{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T11:56:49Z","timestamp":1789732609606,"version":"4.0.1"},"reference-count":48,"publisher":"Canadian Science Publishing","issue":"1","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":["cdnsciencepub.com"],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:p> Nonsteroidal anti-inflammatory drugs (NSAIDs) constitute an important pharmacotherapeutic class that, over the past decade, have expanded in application to a panoply of medical conditions. They have been tested for neurodegenerative diseases such as Alzheimer\u2019s to reduce inflammation and also in the attempt to abrogate amyloid deposition. However, the use of NSAIDs as aggregation inhibitors has not been extensively studied in pancreatic amyloid deposition. Pancreatic amyloidosis involves the misfolding of islet amyloid polypeptide (IAPP) and contributes to the progression of type-2 diabetes in humans and felines. To ascertain their antiamyloidogenic activity, several NSAIDs were tested using fluorometric thioflavin-T assays, circular dichroism, photo-induced cross-linking assays, and cell culture. Celecoxib, diclofenac, indomethacin, meloxicam, niflumic acid, nimesulide, phenylbutazone, piroxicam, sulindac, and tenoxicam reduced fibrillization at a molar ratio of 1:10. The circular dichroism spectra of diclofenac, piroxicam, and sulindac showed characteristic spectral signatures found in predominantly \u03b1-helical structures. The oligomerization of human IAPP was abrogated with diclofenac and sulindac at a molar ratio of 1:5. The cytotoxic effects of pre-incubated human IAPP on cultured INS-1 cells were noticeably reduced in the presence of diclofenac, meloxicam, phenylbutazone, sulindac, and tenoxicam at a molar ratio of 1:10. Our results demonstrate that NSAIDs can provide chemical scaffolds to generate new and promising antiamyloidogenic agents that can be used alone or as a coadjuvant therapy. <\/jats:p>","DOI":"10.1139\/cjpp-2015-0117","type":"journal-article","created":{"date-parts":[[2015,6,17]],"date-time":"2015-06-17T21:37:38Z","timestamp":1434577058000},"page":"35-48","update-policy":"https:\/\/doi.org\/10.1139\/csp-crossmark-policy","source":"Crossref","is-referenced-by-count":17,"title":["Inhibition of islet amyloid polypeptide aggregation and associated cytotoxicity by nonsteroidal anti-inflammatory drugs"],"prefix":"10.1139","volume":"94","author":[{"given":"Jessica S.","family":"Fortin","sequence":"first","affiliation":[{"name":"D\u00e9partement de Pathologie et de Microbiologie, Facult\u00e9 de M\u00e9decine V\u00e9t\u00e9rinaire, Universit\u00e9 de Montr\u00e9al, 3200 Sicotte, Saint-Hyacinthe, QC J2S 2M2, Canada."},{"name":"D\u00e9partement de Pathologie et de Microbiologie, Facult\u00e9 de M\u00e9decine V\u00e9t\u00e9rinaire, Universit\u00e9 de Montr\u00e9al, 3200 Sicotte, Saint-Hyacinthe, QC J2S 2M2, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Odile","family":"Benoit-Biancamano","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Pathologie et de Microbiologie, Facult\u00e9 de M\u00e9decine V\u00e9t\u00e9rinaire, Universit\u00e9 de Montr\u00e9al, 3200 Sicotte, Saint-Hyacinthe, QC J2S 2M2, Canada."},{"name":"D\u00e9partement de Pathologie et de Microbiologie, Facult\u00e9 de M\u00e9decine V\u00e9t\u00e9rinaire, Universit\u00e9 de Montr\u00e9al, 3200 Sicotte, Saint-Hyacinthe, QC J2S 2M2, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2013.01.017"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.289.21.2819"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1021\/ar000214l"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1021\/jp511758w"},{"key":"refg5\/ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2006.04.008"},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1314481110"},{"key":"refg7\/ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2010.08.074"},{"key":"refg8\/ref8","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1305517110"},{"key":"refg9\/ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2012.02.042"},{"key":"refg10\/ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2013.06.029"},{"key":"refg11\/ref11","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1332.005"},{"key":"refg12\/ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2014.03.048"},{"issue":"2","key":"refg13\/ref13","doi-asserted-by":"crossref","first-page":"175","DOI":"10.18433\/J31G6C","volume":"13","author":"Fortin J.","year":"2010","journal-title":"J. Pharm. Pharm. Sci."