-asserted-by":"publisher","DOI":"10.1016\/S0950-3536(05)80220-3"},{"key":"refg16\/ref16","unstructured":"Green, R. 2010. Folate, cobalamin, and megaloblastic anemias. In Williams hematology. 8th ed. Edited by K. Kaushansky, E. Beutler, U. Seligsohn, M.A. Lichtman, T.J. Kipps, and J.T. Prchal. McGraw-Hill, New York, N.Y. pp. 533\u2013563."},{"key":"refg17\/ref17","unstructured":"Hoffmann, R., Benz, E.J., Shattil, S.J., Furie, B., Cohen, H.J., Silberstein, L.E., and McGlave, P. (Editors). 2005. Hematology: Basic Principles and Practice. 4th ed. Elsevier. pp. 528\u2013529."},{"key":"refg18\/ref18","doi-asserted-by":"publisher","DOI":"10.1001\/jama.288.16.2015"},{"issue":"4","key":"refg19\/ref19","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1093\/ajcn\/58.4.468","volume":"58","author":"Joosten E.","year":"1993","journal-title":"Am. J. Clin. Nutr."},{"issue":"4","key":"refg21\/ref21","first-page":"172","volume":"20","author":"Khanduri U.","year":"2007","journal-title":"Natl. Med. J. India"},{"issue":"4","key":"refg20\/ref20","first-page":"182","volume":"18","author":"Khanduri U.","year":"2005","journal-title":"Natl. Med. J. India"},{"key":"refg22\/ref22","unstructured":"Lee, G.R. 1993. Megaloblastic and nonmegaloblastic changes in macrocytic anaemias. In Clinical Hematology. Edited by G.R. Lee. Lea & Fabiger, Philadelphia, Pa. Vol. 1, pp. 745\u2013790."},{"key":"refg23\/ref23","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1980.tb05922.x"},{"key":"refg24\/ref24","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2362.1999.00446.x"},{"key":"refg25\/ref25","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1111\/j.1532-5415.1996.tb01407.x","volume":"44","author":"Metz J.","year":"1996","journal-title":"J. Am. Geriatr. Soc."},{"key":"refg26\/ref26","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2007.356"},{"key":"refg27\/ref27","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1093\/ajcn\/74.2.233","volume":"74","author":"Refsum H.","year":"2001","journal-title":"Am. J. Clin. Nutr."},{"key":"refg28\/ref28","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199502023320502"},{"key":"refg29\/ref29","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.0000236633.40160.ee"},{"key":"refg30\/ref30","doi-asserted-by":"publisher","DOI":"10.1159\/000205559"},{"key":"refg31\/ref31","doi-asserted-by":"publisher","DOI":"10.1160\/TH05-05-0313"},{"key":"refg33\/ref33","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2009.128678"},{"key":"refg32\/ref32","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.2008.26598"},{"key":"refg34\/ref34","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.325.7374.1202"},{"key":"refg35\/ref35","first-page":"443","author":"Wallerstein R.O.","year":"1987","journal-title":"West. J. Med."},{"key":"refg37\/ref37","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60854-X"},{"key":"refg36\/ref36","doi-asserted-by":"publisher","DOI":"10.1136\/oem.2010.063305"},{"key":"refg38\/ref38","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.49.9.755"},{"key":"refg39\/ref39","first-page":"775","volume":"54","author":"Yajnik C.S.","year":"2006","journal-title":"J. Assoc. Physicians India"},{"key":"refg40\/ref40","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.105.570846"}],"container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/full-xml\/10.1139\/cjpp-2014-0096","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/cjpp-2014-0096","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T12:59:18Z","timestamp":1578315558000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/cjpp-2014-0096"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9]]},"references-count":38,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2014,9]]}},"alternative-id":["10.1139\/cjpp-2014-0096"],"URL":"https:\/\/doi.org\/10.1139\/cjpp-2014-0096","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9]]}}}