{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:18:34Z","timestamp":1781104714551,"version":"3.54.1"},"reference-count":52,"publisher":"Canadian Science Publishing","issue":"7","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:p> Hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) is synthesized in perivascular adipose tissue (PVAT) and induces vasorelaxation. We examined whether the sulfur-containing AMP and GMP analogs AMPS and GMPS can serve as the H<jats:sub>2<\/jats:sub>S donors in PVAT. H<jats:sub>2<\/jats:sub>S production by isolated rat periaortic adipose tissue (PAT) was measured with a polarographic sensor. In addition, phenylephrine-induced contractility of aortic rings with (+) or without (\u2212) PAT was examined. Isolated PAT produced H<jats:sub>2<\/jats:sub>S from AMPS or GMPS in the presence of the P2X<jats:sub>7<\/jats:sub> receptor agonist BzATP. Phenylephrine-induced contractility of PAT(+) rings was lower than of PAT(\u2212) rings. AMPS or GMPS had no effect on the contractility of PAT(\u2212) rings, but used together with BzATP reduced the contractility of PAT(+) rings when endogenous H<jats:sub>2<\/jats:sub>S production was inhibited with propargylglycine. A high-fat diet reduced endogenous H<jats:sub>2<\/jats:sub>S production by PAT. Interestingly, AMPS and GMPS were converted to H<jats:sub>2<\/jats:sub>S by PAT of obese rats, and reduced contractility of PAT(+) aortic rings isolated from these animals even in the absence of BzATP. We conclude that (i) AMPS and GMPS can be hydrolyzed to H<jats:sub>2<\/jats:sub>S by PAT when P2X<jats:sub>7<\/jats:sub> receptors are activated, (ii) a high-fat diet impairs endogenous H<jats:sub>2<\/jats:sub>S production by PAT, (iii) AMPS and GMPS restore the anticontractile effects of PAT in obese animals without P2X<jats:sub>7<\/jats:sub> stimulation. <\/jats:p>","DOI":"10.1139\/cjpp-2014-0543","type":"journal-article","created":{"date-parts":[[2015,3,25]],"date-time":"2015-03-25T00:16:26Z","timestamp":1427242586000},"page":"585-595","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":21,"title":["Hydrogen-sulfide-mediated vasodilatory effect of nucleoside 5\u2032-monophosphorothioates in perivascular adipose tissue"],"prefix":"10.1139","volume":"93","author":[{"given":"Jerzy","family":"Be\u0142towski","sequence":"first","affiliation":[{"name":"Department of Pathophysiology, Medical University, ul. 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