},{"key":"refg14\/ref14","doi-asserted-by":"publisher","DOI":"10.3109\/13506129.2011.582902"},{"key":"refg15\/ref15","unstructured":"Goodman, L.S., Brunton, L.L., Chabner, B., and Knollmann, B.C. 2011. Goodman & Gilman\u2019s the pharmacological basis of therapeutics. McGraw-Hill Medical, New York; Toronto."},{"key":"refg16\/ref16","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.090532"},{"key":"refg17\/ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2011.12.062"},{"key":"refg18\/ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2011.08.006"},{"key":"refg19\/ref19","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(02)00266-6"},{"key":"refg20\/ref20","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4439(01)00078-3"},{"key":"refg21\/ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2007.01.022"},{"key":"refg22\/ref22","doi-asserted-by":"publisher","DOI":"10.2174\/138920310791330640"},{"key":"refg23\/ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2011.02.010"},{"key":"refg24\/ref24","unstructured":"Kahn, C.M., and Line, S. (Editors). 2010. The Merck veterinary manual, 10th ed. Merck & Co., Whitehouse Station, N.J."},{"key":"refg25\/ref25","doi-asserted-by":"publisher","DOI":"10.3109\/13506129.2014.937857"},{"key":"refg26\/ref26","doi-asserted-by":"publisher","DOI":"10.1155\/2008\/421287"},{"issue":"15","key":"refg27\/ref27","doi-asserted-by":"crossref","first-page":"5709","DOI":"10.1523\/JNEUROSCI.20-15-05709.2000","volume":"20","author":"Lim G.P.","year":"2000","journal-title":"J. Neurosci."},{"key":"refg28\/ref28","doi-asserted-by":"publisher","DOI":"10.1021\/bi701427q"},{"key":"refg29\/ref29","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2008.01.095"},{"key":"refg30\/ref30","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200900237"},{"issue":"3","key":"refg31\/ref31","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S0197-4580(98)00047-5","volume":"19","author":"Netland E.E.","year":"1998","journal-title":"Neurobiol. Aging"},{"key":"refg32\/ref32","doi-asserted-by":"publisher","DOI":"10.1177\/030098589303000401"},{"key":"refg33\/ref33","doi-asserted-by":"publisher","DOI":"10.1021\/jm010257n"},{"key":"refg34\/ref34","doi-asserted-by":"publisher","DOI":"10.1021\/bi0160462"},{"key":"refg35\/ref35","doi-asserted-by":"publisher","DOI":"10.3109\/13506129.2011.627956"},{"key":"refg36\/ref36","doi-asserted-by":"publisher","DOI":"10.1111\/j.1747-0285.2005.00318.x"},{"key":"refg37\/ref37","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2010.07.014"},{"key":"refg38\/ref38","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0703306104"},{"key":"refg39\/ref39","doi-asserted-by":"publisher","DOI":"10.1021\/jp5111604"},{"key":"refg40\/ref40","doi-asserted-by":"publisher","DOI":"10.3109\/13506129.2014.964858"},{"key":"refg41\/ref41","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(04)83013-1"},{"key":"refg42\/ref42","doi-asserted-by":"publisher","DOI":"10.1021\/bi3012548"},{"key":"refg43\/ref43","doi-asserted-by":"publisher","DOI":"10.1021\/cb500162w"},{"key":"refg44\/ref44","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00042.2009"},{"key":"refg45\/ref45","doi-asserted-by":"publisher","DOI":"10.1110\/ps.062486907"},{"issue":"20","key":"refg46\/ref46","doi-asserted-by":"crossref","first-page":"7504","DOI":"10.1523\/JNEUROSCI.23-20-07504.2003","volume":"23","author":"Yan Q.","year":"2003","journal-title":"J. Neurosci."},{"key":"refg47\/ref47","doi-asserted-by":"publisher","DOI":"10.1021\/bi800828u"},{"key":"refg48\/ref48","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2010.11.050"}],"container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdnsciencepub.com\/doi\/full-xml\/10.1139\/cjpp-2015-0117","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdnsciencepub.com\/doi\/pdf\/10.1139\/cjpp-2015-0117","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T14:07:23Z","timestamp":1751465243000},"score":1,"resource":{"primary":{"URL":"https:\/\/cdnsciencepub.com\/doi\/10.1139\/cjpp-2015-0117"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1]]},"references-count":48,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,1]]}},"alternative-id":["10.1139\/cjpp-2015-0117"],"URL":"https:\/\/doi.org\/10.1139\/cjpp-2015-0117","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,1]]},"assertion":[{"value":"2015-03-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-06-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-06-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}